Characterization of Highly Prevalent Plasmids Coharboringmcr-1,oqxAB, andblaCTX-Mand Plasmids HarboringoqxABandblaCTX-MinEscherichia coliIsolates from Food-Producing Animals in China

Liu, Bao‐Tao; Song, Feng-Jing; Zou, Ming

doi:10.1089/mdr.2017.0391

Cited by 14 publications

(3 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Conjugation was performed and all bla NDM-5 -carrying plasmids could be transferred by conjugation to the recipient E. coli C600, whereas no bla KPC-2 -positive transconjugants were obtained. The transconjugants confirmed as we previously described (Liu, Song, & Zou, 2019) only exhibited high-level resistance to all tested beta-lactams and the MICs of meropenem were up to ≥32 mg/L (Table 1).…”

Section: Re Sults and Discussionsupporting

confidence: 80%

Occurrence and characterization of KPC‐2‐producing ST11 Klebsiella pneumoniae isolate and NDM‐5‐producing Escherichia coli isolate from the same horse of equestrian clubs in China

Wang

Liu

2020

Transbounding Emerging Dis

View full text Add to dashboard Cite

Carbapenems are an important last resort to treat clinically important multidrug-resistant gram-negative bacteria. However, the populations of carbapenem-resistant Enterobacteriaceae (CRE) have been rapidly increasing not only in patients (Zhang et al., 2017) but also in the community (Kelly, Mathema, & Larson, 2017) worldwide in recent years, posing a serious threat to public health. The main mechanism of carbapenem resistance within Enterobacteriaceae is the production of carbapenemases, frequently encoded on transmissible plasmids. The major three types of carbapenemases are the New Delhi metallo-β-lactamase (NDM), Klebsiella pneumoniae carbapenemase (KPC) and OXA-48-type oxacillinase (Kelly et al., 2017). Besides humans (Hsu et al., 2017), NDMproducing Enterobacteriaceae isolates, especially Escherichia coli, have

show abstract

Section: Re Sults and Discussionsupporting

confidence: 80%

Occurrence and characterization of KPC‐2‐producing ST11 Klebsiella pneumoniae isolate and NDM‐5‐producing Escherichia coli isolate from the same horse of equestrian clubs in China

Wang

Liu

2020

Transbounding Emerging Dis

View full text Add to dashboard Cite

show abstract

“…IncHI2/HI2A plasmids are typically large ( García-Fernández and Carattoli, 2010 ), multidrug-resistant plasmids that have been accompanied by a range of antimicrobial and metal resistance genes in Salmonella species from humans and food-producing animals ( Linxian et al, 2017 ; Elnekave et al, 2018 ; Mather et al, 2018 ; Paudyal et al, 2018 ; Biswas et al, 2019 ; Elbediwi et al, 2019 ; Lu et al, 2019 ; Monte et al, 2019 ). The presence of IncHI2 plasmids in Salmonella serovars indicates that horizontal transfer of mcr-1 -harboring plasmids might have also contributed to the spread of mcr-1 and other resistant determinants in these bacteria ( Liu et al, 2018 ). IncHI2 plasmids also carried a diversity of antimicrobial resistance genes from different categories, including aminoglycosides, beta-lactams, tetracycline, sulfonamides, and phenicols.…”

Section: Discussionmentioning

confidence: 99%

Genomic Characterization of mcr-1-carrying Salmonella enterica Serovar 4,[5],12:i:- ST 34 Clone Isolated From Pigs in China

Elbediwi

Pan

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Salmonella enterica serovar 4,[5],12:i:-, so-called Typhimurium monophasic variant, has become one of the most frequently isolated serovars both in humans and in animals all over the world. The increasing prevalence of mcr-1-carrying Salmonella poses significant global health concerns. However, the potential role of Salmonella 4,[5],12:i:-in mcr-1 gene migration through the food chain to the human remains obscure. Here, we investigated 337 Salmonella isolates from apparently healthy finishing pigs, which is rarely studied, obtained from pig farms and slaughterhouses in China. The mcr-1 gene was found in four colistin-resistant S. enterica 4,[5],12:i:-isolates. Notably, all four isolates belonged to sequence type 34 (ST34) with multidrug resistance phenotype. Further genomic sequencing and antimicrobial resistance characterization confirmed that mcr was responsible for the colistin resistance, and the conjugation assay demonstrated that three of four isolates carried mcr-1 in IncHI2 plasmid. Importantly, mcr-1 and class-1 integron were found to co-localize in two strains with IncHI2 plasmid. By collecting all the mcr-1-carrying Typhimurium and monophasic variant strains across the food chain (farm animals, animal-origin food, and humans), our phylogenomic analysis of available 66 genomes, including four strains in this study, demonstrated an independent phylogenetic cluster of all eight Chinese swine-originated isolates and one human isolate. Together, this study provides direct evidence for clonal and pork-borne transmission of mcr-1 by Salmonella 4,[5],12:i:-ST34 in China and highlighted a domestication pathway by acquisition of additional antimicrobial resistance determinants in Chinese ST34 isolates.

