Characterization of human extrahepatic biliary duct epithelial cells in culture

Schier, Christina J.; Schier, Felix; Voß, B.; Bassewitz, D. B. v.; Pfautsch, M.

doi:10.1016/0014-4800(88)90066-4

Cited by 13 publications

(8 citation statements)

References 8 publications

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“…Although we and others have published methods for the isolation and culture of human biliary epithelium (11,24), this study is the first step in a natural line of investigation using BEC in functional assays. We have demonstrated that the MHC molecules on the cultured BEC carry functional lymphocyte-activating determinants (i.e., they can be recognized by alloprimed T lymphocytes in a secondary proliferation assay).…”

Section: Discussionmentioning

confidence: 99%

Recognition of major histocompatibility complex antigens on cultured human biliary epithelial cells by alloreactive lymphocytes

Saidman¹

1991

Hepatology

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We have developed an in vitro system to study the interactions between biliary epithelium and lymphocytes using cultured human biliary epithelial cells. No class II antigens were detected by immunoperoxidase staining of the normal biliary epithelial cells, but alloactivated lymphocyte culture supernatants were able to induce class II expression. The activity of the supernatants was blocked with an anti-y-interferon monoclonal antibody. In addition, recombinant human y-interferon alone induced the expression of class II antigens and increased the intensity of class I staining of cultured biliary epithelial cells.· Biliary epithelial cell-induced proliferation of alloreactive T lymphocytes demonstrated that the major histocompatibility complex molecules carry functional lymphocyteactivating determinants. The recognition of major histocompatibility complex determinants was confirmed by monoclonal antibody-blocking studies and by stimulation of an alloreactive T-cell clone. However, the biliary epithelial cells were much less potent stimulators than arterial endothelial cells tested in the same assay system. (HEPATOLOGY 1991;13:239-246.)

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Section: Discussionmentioning

confidence: 99%

Recognition of major histocompatibility complex antigens on cultured human biliary epithelial cells by alloreactive lymphocytes

Saidman¹

1991

Hepatology

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“…Paradis and Sharp [35] also have recently described the development of duct-like structures in primary cultures of bile ductu lar epithelial cells isolated from mouse livers at seven to ten days after bile duct ligation when these cells were cultured under a different medium condition than ours on basement membrane Matrigel. However, it is not yet known whether intrahepatic biliary epithelial cells can form basement membrane in culture, although human extrahepatic biliary duct epithelial cells in primary cul ture were reported to show immunofluorescence for both type IV collagen and laminin [101].…”

Section: Culture and Transplantation Of Biliary Epithelial Cells And mentioning

confidence: 99%

“…Chronic administra tion of 1.0% lithocholic acid in a low-protein diet to rats produced an intrahepatic biliary hyperplasia and peri cholangitis in vivo [121], but lithocholic acid was found to be toxic to primary cultures of normal rat intrahepatic bile ducts when added at concentrations above 0.5 mM and without effect below these concentrations [99]. In addition, Glaser et al [122] have provided a preliminary characterization of an apparent protein growth sub stance present in normal rabbit and other mammalian sera, which appears to selectively stimulate the growth of extrahepatic bile ducts in genetically susceptible mice [122], Schierand Schier [100] and Schieret al [101] also reported that bovine bile duct conditioned medium con tained a substance that promoted the growth and mor phological integrity of human extrahepatic bile duct epi thelial cells in serum-free primary monolayer cell cul ture.…”

Section: Growth Properties Of Biliary Epithelial Cells and Oval Cellsmentioning

confidence: 99%

“…Pri mary monolayer cultures now have been described for normal rat and human intrahepatic biliary epithelial cells [28,32,33], for hyperplastic bile ductular epithelial cells isolated from mouse and rat livers after bile duct ligation [26,34,35], and for oval cells from the liver of rats fed hepatocarcinogenic diets [30,97,98], Short-term primary explant cultures were also developed with a hyperplastic bile ductular tissue preparation obtained from the liver of bile duct-ligated rats [26] and with intact intrahepatic bile ducts from normal rats [99], In addition, primary cultures have been established with extrahepatic biliary epithelial cells, including those iso lated from human cystic ducts [100,101] and bovine extrahepatic ducts [102], as well as cells from explants of guinea pig gallbladder mucosa [103].…”

