Characterization of human papillomavirus 6b L1 virus-like particles isolated from silkworms using capillary zone electrophoresis

Hahne, Thomas; Palaniyandi, Muthukutty; Kato, Tatsuya; Fleischmann, Peter; Wätzig, Hermann; Park, Enoch Y.

doi:10.1016/j.jbiosc.2014.02.026

Cited by 7 publications

(4 citation statements)

References 26 publications

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“…However, two excellent reviews describe the possible conditions for the characterization of viruses in general [85,86]. Based on Kremser et al [85], methods to characterize rhinovirus subviral A particles and papillomavirus 6b L1 VLPs have been developed, using slightly alkaline borate buffers containing the tenside Thesit® [87,88]. Furthermore, two following recent articles are highly recommended for an overview of this field, even though they deal with adenoviruses, which are quite different to Coronaviridae [89,90].…”

Section: Electrophoresismentioning

confidence: 99%

Physicochemical properties of SARS‐CoV‐2 for drug targeting, virus inactivation and attenuation, vaccine formulation and quality control

et al. 2020

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The material properties of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its proteins are discussed. We review the viral structure, size, rigidity, lipophilicity, isoelectric point, buoyant density and centrifugation conditions, stability against pH, temperature, UV light, gamma radiation, and susceptibility to various chemical agents including solvents and detergents. Possible inactivation, downstream, and formulation conditions are given including suitable buffers and some first ideas for quality-control methods. This information supports vaccine development and discussion with competent authorities during vaccine approval and is certainly related to drug-targeting strategies and hygienics. Several instructive tables are given, including the pI and grand average of hydropathicity (GRAVY) of SARS-CoV-1 and-2 proteins in comparison. SARS-CoV-1 and SARS-CoV-2 are similar in many regards, so information can often be derived. Both are unusually stable, but sensitive at their lipophilic membranes. However, since seemingly small differences can have strong effects, for example, on immunologically relevant epitope settings, unevaluated knowledge transfer from SARS-CoV-1 to SARS-CoV-2 cannot be advised. Published knowledge regarding downstream processes, formulations and quality assuring methods is, as yet, limited. However, standard approaches employed for other viruses and vaccines seem to be feasible including virus inactivation, centrifugation conditions, and the use of adjuvants.

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Section: Electrophoresismentioning

confidence: 99%

Physicochemical properties of SARS‐CoV‐2 for drug targeting, virus inactivation and attenuation, vaccine formulation and quality control

et al. 2020

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“…CE analysis offers many advantages such as online monitoring, rapid detection, and low consumption of sample and reagents. CZE, the most popular CE for SLPPs analysis, has been regarded to be a potential option for the quality control of SLPPs in R&D of pharmaceuticals [49].…”

Section: Analytical Techniques For Rapid Characterization and Quantifmentioning

confidence: 99%

Characterization and stabilization in process development and product formulation for super large proteinaceous particles

Yang

et al. 2020

Engineering in Life Sciences

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Super large proteinaceous particles (SLPPs) such as virus, virus like particles, and extracellular vesicles have successful and promising applications in vaccination, gene therapy, and cancer treatment. The unstable nature, the complex particulate structure and composition are challenges for their manufacturing and applications. Rational design of the processing should be built on the basis of fully understanding the characteristics of these bio‐particles. This review highlights useful analytical techniques for characterization and stabilization of SLPPs in the process development and product formulations, including high performance size exclusion chromatography, multi‐angle laser light scattering, asymmetrical flow field‐flow fractionation, nanoparticle tracking analysis, CZE, differential scanning calorimetry, differential scanning fluorescence, isothermal titration calorimetry , and dual polarization interferometry. These advanced analytical techniques will be helpful in obtaining deep insight into the mechanism related to denaturation of SLPPs, and more importantly, in seeking solutions to preserve their biological functions against deactivation or denaturation. Combination of different physicochemical techniques, and correlation with in vitro or in vivo biological activity analyses, are considered to be the future trend of development in order to guarantee a high quality, safety, and efficacy of SLPPs.

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“…Consequently, non‐enveloped icosahedral viruses possess a global negative surface charge at pH higher than the isoelectric point contributing to proffer them an electrophoretic mobility . Exploiting this characteristic, it has been shown that capillary zone electrophoresis (CZE), a separative analytical technique based on the difference of the electrophoretic mobility of the analytes , can be applied in the separation, detection and quantification of viral, subviral and virus‐like particles in aqueous media . However, in practice, the CZE analysis of viruses is harder than of molecules, where uncontrolled viral particles aggregation and capillary wall adsorption responsible of irreproducible results having been often encountered .…”

Section: Introductionmentioning

confidence: 99%

MS2 and Qβ bacteriophages reveal the contribution of surface hydrophobicity on the mobility of non‐enveloped icosahedral viruses in SDS‐based capillary zone electrophoresis

et al. 2017

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SDS is commonly employed as BGE additive in CZE analysis of non-enveloped icosahedral viruses. But the way by which SDS interacts with the surface of such viruses remains to date poorly known, making complicate to understand their behavior during a run. In this article, two related bacteriophages, MS2 and Qβ, are used as model to investigate the migration mechanism of non-enveloped icosahedral viruses in SDS-based CZE. Both phages are characterized by similar size and surface charge but significantly different surface hydrophobicity (Qβ > MS2, where '>' means 'more hydrophobic than'). By comparing their electrophoretic mobility in the presence or not of SDS on both sides of the CMC, we show that surface hydrophobicity of phages is a key factor influencing their mobility and that SDS-virus association is driven by hydrophobic interactions at the surface of virions. The CZE analyses of heated MS2 particles, which over-express hydrophobic domains at their surface, confirm this finding. The correlations between the present results and others from the literature suggest that the proposed mechanism might not be exclusive to the bacteriophages examined here.

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Characterization of human papillomavirus 6b L1 virus-like particles isolated from silkworms using capillary zone electrophoresis

Cited by 7 publications

References 26 publications

Physicochemical properties of SARS‐CoV‐2 for drug targeting, virus inactivation and attenuation, vaccine formulation and quality control

Physicochemical properties of SARS‐CoV‐2 for drug targeting, virus inactivation and attenuation, vaccine formulation and quality control

Characterization and stabilization in process development and product formulation for super large proteinaceous particles

MS2 and Qβ bacteriophages reveal the contribution of surface hydrophobicity on the mobility of non‐enveloped icosahedral viruses in SDS‐based capillary zone electrophoresis

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