show abstract

“…Interestingly, none of the patients received previous treatment with colistin or had close contact with farm animals. We postulate that considering the occurrence of mcr-1 -positive Enterobacteriaceae in the environment, vegetables, and meat in China,28–30 the patients might have been exposed to the mcr-1 gene via these sources. Similar to previous reports,7,17,18,27 all three patients with mcr-1 -positive E. coli bloodstream infection had a favorable outcome after treatment with antibacterials that they had been found to be susceptible to.…”

Section: Discussionmentioning

confidence: 99%

Epidemiology and molecular characterization of mcr-1 in Escherichia coli recovered from patients with bloodstream infections in Changsha, central China

Zhong¹,

Liu²,

Zheng³

2019

IDR

View full text Add to dashboard Cite

Objectives The main aim of this study was to investigate the prevalence and molecular characteristics of the mcr-1 gene in Escherichia coli isolates obtained from all patients with bloodstream infections over a year in a Chinese teaching hospital. We also assessed the susceptibility profiles of the mcr-1 -positive strains and prognostic impact of this gene on the patients. Methods A total of 144 consecutive, non-repetitive E. coli isolates causing bloodstream infections were collected at a teaching hospital in Changsha, China from January to December 2016. The presence of the mcr-1 gene was assessed by PCR. All mcr-1 -positive E coli isolates were characterized by antimicrobial susceptibility testing, multilocus sequence typing (MLST), a conjugation experiment, and plasmid replicon typing. Clinical data were obtained from medical records. Results The mcr-1 gene was detected in three (2.1%) of the 144 E. coli isolates. The three mcr-1 -positive E. coli isolates were resistant to colistin. All three isolates showed a lower resistance to other classes of antibacterials, with all three being susceptible to carbapenems. The MLST results indicated that the three E. coli isolates were assigned to three different sequence types: ST457, ST101, and ST1413, respectively. The conjugation experiment showed that the mcr-1 gene was successfully transferred to the recipient ( E. coli EC600) from two isolates, one of which possessed IncI1 replicons and the other of which carried IncHI2 and IncN replicons. The patients with bloodstream infections caused by mcr-1 -positive isolates had severe underlying diseases and were cured after antibacterial treatment. Conclusion The prevalence of the mcr-1 gene in patients with E. coli bloodstream infection was 2.1% in Changsha, China. The mcr-1 -positive E. coli isolates had varied susceptibility profiles, although all three were susceptible to carbapenems. This therapeutic window is crucial given the risk of rapid deterioration in high-incidence areas worldwide.

show abstract

Characterization of Highly Prevalent Plasmids Coharboringmcr-1,oqxAB, andbla_CTX-Mand Plasmids HarboringoqxABandbla_CTX-MinEscherichia coliIsolates from Food-Producing Animals in China

Cited by 14 publications

References 37 publications

Occurrence and characterization of KPC‐2‐producing ST11 Klebsiella pneumoniae isolate and NDM‐5‐producing Escherichia coli isolate from the same horse of equestrian clubs in China

Occurrence and characterization of KPC‐2‐producing ST11 Klebsiella pneumoniae isolate and NDM‐5‐producing Escherichia coli isolate from the same horse of equestrian clubs in China

Genomic Characterization of mcr-1-carrying Salmonella enterica Serovar 4,[5],12:i:- ST 34 Clone Isolated From Pigs in China

<p>Epidemiology and molecular characterization of <em>mcr-1</em> in <em>Escherichia coli</em> recovered from patients with bloodstream infections in Changsha, central China</p>

Contact Info

Product

Resources

About