Characterization of HIV-1 genetic diversity and antiretroviral resistance in the state of Maranhão, Northeast Brazil

Abstract

The HIV-1 epidemic in Brazil has been growing in northeast and north regions, particularly an increase in AIDS cases among the younger male population has been observed. This study aims to characterize the HIV-1 genetic diversity and to evaluate its antiretroviral resistance profile among individuals presenting virological failure in the state of Maranhão-Brazil. HIV-1 pol gene sequences from 633 patients on antiretroviral therapy were obtained from the Department of Surveillance, Prevention and Control of Sexually Transmitted Infections, HIV/AIDS and Viral Hepatitis of the Brazilian Ministry of Health. Phylogenetic and recombination analyses were performed to characterize viral genetic diversity. The presence of antiretroviral resistance mutations was assessed using the HIV Drug Resistance Database online platform of Stanford University. A predominance of subtype B (84.5%) was observed, followed by recombinant BF (9.5%), where more than half of the sequences were dispersed in 3 clusters. Antiretroviral resistance was detected in 74.1% of the sequences, and it was significantly higher for nucleoside analogue reverse-transcriptase inhibitors (NRTIs) than for non-nucleoside analogue reverse-transcriptase inhibitors (NNRTIs) and protease inhibitors (PIs). Inference of putative transmissions clusters identified 11 clusters with 22 query sequences (22/633, 3.5%). Thus, we conclude that continuous monitoring of the molecular epidemiology of HIV-1 is essential for prevention strategies, epidemic control, and treatment adequacy.

Mutações de resistência a antirretrovirais e diversidade genética do HIV-1 em gestantes atendidas em uma maternidade de referência no Estado do Piauí, Brasil

“…Os achados deste estudo corroboram essa teoria e indicam claramente que a maternidade de referência em Teresina atende pessoas infectadas com HIV-1 oriundas de municípios circunvizinhos, inclusive do estado do Maranhão. Neste estado brasileiro vizinho ao Piauí também há predominância do subtipo B do HIV-1 (Leal et al 2020). Apesar dos relatos de existência dos subtipos puros C, F1 e D e diversos recombinantes no Brasil, o reduzido fluxo de pessoas de outras regiões do Brasil e de outros países pode estar diminuindo a introdução de subtipos não-B e os possíveis recombinantes.…”