Characterization of Hg(II) binding with different length phytochelatins using liquid chromatography and amperometric detection

Dago, Àngela; González-García, Olga; Ariño, Cristina; Dı́az-Cruz, José Manuel; Esteban, Miquel

doi:10.1016/j.aca.2011.03.060

Cited by 14 publications

(13 citation statements)

References 30 publications

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“…The mobile phase consisted of 0.1% TFA in ultrapure filtered water pH = 2.00 and 0.1% TFA in acetonitrile. Separation was performed with gradient elution, as described in Dago et al 34 The electrochemical detector (ED), consisting of a flow cell (BAS, West Lafayette, IN, USA) and an Autolab PGSTAT-12 (Eco Chemie, Utrecht, The Netherlands), was coupled at the end of the column. The flow cell consisted of a glassy carbon working electrode (BAS) whose surface was polished daily, a stainless steel auxiliary electrode, and an Ag/AgCl (NaCl 3 mol L −1 ) reference electrode.…”

Section: Spe Procedures and Hplc-ed Analysis For The Determination Of...mentioning

confidence: 99%

Evaluation of Mercury Stress in Plants from the Almadén Mining District by Analysis of Phytochelatins and Their Hg Complexes

Dago

González

Ariño

et al. 2014

Environ. Sci. Technol.

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To evaluate plant response to Hg stress, glutathione, phytochelatins, and their Hg complexes were analyzed using HPLC with amperometric detection in samples of Asparagus acutifolius grown in the Almadén mining district (Ciudad Real, Spain), one of the most Hg-contaminated sites in the world. Soils of the Almadén mining district, and specifically from the Almadenejos zone, are highly contaminated, with some zones having values above 4,000 μg Hg g(-1) soil. Although soils have an extremely high concentration of mercury, generally less than 2% is available for plants, as is shown by various soil extractions simulating bioavailability. In plants, Hg concentration increases depending on the content of Hg in soils. In addition, Hg levels in roots are higher than in aerial parts, which is a strategy of plants for protecting their more sensitive aerial parts from the deleterious effects of metal stress. The total content of phytochelatins (PCs) and their complexes are directly related with the amount of mercury in soils. These findings highlight the important role of thiol compounds and their metal complexes in capturing and fixing Hg from soils, giving plants the capacity to deal with the heavy metal toxicity of polluted soils.

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Section: Spe Procedures and Hplc-ed Analysis For The Determination Of...mentioning

confidence: 99%

Evaluation of Mercury Stress in Plants from the Almadén Mining District by Analysis of Phytochelatins and Their Hg Complexes

Dago

González

Ariño

et al. 2014

Environ. Sci. Technol.

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“…These results suggested that the developed method for the determination of the six analytes was accurate and sensitive. Compared with other reported methods [27][28][29][30][31], similar response ranges but higher detection limits were achieved with the HPLC-ELSD method compared with the HPLC-fluorescence (Flu) method. However, the HPLC-Flu method has a complicated derivatization process, whereas low detection limits can be obtained with the HPLC-ELSD method relative to the HPLC-ED or ion-pair chromatography (IPC)-MS methods (table 2).…”

Section: Calibration Curves Linear Ranges and Detection Limitsmentioning

confidence: 68%

Development of the HPLC–ELSD method for the determination of phytochelatins and glutathione inPerilla frutescensunder cadmium stress conditions

et al. 2018

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A rapid, accurate and simple method was developed for the simultaneous determination of glutathione (GSH) and phytochelatins (PCs) by high-performance liquid chromatography (HPLC) with an evaporative light-scattering detector. GSH, phytochelatin 2 (PC2), PC3, PC4, PC5 and PC6 can be separated with baseline separation within 9 min using a Venusil AA column (250 mm × 4.6 mm i.d., 5 µm particle sizes). Acetonitrile and water containing 0.1% trifluoroacetic acid (0.1%) were employed as the mobile phase for the gradient elution. The drift tube temperature and flow rate of the carrier gas (N2) were 50°C and 1.5 l min−1, respectively. Under optimum conditions, good linear regression equations of six analytes were obtained with the detection limits ranging from 0.2 to 0.5 µg ml−1. The proposed method has been applied successfully for the quantification of GSH and PCs in Perilla frutescens (a cadmium hyperaccumulator) under cadmium stress. The recoveries were between 82.9% and 115.3%.

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“…1% TFA in acetonitrile was used. Separation was performed with gradient elution, as described inDago et al, 2011. For the analysis of PC isoforms…”

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confidence: 99%

Phytochelatin synthesis in response to Hg uptake in aquatic plants near a chlor-alkali factory

et al. 2017

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Characterization of Hg(II) binding with different length phytochelatins using liquid chromatography and amperometric detection

Cited by 14 publications

References 30 publications

Evaluation of Mercury Stress in Plants from the Almadén Mining District by Analysis of Phytochelatins and Their Hg Complexes

Evaluation of Mercury Stress in Plants from the Almadén Mining District by Analysis of Phytochelatins and Their Hg Complexes

Development of the HPLC–ELSD method for the determination of phytochelatins and glutathione inPerilla frutescensunder cadmium stress conditions

Phytochelatin synthesis in response to Hg uptake in aquatic plants near a chlor-alkali factory

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