Characterization of human Rab20 overexpressed in exocrine pancreatic carcinoma

Amillet, Jean-Michel; Ferbus, Didier; Real, Francisco X.; Antony, Claude; Muleris, Martine; Gress, Thomas M.; Goubin, Gérard

doi:10.1016/j.humpath.2005.10.017

Cited by 53 publications

(46 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Transfected cells show a specific band of near the predicted mass for the fusion proteins of ∼48 kDa. of endogenous rab20 in other cell models, where rab20 localizes to the Golgi apparatus (Amillet et al 2006). By sodium dedecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblot, EGFP-rab20, had an apparent molecular weight (Mr) close to the predicted size for the fusion protein (∼48 kDa).…”

Section: Resultsmentioning

confidence: 99%

“…rab20 is highly expressed in a number of epithelia, including kidney, intestine, and lung (Lutcke et al 1994;McMurtrie et al 1997). Intriguingly, increased rab20 expression was found in 12 out of 21 patients with a class of preleukemic disorders known as myelodysplastic syndromes (Pellagatti et al 2004) and in 15 of 18 exocrine pancreatic adenocarcinomas (Amillet et al 2006), suggesting a role for rab20 in tumor progression. The ability of rab20 to interfere with formation of gap junctions by Cx43 fits well with the association of decreased Cx43 activity with cancer (Goldberg et al 2000;Shao et al 2005).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of rab20 as a Potential Regulator of Connexin43 Trafficking

Sarma

Kaplan

Willemsen

et al. 2008

Cell Communication & Adhesion

View full text Add to dashboard Cite

Connexin oligomerization and trafficking are regulated processes. To identify proteins that control connexin43 (Cx43), a screen was designed using HeLa cells expressing a Cx43 construct with di-lysine endoplasmic reticulum (ER)-retention/retrieval motif, Cx43-HKKSL. At moderate levels of expression, Cx43-HKKSL is retained in the ER as monomers; however, Cx43-HKKSL stably overexpressed by HeLa cells localizes to the perinuclear region and oligomerizes. HeLa/Cx43-HKKSL overexpressors were transiently transfected with pooled clones from a human kidney cDNA library and used immunofluorescence microscopy to identify cDNAs that enabled overexpressed Cx43-HKKSL to convert from a perinuclear to ER localization pattern. Using this approach, a small molecular weight GTPase, rab20, was identified as a candidate protein with the ability to regulate Cx43 trafficking. Enhanced green florescent protein (EGFP)-tagged rab20 showed a predominantly perinuclear and ER localization pattern and caused wild-type Cx43 to be retained inside the cell. By contrast, mutant EGFP-rab20T19N, which lacks the ability to bind GTP, had no effect on Cx43. These results suggest Cx43 is transported through an intracellular compartment regulated by rab20 along the secretory pathway.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification of rab20 as a Potential Regulator of Connexin43 Trafficking

Sarma

Kaplan

Willemsen

et al. 2008

Cell Communication & Adhesion

View full text Add to dashboard Cite

show abstract

“…However, several Rabs were found only in one type of LRO; for example, Rabs 7L1, 20, 27A, 34, 39B and 41 were identified only in melanosomes. From this group, Rab7L1 [25], 2004), Rab-20 and Rab-34 are associated with trafficking from the Golgi [26,27]. Rab34 interacts with Rab7 [28] and Rab20 is associated with the polarized sorting of V-ATPase, a critical component of the pH regulating machinery in melanosomes.…”

Section: Proteins Shared Among Lros-amongmentioning

confidence: 99%

Comparative bioinformatics analyses and profiling of lysosome-related organelle proteomes

Valencia

Huang

et al. 2007

International Journal of Mass Spectrometry

View full text Add to dashboard Cite

Complete and accurate profiling of cellular organelle proteomes, while challenging, is important for the understanding of detailed cellular processes at the organelle level. Mass spectrometry technologies coupled with bioinformatics analysis provide an effective approach for protein identification and functional interpretation of organelle proteomes. In this study, we have compiled human organelle reference datasets from large-scale proteomic studies and protein databases for 7 lysosome-related organelles (LROs), as well as the endoplasmic reticulum and mitochondria, for comparative organelle proteome analysis. Heterogeneous sources of human organelle proteins and rodent homologs are mapped to human UniProtKB protein entries based on ID and/or peptide mappings, followed by functional annotation and categorization using the iProXpress proteomic expression analysis system. Cataloging organelle proteomes allows close examination of both shared and unique proteins among various LROs and reveals their functional relevance. The proteomic comparisons show that LROs are a closely related family of organelles. The shared proteins indicate the dynamic and hybrid nature of LROs, while the unique transmembrane proteins may represent additional candidate marker proteins for LROs. This comparative analysis, therefore, provides a basis for hypothesis formulation and experimental validation of organelle proteins and their functional roles.

show abstract

“…However, the incidence of breast cancer may also be associated with other unknown genes, and studies have suggested that the tumorigenesis and metastasis of certain cancer types is strongly associated with the loss of Rab gene function (10)(11)(12). The Rab family is the largest subfamily of the small GTP-binding protein Ras superfamily, which plays a regulatory role as a GTP-dependent molecular switch at different stages of vesicular transport (13).…”

Section: Discussionmentioning

confidence: 99%

Expression of WTH3 in breast cancer tissue and the effects on the biological behavior of breast cancer cells

Gan

Zuo

Wang

et al. 2015

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. The aim of the present study was to investigate the expression of WTH3 in tumor and normal breast tissue. The mRNA and protein expression levels of WTH3 were detected using reverse transcription quantitative polymerase chain reaction and western blot analysis, respectively. In addition, matrix metalloproteinase (MMP)-2 protein expression was measured. The effect of WTH3 expression on the proliferation activity of breast cancer cells was detected using a Cell Counting Kit-8 assay. Furthermore, the effects of WTH3 on the invasion and migration ability of the breast cancer cells was investigated. The results revealed that WTH3 was able to significantly inhibit the proliferation of the MCF-7 and MDA-MB-231 breast cancer cell lines. In addition, the invasion and migration assay demonstrated that WTH3 was able to inhibit the invasion and migration of breast cancer cells. Western blot analysis revealed that increased expression of WTH3 resulted in decreased expression levels of MMP-2, which has an important function in the metastasis of cancer cells. In conclusion, WTH3 expression differed between the tumor and normal breast tissues. WTH3 was able to inhibit the proliferation of breast cancer cells and decrease their invasion ability. Thus, WTH3 may be a promising target for breast cancer therapy in the future.

show abstract

Characterization of human Rab20 overexpressed in exocrine pancreatic carcinoma

Cited by 53 publications

References 22 publications

Identification of rab20 as a Potential Regulator of Connexin43 Trafficking

Identification of rab20 as a Potential Regulator of Connexin43 Trafficking

Comparative bioinformatics analyses and profiling of lysosome-related organelle proteomes

Expression of WTH3 in breast cancer tissue and the effects on the biological behavior of breast cancer cells

Contact Info

Product

Resources

About