Characterization of hospital and community‐acquired respiratory syncytial virus in children with severe lower respiratory tract infections in Ho Chi Minh City, Vietnam, 2010

Tuấn, Trần Anh; Thanh, Tran Tan; Hai, Nguyen Thi Thanh; Tinh, Le Binh Bao; Kim, Le thi Ngoc; Ha, Lien Anh; B’Krong, Nguyen Thi Thuy Chinh; Thắm, Nguyễn Thị; Hang, Vu Thi Ty; Merson, Laura; Farrar, Jeremy; Thuong, Tang Chi; Jong, Menno D. de; Schultsz, Constance; Doorn, H. Rogier van

doi:10.1111/irv.12307

Cited by 12 publications

(16 citation statements)

References 22 publications

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“…Five NA-1 nucleotide sequences of this study had a premature stop codon (Q298Stop) that resulted in a sequence of 64 amino acids. The substitutions N237D, S269T, P274L, P286L, P289S, P292S, R297K, and Q298stop, previously reported as specific to NA-1 genotype [ 5 , 12 , 21 , 35 , 40 ], were also found in the NA-1 sequences of the current study. The HVR2 of 38 BA-9 strains of the present study were aligned with sequences from other countries and the BA4128/99B reference sequence (Fig.…”

Section: Resultssupporting

confidence: 83%

Genetic diversity of human respiratory syncytial virus isolated among children with acute respiratory infections in Southern Cameroon during three consecutive epidemic seasons, 2011–2013

et al. 2018

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BackgroundHuman respiratory syncytial virus (HRSV) is the main viral cause of severe lower respiratory tract disease in infants and young children. The aim of this study was to describe for the first time the genetic variability of HRSV in Cameroonian patients living in Yaounde for three consecutive epidemic seasons.MethodsHRSV-positive nasopharyngeal samples detected in children less than 15 years in Yaounde were collected from September 2011 to December 2013. Semi-nested RT-PCR, sequencing, and phylogenetic analyses of the second hypervariable region of the G gene were performed.ResultsA total of 57 HRSV-positive samples were collected during the study period. Among these, 46 (80.7%) could be amplified in the G gene. HRSV group A (HRSV-A) and group B (HRSV-B) co-circulated in this population at 17.4 and 82.6%, respectively. HRSV-A strains clustered in the NA-1 genotype while HRSV-B strains clustered in the BA-9 genotype. HRSV-A strains accounted for 33.3% (2/6), 4.3% (1/23), and 29.4% (5/17) of the viruses isolated in 2011, 2012, and 2013, respectively.ConclusionsThis study reports molecular epidemiology data of HRSV in Cameroon for the first time. Additional studies are required to clarify evolutionary patterns of HRSV throughout sub-Saharan Africa to support antiviral and vaccine development.

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Section: Resultssupporting

confidence: 83%

Genetic diversity of human respiratory syncytial virus isolated among children with acute respiratory infections in Southern Cameroon during three consecutive epidemic seasons, 2011–2013

et al. 2018

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“…In this study, RSV strains circulating in Lao PDR were characterized for the first time, with the detection of the common genotypes of RSV‐A NA1, RSV‐B BA9, and the rare CB1/THB genotype. Cocirculation of different genotypes in a country during RSV season is not uncommon . Most RSV genotypes are often shared between various regions of the world.…”

Section: Discussionmentioning

confidence: 99%

Etiology of viral respiratory infections in Northern Lao People's Democratic Republic

Snoeck

Ponghsavath²,

Luetteke

et al. 2018

Journal of Medical Virology

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In Lao People's Democratic Republic (PDR), acute respiratory infections overburden the health care system, but viral etiology, genetic diversity, and seasonality, especially in light of the introduction of influenza vaccination in the country, are poorly understood. From August 2010 to April 2011, 309 outpatients were recruited at the Luang Prabang Provincial Hospital covering highland Lao communities. Nasopharyngeal swabs were screened for the presence of 13 respiratory viruses. At least one virus was detected in 69.6% and dual/triple viral infections in 12.9%/1.9% of the patients. Influenza A and B viruses combined were the most frequently detected pathogens, followed by human adenovirus and respiratory syncytial virus (RSV). The other viruses were detected in less than 10% of the patients. Phylogenetic analyses on a representative set of RSV strains revealed that, while otherwise very rare, the RSV-B CB1/THB genotype cocirculated with other common genotypes. A single wave of influenza virus and RSV activity was observed during the rainy season, providing further support to influenza vaccination before the onset of the rains. This study provides recommendations for influenza vaccination that still needs optimization and highlights the need for revised guidelines for treatment and prevention of respiratory infections in Lao PDR, as well as for increased surveillance efforts.

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“…BA9 appeared in Thailand beginning in 2010 and is now the predominant genotype, while other RSV-B genotypes appeared only sporadically. BA9 and BA10 are commonly identified in Southeast Asia ( Arnott et al, 2011 ; Tran et al, 2013 ; Tuan et al, 2015 ). It is noteworthy that the BA genotypes, like the ON1, only recently emerged to replace all other RSV-B strains ( Trento et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Respiratory syncytial virus genotypes NA1, ON1, and BA9 are prevalent in Thailand, 2012–2015

Thongpan

Mauleekoonphairoj

Vichiwattana

et al. 2017

PeerJ

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Respiratory syncytial virus (RSV) causes acute lower respiratory tract infection in infants and young children worldwide. To investigate the RSV burden in Thailand over four consecutive years (January 2012 to December 2015), we screened 3,306 samples obtained from children ≤5 years old with acute respiratory tract infection using semi-nested reverse-transcription polymerase chain reaction (RT-PCR). In all, 8.4% (277/3,306) of the specimens tested positive for RSV, most of which appeared in the rainy months of July to November. We then genotyped RSV by sequencing the G glycoprotein gene and performed phylogenetic analysis to determine the RSV antigenic subgroup. The majority (57.4%, 159/277) of the RSV belonged to subgroup A (RSV-A), of which NA1 genotype was the most common in 2012 while ON1 genotype became prevalent the following year. Among samples tested positive for RSV-B subgroup B (RSV-B) (42.6%, 118/277), most were genotype BA9 (92.6%, 87/94) with some BA10 and BA-C. Predicted amino acid sequence from the partial G region showed highly conserved N-linked glycosylation site at residue N237 among all RSV-A ON1 strains (68/68), and at residues N296 (86/87) and N310 (87/87) among RSV-B BA9 strains. Positive selection of key residues combined with notable sequence variations on the G gene contributed to the continued circulation of this rapidly evolving virus.

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Characterization of hospital and community‐acquired respiratory syncytial virus in children with severe lower respiratory tract infections in Ho Chi Minh City, Vietnam, 2010

Cited by 12 publications

References 22 publications

Genetic diversity of human respiratory syncytial virus isolated among children with acute respiratory infections in Southern Cameroon during three consecutive epidemic seasons, 2011–2013

Genetic diversity of human respiratory syncytial virus isolated among children with acute respiratory infections in Southern Cameroon during three consecutive epidemic seasons, 2011–2013

Etiology of viral respiratory infections in Northern Lao People's Democratic Republic

Respiratory syncytial virus genotypes NA1, ON1, and BA9 are prevalent in Thailand, 2012–2015

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