Characterization of hydrangea chlorotic mottle virus, a new member of the genus Carlavirus

Tang, Joe; Harper, Scott J.; Wei, Ting; Clover, G. R. G.

doi:10.1007/s00705-009-0541-3

Cited by 8 publications

(4 citation statements)

References 22 publications

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“…The p12-induced hyperplasia might account for the leaf malformation symptoms observed in infections caused by some other carlaviruses (Hakkaart and Maat, 1974;Dijkstra et al, 1985;Larsen et al, 2009;Tang et al, 2010). Although leaf malformation is a quite common symptom induced by many carlaviruses, to the best of our knowledge, none of the hostcarlavirus pathosystems other than CVB have been investigated for hyperplasia induction and this awaits characterization.…”

Section: Results and Discussion P12 Expression Causes Severe Leaf Malmentioning

confidence: 99%

An RNA Virus-Encoded Zinc-Finger Protein Acts as a Plant Transcription Factor and Induces a Regulator of Cell Size and Proliferation in Two Tobacco Species

et al. 2013

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Plant viruses cause a variety of diseases in susceptible hosts. The disease symptoms often include leaf malformations and other developmental abnormalities, suggesting that viruses can affect plant development. However, little is known about the mechanisms underlying virus interference with plant morphogenesis. Here, we show that a C-4 type zinc-finger (ZF) protein, p12, encoded by a carlavirus (chrysanthemum virus B) can induce cell proliferation, which results in hyperplasia and severe leaf malformation. We demonstrate that the p12 protein activates expression of a regulator of cell size and proliferation, designated upp-L (upregulated by p12), which encodes a transcription factor of the basic/helix-loop-helix family sufficient to cause hyperplasia. The induction of upp-L requires translocation of the p12 protein into the nucleus and ZF-dependent specific interaction with the conserved regulatory region in the upp-L promoter. Our results establish the role of the p12 protein in modulation of host cell morphogenesis. It is likely that other members of the conserved C-4 type ZF family of viral proteins instigate reprogramming of plant development by mimicking eukaryotic transcriptional activators.

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Section: Results and Discussion P12 Expression Causes Severe Leaf Malmentioning

confidence: 99%

An RNA Virus-Encoded Zinc-Finger Protein Acts as a Plant Transcription Factor and Induces a Regulator of Cell Size and Proliferation in Two Tobacco Species

et al. 2013

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“…During the last five years, a number of viral species belonging to the family Betaflexiviridae have been characterized by either classical approaches (e.g. [1–9]) or using high-throughput next generation sequencing (NGS) technologies [10–12]. In the later case, different strategies have been used, targeting different nucleic acid templates (single-stranded or double-stranded RNAs, small interfering RNAs, messenger RNAs, partially or completely purified viral particles) or the sequencing technology (Illumina or 454 pyrosequencing [13–17]).…”

Section: Introductionmentioning

confidence: 99%

Characterization of New Isolates of Apricot vein clearing-associated virus and of a New Prunus-Infecting Virus: Evidence for Recombination as a Driving Force in Betaflexiviridae Evolution

et al. 2015

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Double stranded RNAs from Prunus samples gathered from various surveys were analyzed by a deep-sequencing approach. Contig annotations revealed the presence of a potential new viral species in an Azerbaijani almond tree (Prunus amygdalus) and its genome sequence was completed. Its genomic organization is similar to that of the recently described Apricot vein clearing associated virus (AVCaV) for which two new isolates were also characterized, in a similar fashion, from two Japanese plums (Prunus salicina) from a French germplasm collection. The amino acid identity values between the four proteins encoded by the genome of the new virus have identity levels with those of AVCaV which fall clearly outside the species demarcation criteria. The new virus should therefore be considered as a new species for which the name of Caucasus prunus virus (CPrV) has been proposed. Phylogenetic relationships and nucleotide comparisons suggested that together with AVCaV, CPrV could define a new genus (proposed name: Prunevirus) in the family Betaflexiviridae. A molecular test targeting both members of the new genus was developed, allowing the detection of additional AVCaV isolates, and therefore extending the known geographical distribution and the host range of AVCaV. Moreover, the phylogenetic trees reconstructed with the amino acid sequences of replicase, movement and coat proteins of representative Betaflexiviridae members suggest that Citrus leaf blotch virus (CLBV, type member of the genus Citrivirus) may have evolved from a recombination event involving a Prunevirus, further highlighting the importance of recombination as a driving force in Betaflexiviridae evolution. The sequences reported in the present manuscript have been deposited in the GenBank database under accession numbers KM507061-KM504070.

