Characterization of highly purified, inactivated HIV-1 particles isolated by anion exchange chromatography

Richieri, Steven P.; Bartholomew, Richard M.; Aloia, Roland C.; Savary, Jay R.; Gore, Richard; Holt, John Clifford; Ferre, Francois; Musil, Roy; Tian, Hua; Trauger, Richard; Lowry, Peter; Jensen, Fred C.; Carlo, Dennis J.; Maigetter, Robert Z.; Prior, Christopher P.

doi:10.1016/s0264-410x(97)00196-5

Cited by 45 publications

(32 citation statements)

References 11 publications

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“…Anion exchange chromatography has been previously used to purify inactivated HIV-1 particles for immune-based therapy purposes. 16 These particles were found to be of high purity (4 95%) as estimated by analytical gel filtration and thin section electron microscopy. 17 In our study, we demonstrate that anion exchange chromatography can also be used for the concentration of infectious, VSV-G pseudotyped lentiviral vector particles without major loss in infectivity.…”

Section: Discussionmentioning

confidence: 91%

Efficient gene transfer into the CNS by lentiviral vectors purified by anion exchange chromatography

et al. 2002

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Section: Discussionmentioning

confidence: 91%

Efficient gene transfer into the CNS by lentiviral vectors purified by anion exchange chromatography

et al. 2002

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“…delivery and protection against IVAG virus challenge in a mouse model. The HIV-1 Ag used here was an early isolate from an HIV-1-infected individual in Zaire in 1976 (HZ321) that contains a clade A envelope and clade G gag (5,37). Our results show that i.n.…”

mentioning

confidence: 76%

CD8⁺T-Cell-Mediated Cross-Clade Protection in the Genital Tract following Intranasal Immunization with Inactivated Human Immunodeficiency Virus Antigen Plus CpG Oligodeoxynucleotides

Jiang

Patrick

Moss

et al. 2005

J Virol

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Human immunodeficiency virus (HIV) is a mucosally transmitted infection that rapidly targets and depletes CD4؉ T cells in mucosal tissues and establishes a major reservoir for viral persistence in gut-associated lymphoid tissues. Therefore, vaccines designed to prevent HIV infections must induce potent and durable mucosal immune responses, especially in the genital tract. Here we investigated whether intranasal (i.n.) immunization with inactivated gp120-depleted HIV-1 antigen (

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“…One of the critical yet unresolved issues is from what cellular-or viralcompartment Vif exerts its activity. Even though previous studies have clearly identified Vif within HIV virions (10,21,34,39,49), its functional relevance has been questioned. Unlike Vpr, which is packaged into HIV particles through an interaction with the p6 component of Gag (12,36,37,47,71), packaging of Vif was thought to be nonspecific (10,56).…”

Section: Discussionmentioning

confidence: 99%

Human Immunodeficiency Virus Type 1 Vif Protein Is Packaged into the Nucleoprotein Complex through an Interaction with Viral Genomic RNA

Khan

Aberham

Kao

et al. 2001

J Virol

127

144

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The human immunodeficiency virus type 1 (HIV-1) Vif protein plays a critical role in the production of infectious virions. Previous studies have demonstrated the presence of small amounts of Vif in virus particles. However, Vif packaging was assumed to be nonspecific, and its functional significance has been questioned. We now report that packaging of Vif is dependent on the packaging of viral genomic RNA in both permissive and restrictive HIV-1 target cells. Mutations in the nucleocapsid zinc finger domains that abrogate packaging of viral genomic RNA abolished packaging of Vif. Additionally, an RNA packaging-defective virus exhibited significantly reduced packaging of Vif. Finally, deletion of a putative RNA-interacting domain in Vif abolished packaging of Vif into virions. Virion-associated Vif was resistant to detergent extraction and copurified with components of the viral nucleoprotein complex and functional reverse transcription complexes. Thus, Vif is specifically packaged into virions as a component of the viral nucleoprotein complex. Our data suggest that the specific association of Vif with the viral nucleoprotein complex might be functionally significant and could be a critical requirement for infectivity of viruses produced from restrictive host cells.

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Characterization of highly purified, inactivated HIV-1 particles isolated by anion exchange chromatography

Cited by 45 publications

References 11 publications

Efficient gene transfer into the CNS by lentiviral vectors purified by anion exchange chromatography

Efficient gene transfer into the CNS by lentiviral vectors purified by anion exchange chromatography

CD8⁺T-Cell-Mediated Cross-Clade Protection in the Genital Tract following Intranasal Immunization with Inactivated Human Immunodeficiency Virus Antigen Plus CpG Oligodeoxynucleotides

Human Immunodeficiency Virus Type 1 Vif Protein Is Packaged into the Nucleoprotein Complex through an Interaction with Viral Genomic RNA

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Characterization of highly purified, inactivated HIV-1 particles isolated by anion exchange chromatography

Cited by 45 publications

References 11 publications

Efficient gene transfer into the CNS by lentiviral vectors purified by anion exchange chromatography

Efficient gene transfer into the CNS by lentiviral vectors purified by anion exchange chromatography

CD8+T-Cell-Mediated Cross-Clade Protection in the Genital Tract following Intranasal Immunization with Inactivated Human Immunodeficiency Virus Antigen Plus CpG Oligodeoxynucleotides

Human Immunodeficiency Virus Type 1 Vif Protein Is Packaged into the Nucleoprotein Complex through an Interaction with Viral Genomic RNA

Contact Info

Product

Resources

About

CD8⁺T-Cell-Mediated Cross-Clade Protection in the Genital Tract following Intranasal Immunization with Inactivated Human Immunodeficiency Virus Antigen Plus CpG Oligodeoxynucleotides