Characterization of Hormone-Dependent Pathways in Six Human Prostate-Cancer Cell Lines: A Gene-Expression Study

Abstract

Prostate cancer (PCa), the most incident cancer in men, is tightly regulated by endocrine signals. A number of different PCa cell lines are commonly used for in vitro experiments, but these are of diverse origin, and have very different cell-proliferation rates and hormone-response capacities. By analyzing the gene-expression pattern of main hormone pathways, we systematically compared six PCa cell lines and parental primary cells. We compared these cell lines (i) with each other and (ii) with PCa tissue samples from 11 patients. We found major differences in the gene-expression levels of androgen, insulin, estrogen, and oxysterol signaling between PCa tissue and cell lines, and between different cell lines. Our systematic characterization gives researchers a solid basis to choose the appropriate PCa cell model for the hormone pathway of interest.

miR ‐27 and miR ‐124 target AR coregulators in prostate cancer: Bioinformatics and in vitro analysis

“…Franco et al reported that differences in expression of genes in six prostate cancer cell lines were accounted for by hormone‐dependent cell pathways, including the AR pathway (Franko et al, 2020). miR‐27a‐3p was posited to be a tumour suppressor or an oncogene in multiple cancers such hepatocellular carcinoma, pancreatic, bladder, colon and breast cancers (Li et al, 2019).…”

Increased Expressions of Matrix Metalloproteinases (MMPs) in Prostate Cancer Tissues of Men with Type 2 Diabetes

“…The human prostate adenocarcinoma cell line PC3 was purchased from CLS-Cell line services GmbH (Eppelheim, Germany). Cells were maintained in low glucose (5.5 mM) DMEM (Gibco/Thermo Fisher Scientific, Karlsruhe, Germany) supplemented with 5% fetal bovine serum (FBS) (Bio&Sell, Feucht, Germany) [ 19 ]. One day before treatment, medium was changed to DMEM with 0.2% FBS.…”