Characterization of hydrogen production by an ammonium insensitive Pseudo-revertant of a CBB deficient Rhodobacter capsulatus strain

Potential of Phototrophic Purple Nonsulfur Bacteria to Fix Nitrogen in Rice Fields

“…When nifA gene was overexpressed in a mutant of R. sphaeroides, hydrogen production was detected in the presence of 14 mM ammonium [143]. A point mutation in nifA enabled hydrogen production in the presence of ammonium in a R. capsulatus mutant [144] and a R. sphaeroides strain harboring a vector expressing the nifA L62Q allele [145]. R. palustris draT2 deletion or glnK2 deletion in addition to the 48-nucleotide deletion in nifA improved ammonium tolerance and produced hydrogen under higher ammonium concentrations, compared to those observed with only the 48-nucleotide deletion in nifA [146].…”

Draft Genome Sequences of Two Heat-Resistant Mutant Strains (A52 and B41) of the Photosynthetic Hydrogen-Producing Bacterium Rhodobacter capsulatus

“…Rhodobacter capsulatus is a purple alphaproteobacterium known for its metabolic plasticity, enabling it to thrive in disparate ecological niches ( 1 , 2 ). Furthermore, this feature of R. capsulatus makes it an ideal model to study respiratory, photosynthetic, and chemolithotrophic growth modes ( 1 – 3 ). In the last two decades, studies on R. capsulatus particularly focused on the ability of this organism to produce hydrogen ( 3 – 5 ).…”

“…Furthermore, this feature of R. capsulatus makes it an ideal model to study respiratory, photosynthetic, and chemolithotrophic growth modes ( 1 – 3 ). In the last two decades, studies on R. capsulatus particularly focused on the ability of this organism to produce hydrogen ( 3 – 5 ). In order to achieve sustainable hydrogen production in outdoor photobioreactors, two heat-resistant mutant strains (A52 and B41) of R. capsulatus DSM 1710 have been developed by our group through a directed evolution approach, using chemical mutagenesis ( 5 ).…”