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“…Necrosis of the epidermal cells is observed in response to the mycopathogen, possibly due to hypersensitive response. ocasionadas por nemátodos (French, John, & Williams, 1971;Basterrechea, 2005), virus (Tang, Harper, Wei, & Clover, 2010;Dória, Nozaki, Pavan, Yuki, & Sakate, 2011). Hongos (Hagan, Olive, Stephenson, & Rivas-Davila, 2004;Freire & Mosca, 2009;Windham et al, 2011;Mmbaga, Kim, Mackasmiel, & Li, 2012).…”

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confidence: 99%

Alteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae)

Barón

Grisales-Echeverri

Cuarán

et al. 2020

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Introducción: Los estudios sobre las alteraciones morfo-anatómicas e histoquímicas ocasionadas por la Oidiosis o ceniza en hojas de Hydrangea macrophylla son escasos en la literatura científica. Objetivos: describir y analizar aspectos anatómicos e histopatológicos de este patosistema. Métodos: se tomaron más de 90 hojas de H. macrophylla sanas e infectadas por Oidiosis que se recolectaron en el vivero El Jardín del Edén, Rionegro, Antioquia-Colombia. Para la identificación del micopatógeno, las secciones obtenidas se tiñeron con azul de Lactofenol y se consultaron claves taxonómicas especializadas. Fragmentos transversales de 1 cm de grosor que se fijaron en una mezcla de formol, alcohol y ácido acético. Posteriormente, se deshidrataron en una serie de etanol, se aclararon en Xilol y finalmente, se incluyeron en Paraplast plus® para obtener secciones de 5 μm. Se utilizó la reacción del ácido peryódico de Schiff (PAS) para detectar polipolisacáridos, rojo de Rutenio para pectinas, Ponseau S y Lacmoid para calosa, Cloruro férrico para polifenoles, negro de Sudan para lípidos y Uvitex 2B-Hematoxilina para quitina. Las secciones se observaron mediante un microscopio fotónico Nikon 80i eclipse® y con epifluorescencia empleando filtro UV-2A. Para la observación con microscopía electrónica de barrido, hojas sanas e infectadas fueron fijadas y deshidratadas en metanol al 100 %, desecadas al punto crítico y recubiertas con oro. Resultados: las hojas de H. macropylla son dorsiventrales, homobáricas, con epidermis adaxial y abaxial de un solo estrato celular. El parénquima de empalizada está constituido por un estrato de células cortas. En tanto que el parénquima esponjoso forma entre 6-7 estratos celulares. Todos los haces vasculares en la lámina foliar son colaterales cerrados. Se aprecian abundantes idioblastos en el mesófilo con rafidios; el almidón es el principal carbohidrato. Las hojas son hipostomáticas de patrones paracíticos, estos son superficiales y con amplias cavidades subestomáticas. Los datos morfológicos apreciados en el micopatógeno, están relacionados con el género Erysiphe. Las células epidérmicas afectadas por el patógeno se aprecian con pared engrosada, citoplasma granular y papilas o aposiciones de la pared celular presentes en las paredes periclinales externas. Con el deterioro de la epidermis, los tejidos subyacentes se afectan y se aprecian necróticos. Los análisis histoquímicos demuestran que las plantas infectadas engrosan y refuerzan sus paredes celulares epidérmicas con materiales de pared primaria, principalmente, cutina, pectinas y calosa. La presencia de aglomerados de color oscuro teñidos con el Sudan negro, en el citoplasma de las células epidérmicas, podrían relacionarse con mecanismos de defensa de la planta y los observados en las células del mesófilo con la desorganización de los sistemas de membrana. Los polifenoles se acumulan en el citoplasma de las células epidérmicas infectadas. El material fúngico presente en los tejidos epidérmicos fue diferenciado claramente al ser teñido con el fluorocromo para quitina. Conclusiones: El agente causal de la Oidiosis o ceniza en H. macrophylla está relacionado con especies del género Erysiphe. Se aprecia necrosis de las células epidérmicas en respuesta al patógeno, que podría estar relacionado con respuestas de hipersensibilidad.

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Characterization of hydrangea chlorotic mottle virus, a new member of the genus Carlavirus

Cited by 8 publications

References 22 publications

An RNA Virus-Encoded Zinc-Finger Protein Acts as a Plant Transcription Factor and Induces a Regulator of Cell Size and Proliferation in Two Tobacco Species

An RNA Virus-Encoded Zinc-Finger Protein Acts as a Plant Transcription Factor and Induces a Regulator of Cell Size and Proliferation in Two Tobacco Species

Characterization of New Isolates of Apricot vein clearing-associated virus and of a New Prunus-Infecting Virus: Evidence for Recombination as a Driving Force in Betaflexiviridae Evolution

Alteraciones anatómicas e histoquímicas ocasionadas por la oidiosis en hojas de Hydrangea macrophylla (Hydrangeaceae)

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