Characterization of Hypericum perforatum polysaccharides with antioxidant and antimicrobial activities: Optimization based statistical modeling

Heydarian, Marzieh; Jooyandeh, Hossein; Nasehi, Behzad; Noshad, Mohammad

doi:10.1016/j.ijbiomac.2017.06.049

Cited by 42 publications

(20 citation statements)

References 23 publications

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“…Historic literature indicates a past appreciation for the oil; The Eclectic Dispensatory of the United States of America quoted it as being “a fine red balsamic ointment for wounds, ulcers, swellings, tumors, etc.” in 1852 (King, 1852 ), and Potter's Cyclopaedia of Botanical Drugs and Preparations described it as “a healing application to wounds, sores, ulcers, and swellings” to Londoners in 1907 (Wren, 1907 ). Recent research showed that polysaccharides from H. perforatum have antimicrobial activity against Escherichia coli, Shigella dysenteriae, Salmonella typhi, Bacillus cereus , and Staphylococcus aureus when tested by a disk diffusion assay (Heydarian et al, 2017 ). Another study demonstrated H. perforatum extracts and partitions had antibacterial activity against several oral bacteria including Streptococcus mutans, S. sobrinus, Lactobacillus plantarum , and Enterococcus faecalis (Suntar et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

The Chemical and Antibacterial Evaluation of St. John's Wort Oil Macerates Used in Kosovar Traditional Medicine

et al. 2017

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Hypericum perforatum L. (Hypericaceae), or St. John's Wort, is a well-known medicinal herb often associated with the treatment of anxiety and depression. Additionally, an oil macerate (Oleum Hyperici) of its flowering aerial parts is widely used in traditional medicine across the Balkans as a topical wound and ulcer salve. Other studies have shown that Oleum Hyperici reduces both wound size and healing time. Of its active constituents, the naphthodianthrone hypericin and phloroglucinol hyperforin are effective antibacterial compounds against various Gram-positive bacteria. However, hyperforin is unstable with light and heat, and thus should not be present in the light-aged oil macerate. Additionally, hypericin can cause phototoxic skin reactions if ingested or absorbed into the skin. Therefore, the established chemistry presents a paradox for this H. perforatum oil macerate: the hyperforin responsible for the antibacterial bioactivity should degrade in the sunlight as the traditional oil is prepared; alternately, if hypericin is present in established bioactive levels, then the oil macerate should cause photosensitivity, yet none is reported. In this research, various extracts of H. perforatum were compared to traditional oil macerates with regards to chemical composition and antibacterial activity (inhibition of growth, biofilm formation, and quorum sensing) vs. several strains of Staphylococcus aureus in order to better understand this traditional medicine. It was found that four Kosovar-crafted oil macerates were effective at inhibiting biofilm formation (MBIC50 active range of 0.004–0.016% v/v), exhibited moderate inhibition of quorum sensing (QSIC50 active range of 0.064–0.512% v/v), and contained detectable amounts of hyperforin, but not hypericin. Overall, levels of hypericin were much higher in the organic extracts, and these also exhibited more potent growth inhibitory activity. In conclusion, these data confirm that oil macerates employed in traditional treatments of skin infection lack the compound credited with phototoxic reactions in H. perforatum use and exhibit anti-biofilm and modest quorum quenching effects, rather than growth inhibitory properties against S. aureus.

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Section: Introductionmentioning

confidence: 99%

The Chemical and Antibacterial Evaluation of St. John's Wort Oil Macerates Used in Kosovar Traditional Medicine

et al. 2017

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“…The significance of the lack of fit test indicated that the points were not properly distributed around the model; as a result, the model could not be applied to predict the values of the independent variables. Therefore, the insignificance of the lack of fit test implied that the model was able to fit the data properly [ 13 ]. In our models, all the values of “ p -value prob > F ” of the lack of fit were greater than 10% and they were insignificant.…”

Section: Resultsmentioning

confidence: 99%

“…The Box–Behnken design response surface methodology (BBD-RSM) is a collection of mathematical and statistical techniques useful for establishing models which can be used to evaluate multiple parameters and their interactions with quantitative data, and effectively optimizing complex extraction procedures in a statistical way [ 13 , 14 , 15 ]. It reduces not only the number of experimental trials, the development time, and overall cost determines, but also the interactions among the independent variables [ 16 , 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Optimisation of Ethanol-Reflux Extraction of Saponins from Steamed Panax notoginseng by Response Surface Methodology and Evaluation of Hematopoiesis Effect

