Characterization of hepatic progenitors from human fetal liver during second trimester

Rao, Mekala Subba; Khan, Aleem Ahmed; Parveen, Nyamath; Habeeb, Mohammed Aejaz; Habibullah, C. M.; Pande, Gopal

doi:10.3748/wjg.14.5730

Cited by 39 publications

(26 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was noteworthy that newborn mLEPCs expressed E-cad, CD49f, and nestin, which have been documented as the specific markers of HPCs in many literatures. CD49f has been detected in fetal as well as adult mouse liver, in states of liver disease, and in liver hepatic stem cells (Suzuki et al 2002;Hoppo et al 2004;Rao et al 2008). Nestin, generally recognized as a specific marker for neuroectodermal progenitors, has been detected in foetal hepatic stem cells (Sun and An 2004;Herrera et al 2006) or in proliferating adult hepatocytes (Koenig et al 2006a).…”

Section: Discussionmentioning

confidence: 96%

Culture of newborn monkey liver epithelial progenitor cells in chemical defined serum-free medium

Jin

Guo

et al. 2010

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

Studies with hepatic progenitor cells from non-human primates would allow better understanding of their human counterparts. In this study, rhesus monkey liver epithelial progenitor cells (mLEPCs) were derived from a small piece of newborn livers in chemical defined serum-free medium. Digested hepatic cells were treated in Ca(2+)-containing medium to form cell aggregates. Two types of cell aggregates were generated: elongated spindle cells and polygonal epithelial cells. Elongated spindle cells were expressed as vimentin and brachyury, and they were disappeared within 5 d in our cultures. The remaining type consisted of small polygonal epithelial cells that expressed cytokeratin 7 (CK7), CK8, CK18, nestin, CD49f, and E-cad, the markers of hepatic stem cells, but were negative for alpha-fetoprotein, albumin, and CK19. They can proliferate and be passaged, if on laminin or rat tail collagen gel, to initiate colonies. When cultured with dexamethasone and oncostatin M, the expression of mature hepatocyte markers, such as alpha-1-antitrypsin, intracytoplasmic glycogen storage, indocyanine green uptake, and lipid droplet generation, were induced in differentiated cells. If transferred onto mouse embryonic fibroblasts feeders, they gave rise to CK19-positive cholangiocytes with formation of doughnut-like structure. Thus, mLEPCs with bipotency were derived from newborn monkey liver and may serve as a preclinical model for assessment of cell therapy in humans.

show abstract

Section: Discussionmentioning

confidence: 96%

Culture of newborn monkey liver epithelial progenitor cells in chemical defined serum-free medium

Jin

Guo

et al. 2010

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

show abstract

“…Just in 2007, FLSPCs were isolated by MACS with epithelial cell adhesion molecule (EpCAM), which were also CD133 positive [8]. Later in 2008, EpCAM + FLSPCs were demonstrated positive for several markers associated with hepatic progenitor cells including CD49 [23]. Actually, CD49f has been used as an independent marker to isolate FLSPCs [9,24].…”

Section: Discussionmentioning

confidence: 99%

Convenient and Efficient Enrichment of the CD133+ Liver Cells from Rat Fetal Liver Cells as a Source of Liver Stem/Progenitor Cells

Liu

Ren

Dou

2010

Stem Cell Rev and Rep

View full text Add to dashboard Cite

Although the stem cells are commonly isolated by FACS or MACS, they are very expensive and these is no specific marker for liver stem/progentior cells (LSPCs). This paper applied a convenient and efficient method to enrich LSPCs. The fetal liver cells (FLCs) were firstly enriched by Percoll discontinuous gradient centrifugation (PDGC) from the rat fetal liver. Then the FLCs in culture were purified to be homogeneous in size by differential trypsinization and differential adherence (DTDA). Flow cytometric analysis revealed more than half of the purified FLCs expressed alternative markers of LSPCs (CD117, c-Met, Sca-1, CD90, CD49f and CD133). In other words, the purified FLCs were heterogeneous. Therefore, they were sequentially layered into six fractions by Percoll continuous gradient centrifugation (PCGC). Both CD133 and CD49f expressed decreasingly from fraction 1 to 6. In fraction 1 and 2, about 85% FLCs expressed CD133, which were revealed to be LSPCs by high expressions of AFP and CK-19, low expressions of G-6-P and ALB. To conclude, the purity of CD133(+) LSPCs enriched by combination of PDGC, DTDA and PCGC is close to that obtained by MACS. This study will greatly contribute to two important biological aspects: liver stem cells isolation and liver cell therapy.

show abstract

“…The self-renewal capacity of HSCs was demonstrated by phenotypic stability after expansion for more than 150 population doublings in a serum-free, defined medium and with a doubling time of 36 h (36). Recently, several studies have indicated the ability of rapid proliferation and regeneration of liver cells derived from human fetal liver compared with the adult liver (37)(38)(39)(40). Earlier work has shown that treatment for acute liver failure by human fetal hepatocytes is feasible (41).…”

Section: Discussionmentioning

confidence: 99%

Stem cell transplantation for the treatment of liver diseases: A systematic review and meta-analysis

Liu

Li²,

Li³

et al. 2016

Turk J Gastroenterol

View full text Add to dashboard Cite

Characterization of hepatic progenitors from human fetal liver during second trimester

Abstract: Our study suggests that these EpCAM +ve cells can be used as hepatic progenitors for cell transplantation with a minimum risk of alloreactivity and these cells may serve as a potential source for enrichment of hepatic progenitor.

Cited by 39 publications

References 21 publications

Culture of newborn monkey liver epithelial progenitor cells in chemical defined serum-free medium

Culture of newborn monkey liver epithelial progenitor cells in chemical defined serum-free medium

Convenient and Efficient Enrichment of the CD133+ Liver Cells from Rat Fetal Liver Cells as a Source of Liver Stem/Progenitor Cells

Stem cell transplantation for the treatment of liver diseases: A systematic review and meta-analysis

Contact Info

Product

Resources

About