Characterization of human sperm antigens and antisperm antibodies in infertile patients

Snow, K. M.; Ball, G.D.

doi:10.1016/0020-7292(93)90475-c

Cited by 6 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All semen samples positive for U. urealyticum and anti-sperm antibodies were excluded. The spermatozoa were prepared, and membrane proteins were extracted according to the method of Snow (31). Samples were then subjected to electrophoretic analysis and preparation for anti-SMP antiserum.…”

Section: Methodsmentioning

confidence: 99%

Screening of an Antigen Target for Immunocontraceptives from Cross-Reactive Antigens between Human Sperm andUreaplasma urealyticum

et al. 2007

View full text Add to dashboard Cite

Epidemiologic studies indicated that some infertile men who were infected with Ureaplasma urealyticum displayed positive antisperm antibodies in their serum and/or semen. The purpose of this study was to investigate the possible mechanism of antisperm antibodies production after infection with U. urealyticum and to analyze the relationship between U. urealyticum and infertility. The existence of cross-reactive antigens (61, 50, and 25 kDa) between U. urealyticum and human sperm membrane proteins was confirmed. Among the cross-reactive antigens, the urease complex component UreG of U. urealyticum was determined. By searching the Swiss-Prot protein database, a pentapeptide identity (IERLT) between UreG and human nuclear autoantigenic sperm protein (NASP) was found. Furthermore, using Western blot analysis and enzyme-linked immunosorbent assay, the cross-reaction between the NASP and UreG was verified. Both anti-rUreG antibody and the antiserum against the synthetic peptide NASP393-408 containing the pentapeptide inhibited mouse sperm egg binding and fusion. After immunization by rUreG or the synthetic peptide, 81.2 and 75% female mice became sterile, respectively. The effect on fertility in mice immunized with the synthetic peptide was reversible. These findings proved for the first time that it was feasible to screen antigens for immunocontraceptives from cross-reactive antigens between sperm and microorganisms which induce infertility.Ureaplasma urealyticum lacks cell wall, belongs to the taxonomic class Mollicutes, and is one of the smallest self-replicating prokaryotes (33). Among Mollicutes, U. urealyticum is unique in its ability to hydrolyze urea and requires urea to support growth (16). In 1954, Shepard first isolated U. urealyticum from the urethras of patients with nongonococcal urethritis (29). Since then, there has been intense interest in determining what role U. urealyticum might play in human diseases, particularly in the pathogenesis of genital tract infectious diseases.Because U. urealyticum is an inhabitant of the human lower genital tract, infections are considered to be sexually transmitted and occur more frequently during fertile ages. Although the contributory role of U. urealyticum in infertility has yet to be conclusively established, considerable data have been compiled to support the theory that U. urealyticum can cause infertility (10,11,40,41). Early epidemiologic studies have indicated that U. urealyticum was linked to human reproductive failure on the basis of higher frequencies of isolation from infertile versus fertile couples (11). Friberg and Gnarpe (10) found that U. urealyticum existed in the semen of 76% of the husbands from 50 couples with unexplained infertility. Xu et al. (40) also observed significantly higher frequency of U. urealyticum infection among infertile males (549 of 1,416) compared to fertile controls (34 of 375). Furthermore, they demonstrated that U. urealyticum not only could adhere to the membrane of spermatozoa but also could interfere with sperm motility an...

show abstract

Section: Methodsmentioning

confidence: 99%

Screening of an Antigen Target for Immunocontraceptives from Cross-Reactive Antigens between Human Sperm andUreaplasma urealyticum

et al. 2007

View full text Add to dashboard Cite

show abstract

“…By using immunoblotting we have found a spectrum of sperm antigens which were bound to antisperm antibodies contained in serum samples from the studied populations. There are numerous studies reporting the reactions between ASA and sperm antigens (Primakoff et al ., 1990; Snow & Ball, 1992; de Beer et al ., 1993; D’Cruz et al ., 1993; Diekman & Goldberg, 1994; Auer et al ., 1995; Paradisi et al ., 1996a; Paradisi et al ., 1996b; Auer et al ., 1997). The previously obtained results, however, have not been consistent.…”

Section: Discussionmentioning

confidence: 99%

Sperm antigens recognized by antisperm antibodies present in sera of infertile adults and prepubertal boys with testicular failure

2000

View full text Add to dashboard Cite

Immunoblotting of a repertoire of sperm antigens reacting with antisperm antibodies present in sera of infertile adults and prepubertal boys with testicular failure was performed. In the subgroups selected for this study, 55% of examined infertile women, 65% of infertile men and 64% of prepubertal boys with gonadal failure gave positive results by Western blotting with extracted sperm antigens. Sperm antigens with molecular weights of 57, 58, 62, 63 and 66 kDa were the most immunodominant entities recognized by antisperm antibodies from prepubertal boys. No positive reactions were detected by Western blotting in a control population of fertile adults, whereas in a group of prepubertal healthy boys only one sample revealed reactivity against sperm antigens of 58 and 70 kDa.

show abstract

“…The procedure applied for isolation of cell surface antigens has been previously described 9 . Briefly, the cells were incubated for 1 hr in an ice bath, in a buffer containing 1% v/v of Triton X‐100, 1% w/v of sodium deoxycholate, 0.05 m NaCl, 0.02 m Tris (pH 7.8) (TDOC buffer) 3 and protease inhibitors. After centrifugation, the supernatant, containing biotin‐labelled surface antigenic mixture, was recovered and either immediately used or stored at −70°C.…”

Section: Preparation Of Antigenic Extracts From the Biotin‐labelled Cmentioning

confidence: 99%

“…Many Western immunoblotting and few immunoprecipitation studies have been focused on identification of auto‐ and isoimmune reactive sperm antigens 2–10 . However, the molecular weights (Mw) of anti‐sperm antibody‐reactive sperm antigens, resulting from these studies were not always consistent.…”

Section: Introductionmentioning

confidence: 99%

Immunoprecipitation of Sperm and Somatic Antigens with Antibodies from Sera of Sperm‐Sensitized and Anti‐sperm Antibody‐ Free Individuals

Domagala

Kurpisz

2004

American J Rep Immunol

View full text Add to dashboard Cite

show abstract

Characterization of human sperm antigens and antisperm antibodies in infertile patients

Cited by 6 publications

References 0 publications

Screening of an Antigen Target for Immunocontraceptives from Cross-Reactive Antigens between Human Sperm andUreaplasma urealyticum

Screening of an Antigen Target for Immunocontraceptives from Cross-Reactive Antigens between Human Sperm andUreaplasma urealyticum

Sperm antigens recognized by antisperm antibodies present in sera of infertile adults and prepubertal boys with testicular failure

Immunoprecipitation of Sperm and Somatic Antigens with Antibodies from Sera of Sperm‐Sensitized and Anti‐sperm Antibody‐ Free Individuals

Contact Info

Product

Resources

About