Characterization of hemocytes proliferation in larval silkworm, Bombyx mori

A novel granulocyte-specific α integrin is essential for cellular immunity in the silkworm Bombyx mori

“…Dazao (p50) from the Gene Resource Library of Domesticated Silkworm of Southwest University, China, were reared with mulberry leaves as described previously (Tan et al, 2013). Haemocytes were collected from silkworm larvae by cutting a leg.…”

“…Collected haemocytes were cultured on coverslips for 30 min at room temperature as described previously (Tan et al, 2013). The cells were fixed in 4% (w/v) paraformaldehyde (PFA) for 15 min.…”

“…The silkworm Bombyx mori has five types of haemocytes: prohaemocytes, plasmatocytes, granulocytes, spherulocytes, and oenocytoids (Tan et al, 2013). Our previous work identified the potential haematopoietic stem cell or progenitor, which may contribute to larval haematopoiesis in the silkworm (Tan et al, 2013).…”

“…Our previous work identified the potential haematopoietic stem cell or progenitor, which may contribute to larval haematopoiesis in the silkworm (Tan et al, 2013). Silkworm larval haemocytes in haematopoietic organs (HPOs) were divided into two major lineages: a granulocyte lineage and a plasmatocyte-oenocytoid lineage (Nakahara et al, 2010a).…”

The immune response of silkworm, Bombyx mori

“…mori larvae contain several thousand hemocytes, which can be divided into the following three cell types on the basis of their structural and functional features: plasmatocytes, crystal cells and lamellocytes (Lemaitre and Hoffmann, 2007;Tan et al, 2013;Wago, 1982;Williams, 2007). Plasmatocytes are professional phagocytes that function in the phagocytic removal of dead cells and microbial pathogens, which is most similar to the mammalian macrophage lineage and make up 95% of circulating hemocytes.…”