Characterization of human ovarian and endometrial carcinoma cell lines established on extracellular matrix

Crickard, Kent; Niedbala, Michael J.; Crickard, Ulla; Yoonessi, Mahmood; Sandberg, Avery A.; Okuyama, Kazuhiko; Bernacki, Ralph J.; Satchidanand, Sateesh

doi:10.1016/s0090-8258(89)80028-9

Cited by 33 publications

(16 citation statements)

References 13 publications

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“…Most human ovarian cancer cell lines described were derived from ascites or pleural e usion (Alama et al, 1996;Bast et al, 1981;Buller et al, 1995;DiSaia et al, 1975;Fogh and Trempe, 1975;Golombick and Bezwoda, 1991;Golombick et al, 1990;Grunt et al, 1993;Hamilton et al, 1983;Hirte et al, 1994;Langdon et al, 1988;Provencher et al, 1993;Sinna et al, 1979;Wong et al, 1990;Yamada et al, 1991), with only a few cell lines derived from primary ovarian solid tumors (Crickard et al, 1989;Langdon et al, 1988;Woods et al, 1979) or metastases (Buick et al, 1985). In addition, most cell lines originate from tumor material obtained following adjuvant therapy such as chemotherapy or radiation therapy, which could introduce confounding genetic events.…”

Section: Discussionmentioning

confidence: 99%

Microarray analysis of gene expression mirrors the biology of an ovarian cancer model

et al. 2001

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Section: Discussionmentioning

confidence: 99%

Microarray analysis of gene expression mirrors the biology of an ovarian cancer model

et al. 2001

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“…The A2780/DX5B was cloned front the A2780/DX5 line. The human ovarian cell line, A121, was established by Crickard et al (1989). Both parental and resistant A2780 cells were grown in RPMI-1640 medium supplemented with 1% glutamine and 10% fetal calf serum.…”

Section: Materiais and Metf-fodsmentioning

confidence: 99%

Parabolic growth patterns in 96-well plate cell growth experiments

Faessel

Levasseur

Slocum

et al. 1999

In Vitro Cell.Dev.Biol.-Animal

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In preparing for the routine use of the ubiquitous in vitro cell growth inhibition assay for the study of anticancer agents, we characterized the statistical properties of the assay and found some surprising results. Parabolic well-to-well cell growth patterns were discovered, which could profoundly affect the results of routine growth inhibition studies of anticancer and other agents. Four human ovarian cell lines, A2780/WT, A2780/DX5, A2780/DX5B, and A121, and one human ileocecal adenocarcinoma cell line, HCT-8, were seeded into plastic 96-well plates with a 12-channel pipette, without drugs, and grown from 1-5 d. The wells were washed with a plate washer, cells stained with sulforhodamine B (SRB), and dye absorbance measured with a plate reader. Variance models were fit to the data from replicates to determine the nature of the heteroscedastic error structure. Exponential growth models were fit to data to estimate doubling times for each cell line. Polynomial models were fit to data from 10-plate stacks of 96-well plates to explore nonuniformity of cell growth in wells in different regions of the stacks. Each separate step in the assay was examined for precision, patterns, and underlying causes of variation. Differential evaporation of water from wells is likely a major, but not exclusive, contributor to the systematic well-to-well cell growth patterns. Because the fundamental underlying causes of the parabolic growth patterns were not conclusively found, a randomization step for the growth assay was developed.

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“…Studies showed that mixed mullerian tumor cell growth is enhanced on extracellular matrix (ECM)-coated plates, relative to cells cultured on plastic alone (5)(6)(7)19). Furthermore, ECM degradation experiments indicate that metastatic potential is related to cell-cell interactions between tumor cells and supporting stromal cells (20).…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional culture of a mixed mullerian tumor of the ovary: Expression of in vivo characteristics

Goodwin

Prewett²,

Spaulding

et al. 1997

In Vitro Cell.Dev.Biol.-Animal

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The Rotating-Wall Vessel (RWV) is a novel in vitro cell culture system used to successfully culture a cell line derived from a heterologous mixed mullerian tumor cell of the ovary. Although the original tumor was comprised of both epithelial and mesodermal components, long-term culture in conventional flasks established a cell line from this tumor with homogeneous epitheliallike growth characteristics (1). Cells from Passage 36 were seeded into a Rotating-Wall Vessel containing Cytodex-3 microcarrier beads. Scanning electron micrographs of tumor cells cultured for 32 d in the RWV showed the presence of heterogeneous cell populations organized into three-dimensional tissuelike architecture. Immunocytochemical analysis confirmed the cellular heterogeneity, as demonstrated by expression of both epithelial and mesenchymal antigens. Reverse transcription polymerase chain reaction amplification demonstrated the presence of mRNA for cellular oncogenes HER-2/neu, H-ras, K-ras, and tumor suppressor p53. Thus, there are two advantages to propagation of tissue in the RWV culture system:(a) tissue diversification representing populations present in the original tumor, and (b) the three-dimensional freedom to organize tissues morphologically akin to those observed in vivo. These data indicate that the RWV culture system is suitable for generating large quantities of ovarian tumor cells in vitro that are amenable to immunocytochemical, oncogenic, morphologic characteristics demonstrated in vivo.

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Characterization of human ovarian and endometrial carcinoma cell lines established on extracellular matrix

Cited by 33 publications

References 13 publications

Microarray analysis of gene expression mirrors the biology of an ovarian cancer model

Microarray analysis of gene expression mirrors the biology of an ovarian cancer model

Parabolic growth patterns in 96-well plate cell growth experiments

Three-dimensional culture of a mixed mullerian tumor of the ovary: Expression of in vivo characteristics

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