Characterization of high temperature-tolerant strains of Pyropia yezoensis

Shin, Yoon Ju; Min, Sung Ran; Kang, Da Yeon; Lim, Jong‐Min; Park, Eunjeong; Hwang, Mi Sook; Choi, Dong-Woog; Ahn, Joon-Woo; Park, Youn‐Il; Jeong, Won-Joong

doi:10.1007/s11816-018-0499-2

Cited by 16 publications

(3 citation statements)

References 12 publications

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“…As previously reported, temperature affects the growth and physiological metabolism of N. yezoensis; the optimal growth of the gametophyte thalli occurred at 10-15 • C [41], and was inhibited when cultured at 20 • C for 3 weeks [16]. To obtain a heat-resistant and high-yield strain of N. yezoensis, 23 • C and 24 • C were selected as the high temperature stress for screening [42,43].…”

Section: Introductionmentioning

confidence: 96%

“…Therefore, the breeding of strains with high yield and strong tolerance has become an important topic in N. yezoensis cultivation [13]. Currently, many studies have been conducted worldwide to develop high-temperature-resistant (HTR) strains of N. yezoensis by ray mutagenesis and intraspecific hybridization on gametophytic blades of N. yezoensis [14][15][16]. However, these studies remained at the individual level, and the underlying molecular mechanisms have not been understood till date.…”

Section: Introductionmentioning

confidence: 99%

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A Preliminary Study on the Mechanisms of Growth and Physiological Changes in Response to Different Temperatures in Neopyropia yezoensis (Rhodophyta)

Yin

Che³

et al. 2022

Water

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As an economically valuable red seaweed, Neopyropia yezoensis (Rhodophyta) is cultivated in intertidal areas, and its growth and development are greatly influenced by environmental factors such as temperature. Although much effort has been devoted to delineating the influence, the underlying cellular and molecular mechanisms remain elusive. In this study, the gametophyte blades and protoplasts were cultured at different temperatures (13 °C, 17 °C, 21 °C, 25 °C). Only blades cultured at 13 °C maintained a normal growth state (the relative growth rate of thalli was positive, and the content of phycobiliprotein and pigments changed little); the survival and division rates of protoplasts were high at 13 °C, but greatly decreased with the increase in temperature, suggesting that 13 °C is suitable for the growth of N. yezoensis. In our efforts to delineate the underlying mechanism, a partial coding sequence (CDS) of Cyclin B and the complete CDS of cyclin-dependent-kinase B (CDKB) in N. yezoensis were cloned. Since Cyclin B controls G2/M phase transition by activating CDK and regulates the progression of cell division, we then analyzed how Cyclin B expression in the gametophyte blades might change with temperatures by qPCR and Western blotting. The results showed that the expression of Cyclin B first increased and then decreased after transfer from 13 °C to higher temperatures, and the downregulation of Cyclin B was more obvious with the increase in temperature. The phosphorylation of extracellular signal-regulated kinase (ERK) decreased with the increase in temperature, suggesting inactivation of ERK at higher temperatures; inhibition of ERK by FR180204 significantly decreased the survival and division rates of protoplasts cultured at 13 °C. These results suggest that downregulation of Cyclin B and inactivation of ERK might be involved in negatively regulating the survival and division of protoplasts and the growth of gametophyte blades of N. yezoensis at high temperatures.

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Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

A Preliminary Study on the Mechanisms of Growth and Physiological Changes in Response to Different Temperatures in Neopyropia yezoensis (Rhodophyta)

Yin

Che³

et al. 2022

Water

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“…For instance, elevating temperature from 10°C to 20°C has decreased growth rates, phycoerythrin content and nitrogen removal rates in the species, Neopyropia leucosticta, Porphyra linearis and Porphyra umbilicalis (Kim et al, 2007). In the past decade, several heat-tolerant strains of N. yezoensis have been developed for the Neopyropia aquaculture (Ding et al, 2016;Shin et al, 2018). However, there are several findings that domestication resulted in the reduction in genetic diversity of the cultivated seaweed population (Voisin et al, 2005;Niwa and Aruga, 2006).…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis reveals the molecular mechanism of heat-tolerance in Neopyropia yezoensis induced by Sargassum horneri extract

Xing

Han²,

Park³

et al. 2023

Front. Mar. Sci.

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IntroductionHeat stress is one of the main threats for the seaweed aquaculture, leading to the decay of the blades and causing serious economic losses. A newly developed biostimulant, Sargassum horneri extract (SHE), can enhance the heat-tolerance in Neopyropia yezoensis, which can be applied in the seaweed aquaculture. However, the molecular mechanism underlying this acquired heat-tolerance is still unclear.MethodIn this study, we examined the transcriptional regulation of N. yezoensis under heat stress after the SHE pre-treatment by performing the RNA-Seq on illumina Novaseq platform and differentially expressed gene analysis.Result and discussionOur results show that, under heat stress, the SHE pre-treatment can alleviate the suppression of genes involved in normal cell activities, such as ribosome biogenesis, photosynthesis, respiration and cell cycle. On the other hand, SHE pre-treatment can also induce some key genes involved in the heat-tolerance. We also found that the combination of SHE pre-treatment and heat stress can induce some genes related to epigenetic modification, suggesting that SHE may facilitate the formation of heat stress memory in N. yezoensis. Our study provides information for understanding the molecular mechanism of heat-tolerance in N. yezoensis and for promoting the application of seaweed-derived biostimulants in the seaweed aquaculture.

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Non-targeted metabolomic analysis of Pyropia yezoensis metabolites using GC-MS and its regulation under high temperature

Zeb,

Yang,

Khan

et al. 2024

J. Ocean. Limnol.

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Characterization of high temperature-tolerant strains of Pyropia yezoensis

Cited by 16 publications

References 12 publications

A Preliminary Study on the Mechanisms of Growth and Physiological Changes in Response to Different Temperatures in Neopyropia yezoensis (Rhodophyta)

A Preliminary Study on the Mechanisms of Growth and Physiological Changes in Response to Different Temperatures in Neopyropia yezoensis (Rhodophyta)

Comparative transcriptome analysis reveals the molecular mechanism of heat-tolerance in Neopyropia yezoensis induced by Sargassum horneri extract

Non-targeted metabolomic analysis of Pyropia yezoensis metabolites using GC-MS and its regulation under high temperature

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