Characterization of Human Renal Fibroblasts in Health and Disease: I. Immunophenotyping of Cultured Tubular Epithelial Cells and Fibroblasts Derived From Kidneys With Histologically Proven Interstitial Fibrosis

Ga, Müller; Hp, Rodemann

doi:10.1016/s0272-6386(12)80351-9

Cited by 83 publications

(47 citation statements)

References 10 publications

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“…Conversely, in tissue fibrosis, fibroblasts keep their activated state, often until organ death. So fibroblasts isolated from fibrotic tissue maintain their activated phenotype when cultured in vitro (Orimo et al 2005;Muller and Rodemann 1991). Considering all of that information, it seems to be expected that fibroblasts from scar presented an advantage regarding proliferative rate and survival advantages compared with fibroblasts obtained from other sites used in this work, but more studies are necessary to verify this findings, including other body sites and expression profile of fibroblast factors.…”

Section: Discussionmentioning

confidence: 71%

“…So far, fibroblasts are considered the main mediators of tissue fibrosis and scar formation (Kalluri and Zeisberg 2006). In addition, in wound healing, fibroblasts secret higher levels of ECM constituents and proliferate more than in healthy organs (Muller and Rodemann 1991;Castor et al 1979). This amplified activity suggests cell activation processes (Castor et al 1979).…”

Section: Discussionmentioning

confidence: 99%

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Fibroblast sources: Where can we get them?

et al. 2014

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Fibroblasts are cells widely used in cell culture, both for transient primary cell culture or permanent as transformed cell lines. Lately, fibroblasts become cell sources for use in disease modeling after cell reprogramming because it is easily accessible in the body. Fibroblasts in patients will maintain all genetic background during reprogramming into induced pluripotent stem cells. In spite of their large use, fibroblasts are obtained after an invasive procedure, a superficial punch skin biopsy, collected under patient's local anesthesia. Taking into consideration the minimum patient's discomfort during and after the biopsy procedure, as well as the aesthetics aspect, it is essential to reflect on the best site of the body for the biopsy procedure combined with the success of getting robust fibroblast cultures in the lab. For this purpose, we compared the efficiency of four biopsy sites of the body (skin from eyelid, back of the ear, abdominal cesarean scar and groin). Cell proliferation assays and viability after cryopreservation were measured. Our results revealed that scar tissue provided fibroblasts with higher proliferative rates. Also, fibroblasts from scar tissues presented a higher viability after the thawing process.

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Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Fibroblast sources: Where can we get them?

et al. 2014

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“…Following recurrent tissue injury, myofibroblasts undergo reactivation (42,43). Although data on the reversibility of fibrotic processes in the kidney are scarce, few studies indicate that resolution of renal fibrosis does exist (44)(45)(46).…”

Section: Discussionmentioning

confidence: 99%

Selective delivery of IFN‐γ to renal interstitial myofibroblasts: a novel strategy for the treatment of renal fibrosis

et al. 2014

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Renal fibrosis leads to end-stage renal disease demanding renal replacement therapy because no adequate treatment exists. IFN-g is an antifibrotic cytokine that may attenuate renal fibrosis. Systemically administered IFN-g causes side effects that may be prevented by specific drug targeting. Interstitial myofibroblasts are the effector cells in renal fibrogenesis. Here, we tested the hypothesis that cell-specific delivery of IFN-g to plateletderived growth factor receptor b (PDGFRb)-expressing myofibroblasts attenuates fibrosis in an obstructive nephropathy [unilateral ureteral obstruction (UUO)] mouse model. PEGylated IFN-g conjugated to PDGFRbrecognizing peptide [(PPB)-polyethylene glycol (PEG)-IFN-g] was tested in vitro and in vivo for antifibrotic properties and compared with free IFN-g. PDGFRb expression was >3-fold increased (P < 0.05) in mouse fibrotic UUO kidneys and colocalized with a-smooth muscle actin-positive (SMA + ) myofibroblasts.

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“…Macrophages play an important role in renal tissue damage through production of proteolytic enzymes, cytokines, and growth factors such as interleukin-1 (IL-1), tumor necrosis factor-e((TNF-(x), transforming growth factor-I} (TGF-I~), and plateletderived growth factor (PDGF) (1)(2)(3). These factors induce proliferation of fibroblasts leading to extracellular matrix fibrosis.…”

Section: Introductionmentioning

confidence: 99%

Identification and immunohistochemical localization of macrophage migration inhibitory factor in human kidney

et al. 1996

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SUMMARY:Macrophage migration inhibitory factor (MIF) was the first lymphokine identified in activated T-lymphocytes. MIF can induce proinflammatory cytokines, such as interleukin-1 and tumor necrosis factor-(~. In this study, we identified MIF expression in a tissue specimen of a normal portion of a nephrectomized human kidney by reverse transcription-polymerase chain reaction (RT-PCR) and Westem blot analysis. Furthermore, immunohistochemical study using an anti-human MIF polyclonal antibody demonstrated that MIF was mostly present in the renal tubule epithelial cells and, to a lesser extent, in Bowman's capsular epithelial cells. We also carried out immunohistochemistry on cultured human renal proximal tubule epithelial cells, which showed that MIF was present in the cytoplasm of the epithelial cells. These results suggest the possibility that MIF takes part in the mechanism of inflammation and immunological events in the human kidney:

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Characterization of Human Renal Fibroblasts in Health and Disease: I. Immunophenotyping of Cultured Tubular Epithelial Cells and Fibroblasts Derived From Kidneys With Histologically Proven Interstitial Fibrosis

Cited by 83 publications

References 10 publications

Fibroblast sources: Where can we get them?

Fibroblast sources: Where can we get them?

Selective delivery of IFN‐γ to renal interstitial myofibroblasts: a novel strategy for the treatment of renal fibrosis

Identification and immunohistochemical localization of macrophage migration inhibitory factor in human kidney

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