Characterization of human sperm antigens reacting with antisperm antibodies from autologous sera and seminal plasma in a fertile population

Paradisi, Roberto; Pession, Annalisa; Bellavia, Emanuela; Focacci, M.; Flamigni, Carlo

doi:10.1016/0165-0378(94)00911-p

Cited by 16 publications

(23 citation statements)

References 26 publications

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“…A spermatozoon would have no obvious use for such a transfer mechanism. Anti-sperm IgA can be secreted in both the female and male reproductive tracts leading to infertility (Bohring & Krause 2005, Francavilla et al 2007; however, anti-sperm antibodies have been detected in seminal plasma of up to 62% of fertile men (Paradisi et al 1995) and in cervico-vaginal secretions of a small percentage of fertile women (Mahdi et al 2011). If confirmed to be present and indeed a functional receptor in sperm, it is possible that PigR mediates the interaction between spermatozoa and low levels of secreted IgA encountered en route to fertilisation; the significance of such an interaction is not clear at the present time.…”

Section: Study Of Stallion Sperm Proteome By Lc-ms/msmentioning

confidence: 99%

Investigation of the stallion sperm proteome by mass spectrometry

Swegen¹,

Curry²,

Gibb³

et al. 2015

Reproduction

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Stallion spermatozoa continue to present scientific and clinical challenges with regard to the biological mechanisms responsible for their survival and function. In particular, deeper understanding of sperm energy metabolism, defence against oxidative damage and cell-cell interactions should improve fertility assessment and the application of advanced reproductive technologies in the equine species. In this study, we used highly sensitive LC-MS/MS technology and sequence database analysis to identify and characterise the proteome of Percoll-isolated ejaculated equine spermatozoa, with the aim of furthering our understanding of this cell's complex biological machinery. We were able to identify 9883 peptides comprising 1030 proteins, which were subsequently attributed to 975 gene products. Gene ontology analysis for molecular and cellular processes revealed new information about the metabolism, antioxidant defences and receptors of stallion spermatozoa. Mitochondrial proteins and those involved in catabolic processes constituted dominant categories. Several enzymes specific to b-oxidation of fatty acids were identified, and further experiments were carried out to ascertain their functional significance. Inhibition of carnitine palmitoyl transferase 1, a rate-limiting enzyme of b-oxidation, reduced motility parameters, indicating that b-oxidation contributes to maintenance of motility in stallion spermatozoa.

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Section: Study Of Stallion Sperm Proteome By Lc-ms/msmentioning

confidence: 99%

Investigation of the stallion sperm proteome by mass spectrometry

Swegen¹,

Curry²,

Gibb³

et al. 2015

Reproduction

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“…The sperm membrane is composed of a complex mosaic of oligosaccharides and lipids in addition to proteins [21]. For the purpose of the present study, considering that sperm antibodies are widely accepted as present in both the fertile and the infertile population [1,3,21], we employed Western blotting-a very effective and sensitive assay able to distinguish in an essentially qualitative way the specific and discrete sperm antigens of fertile or infertile individuals recognized by selective circulating and/or local antibodies. Only marginal consideration was given to the quantitatively enhanced immunogenicity of sperm from infertile subjects, which has already been documented [6,7,10].…”

Section: Discussionmentioning

confidence: 99%

“…The second group was composed of 10 subjects with no significant abnormalities in the semen analysis (Table 1). For comparison, a group of 55 individuals with proven fertility and normal semen quality served as a control fertile population [21].…”

Section: Subjects and Sample Selectionmentioning

confidence: 99%

“…Sperm cells were treated as previously reported [21]. Briefly, they were washed twice with Dulbecco's PBS and resuspended in extraction buffer containing 10 mM Tris (pH 7.2), 0.15 M NaCl, 0.02% NaN 3 , 0.5% Nonidet P 40 (Bio-Rad, Richmond, VA), and 1 mM PMSF for 1-2 h.…”

Section: Extraction and Preparation Of Sperm Antigensmentioning

confidence: 99%

“…In our recent paper [21], we characterized some discrete and specific proteins present in the normal human sperm cell in an attempt to construct a preliminary map of the antigens of the cell. Serum and seminal plasma of each individual were employed and subjected to reaction with their own respective antigenic sources, in a large population with proven fertility.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Human Sperm Antigens Reacting with Sperm Antibodies from Autologous Serum and Seminal Plasma in an Infertile Population1

Paradisi¹,

Bellavia²,

Pession

et al. 1996

Biology of Reproduction

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Immunoblotting techniques were used to characterize the reactivity of human sperm antigens with sperm antibodies from an infertile population. Sperm antigens of each individual were tested with autologous sperm antibodies present in serum and seminal plasma in order to construct a preliminary map of the antigens of the infertile spermatozoon and to compare the qualitative differences in the antigenic profile between fertile and infertile subjects. A total of 61 infertile males, comprising 51 subjects having poor semen quality and 10 subjects with no abnormalities in semen analysis, entered the study; 55 subjects with proven fertility served as controls. Infertile subjects often showed specific immunoreactivity to 50-, 55-, 57-, 62-, and 72-kDa proteins in serum and to 57- and 62-kDa proteins in seminal plasma. As to comparison of immunoreactivities between fertile and infertile individuals, the sperm antigens may be divided into three groups. Group 1 antigens (50-, 69-, and 72-kDa proteins) were recognized by sperm antibodies present in both populations; group 2 antigens (57- and 62-kDa proteins), by sperm antibodies typical of the infertile population; group 3 antigens (45-, 55-, and 85-kDa proteins), by sperm antibodies typical of the fertile population. This classification shows that the infertile spermatozoon differs substantially at the immunogenic level from the fertile spermatozoon. The group 2 antigens seem to be involved in a relevant step in the reproductive process and hence have been termed "fertility-related antigens."

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