Cui

Zhang

et al. 2018

Molecules

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The present study aims to optimize the ethanol-reflux extraction conditions for extracting saponins from steamed Panax notoginseng (SPN). Four variables including the extraction time (0.5–2.5 h), ethanol concentration (50–90%), water to solid ratio (W/S, 8–16), and times of extraction (1–5) were investigated by using the Box-Behnken design response surface methodology (BBD-RSM). For each response, a second-order polynomial model with high R2 values (>0.9690) was developed using multiple linear regression analysis and the optimum conditions to maximize the yield (31.96%), content (70.49 mg/g), and antioxidant activity (EC50 value of 0.0421 mg/mL) for saponins extracted from SPN were obtained with a extraction time of 1.51 h, ethanol concentration of 60%, extraction done 3 times, and a W/S of 10. The experimental values were in good consistency with the predicted ones. In addition, the extracted SPN saponins could significantly increase the levels of blood routine parameters compared with the model group (p < 0.01) and there was no significant difference in the hematopoiesis effect between the SPN group and the SPN saponins group, of which the dose was 15 times lower than the former one. It is suggested that the SPN saponins extracted by the optimized method had similar functions of “blood tonifying” at a much lower dose.

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“…The antioxidant capacities of serum and thymus, including T-SOD, T-AOC, CAT, GSH-Px, and MDA, were measured using an antioxidant index measurement kit (Nanjing Jiancheng Bioengineering Institute, China) (Heydarian, Jooyandeh, Nasehi, & Noshad, 2017).…”

Section: Antioxidant Activitymentioning

confidence: 99%

Optimization of the extraction process of polysaccharides from Dendrobium officinale and evaluation of the in vivo immunmodulatory activity

Wang

Guo

et al. 2018

J Food Process Preserv

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Dendrobium officinale (D. officinale) was regarded as the nutritious food in Asia for the remarkable bioactivities on immunmodulatory. Dendrobium officinale polysaccharide (DOP) served as the main active compound in the herb. Extraction of DOP was investigated and immunmodulatory activities were evaluated. The optimal extraction process (liquid‐to‐material ratio of 24.6:1, extraction time of 2.1 hr, and temperature of 67.4 °C) was obtained by single‐factor analysis and response surface optimization. Under this condition, DOP reached the extraction yield of 92.42%. Furthermore, DOP can promote the proliferation of splenic lymphocytes of immunodeficient mice and enhance the phagocytic capability of peritoneal macrophages. The contents of IL‐6 and TNF‐α in serum and splenocyte supernatants were significantly upregulated. Similarly, the contents of immune globulins (IgM and IgG) and hemolysis in serum was increased, as well as the antioxidant capabilities of thymus and serum. Therefore, this work gave an effective extraction method for DOP, and elucidated the immunmodulatory mechanisms of DOP at the level of the animal. Practical applications Dendrobium officinale polysaccharide (DOP) is active compounds in Dendrobium officinale (D. officinale), which was widely used as nutritious food in Asia for its remarkable bioactivities such as the curative effect on chronic atrophic gastritis and hypertension and diabetes, tumor‐suppression, and anti‐aging effects. In the current study, it optimized the extraction process to acquire high‐quality and stable DOP. Furthermore, it explored the immunoregulatory mechanism of DOP using immunosuppressive mouse model. This work contributed to the further development and utilization of DOP as a novel nutritious food additive component.

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Characterization of Hypericum perforatum polysaccharides with antioxidant and antimicrobial activities: Optimization based statistical modeling

Cited by 42 publications

References 23 publications

The Chemical and Antibacterial Evaluation of St. John's Wort Oil Macerates Used in Kosovar Traditional Medicine

The Chemical and Antibacterial Evaluation of St. John's Wort Oil Macerates Used in Kosovar Traditional Medicine

Optimisation of Ethanol-Reflux Extraction of Saponins from Steamed Panax notoginseng by Response Surface Methodology and Evaluation of Hematopoiesis Effect

Optimization of the extraction process of polysaccharides from Dendrobium officinale and evaluation of the in vivo immunmodulatory activity

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