Section: Culture and Transplantation Of Biliary Epithelial Cells And mentioning

confidence: 99%

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Isolation, Culture, and Transplantation of Intrahepatic Biliary Epithelial Cells and Oval Cells

et al. 1990

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Recent advances made in the isolation, culture, and transplantation of defined populations of intrahepatic biliary epithelial cells and oval cells have permitted direct analysis of the functions, growth properties, and differentiation potential of these respective cell types in their untransformed or transformed states. This review provides a current and comprehensive examination of the various approaches that have been taken to isolate and culture intrahepatic biliary epithelial cells from normal and cholestatic liver and oval cells from preneoplastic liver. Emphasis is placed on comparing the phenotypic features and growth properties of these various biliary cell types in vitro as well as on describing their transplantation into ectopic tissue sites. In addition, the oval cell is evaluated in terms of its potential role as a ‘facultative stem cell’ during hepatocarcinogenesis.

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“…The mechanisms involved in the pathogenesis of these diseases have not been defined so that an in vitro model of extrahepatic bile duct cell culture would be very helpful to reveal the mechanisms of these diseases at cellular level. Only a few previous studies have characterized cell cultures from either human or rodent extrahepatic bile duct structures (Schier et al 1988;Katayanagi et al 1998;Asakawa et al 2000;Hsu et al 2001;Ochiai et al 2004).…”

mentioning

confidence: 99%

A novel method for establishment and characterization of extrahepatic bile duct epithelial cells from mice

Chai

Zheng

Feng

et al. 2010

In Vitro Cell.Dev.Biol.-Animal

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Culture of extrahepatic bile duct epithelial cells is a useful model to investigate physiology of extrahepatic bile duct epithelia and hepatobiliary disease mechanisms. The aim of this work was to establish and characterize a primary murine extrahepatic bile duct epithelial cell culture. Epithelial cells were isolated from extrahepatic bile ducts of BALB/c mice that were intraperitoneally injected with newborn bovine serum to induce the proliferation of extrahepatic bile ducts' epithelial cells and cultured on rat tail type I collagen-coated plastic culture flask containing DMEM/HamF12 with 10% FBS and 10 ng/ml epidermal growth factor at 37°C in an incubator with 5% humidified CO(2). The cells showed typical morphologic characteristics of epithelial phenotypes with cobblestone appearance in monolayer within 5-6 d after culture; they were positive against anticytokeratin-19 immunostaining. Transmission electron microscopy showed typical bile duct epithelia with microvilli on the cytomembrane, Golgi complex, massive mitochondria, and rough endoplasmic reticulum in the cytoplasmic. The growth curve of the epithelial cells was determined by a MTT assay which showed a normal sigmoidal growth curve. This culture technique might be a reliable method for isolation, purification, and primary culture of extrahepatic bile duct epithelial cells that can serve as a model for in vitro studies on the pathophysiology of hepatobiliary diseases as well as pharmacological and toxicological targets relevant to hepatobiliary diseases.

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Characterization of human extrahepatic biliary duct epithelial cells in culture

Cited by 13 publications

References 8 publications

Recognition of major histocompatibility complex antigens on cultured human biliary epithelial cells by alloreactive lymphocytes

Recognition of major histocompatibility complex antigens on cultured human biliary epithelial cells by alloreactive lymphocytes

Isolation, Culture, and Transplantation of Intrahepatic Biliary Epithelial Cells and Oval Cells

A novel method for establishment and characterization of extrahepatic bile duct epithelial cells from mice

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