Characterization of heavy metal and antibiotic-resistant bacteria isolated from polluted localities in Egypt

Moghannem, Saad A.; Refaat, B. M.; El-Sherbiny, Gamal M.; Elsayed, Mohamed A.; Elsehemy, Islam A.; Kalaba, Mohamed H.

doi:10.4103/1687-4315.172856

Cited by 19 publications

(16 citation statements)

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“…This is consistent with the results of the previous study where the growth of selected metal-tolerance strains was only slightly affected by different temperatures and pH values within a biological range (Moghannem et al 2015).…”

Section: Determination Of the Effect Of Iron (Feso 4 ) On The Growth supporting

confidence: 82%

“…Similar results have been reported by others (Raja et al 2009;Moghannem et al 2015;Khanet al 2016). This behavior could be explained by the fact that exposure to metal stress likely directs the energy budget of the exposed bacteria away from growth towards the maintenance of other functions that enable the bacteria to resist metal toxicity (Alboghobeish et al 2014).…”

Section: Determination Of the Effect Of Iron (Feso 4 ) On The Growth supporting

confidence: 82%

“…Encouraged by these preliminary findings, the multi-metal tolerance of isolates against Pb, Cu, Cr, Ag, and Hg at different concentrations was evaluated using MIC technique which is a powerful and easy technique for determination of heavy metal resistance (Neethu et al 2015) (Table1). Beside high iron tolerance capacity with MIC values up to 550-600 mg/L and MTC larger than 2000 mg/L, isolates were found to exhibit different multiple heavy metal tolerance characteristics to Pb, Cu, Cr, Ag, and Hg, this phenomenon is common among isolated heavy metal tolerance microbes (Alboghobeishet al 2014;Moghannem et al 2015). The high level of iron oxides that occur naturally in rock varnish (Parchert et al 2012) appears to have given the indigenous bacterial strains a remarkable level of tolerance to iron.…”

Section: Determination Of the Effect Of Iron (Feso 4 ) On The Growth mentioning

confidence: 99%

“…Following incubation at 30°C for 48 hours, (10 μL) from each well was spotted on nutrient agar plates and incubated at 37°C for 24-72 hours to test any appearance of growth. The maximum tolerable concentration (MTC) of iron (II) is defined as the highest concentration of iron (II) which allows growth after 48 h (Moghannem et al 2015).…”

Section: Determination Of Maximum Tolerable Concentrations (Mtcs) Of mentioning

confidence: 99%

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Iron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan

et al. 2017

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Abstract. Alnaimat S, Shattal SA, Althunibat O, Alsbou E, Amasha R. 2017. Iron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan. Biodiversitas 18: 1250-1257. This study deals with enumeration, identification and molecular characterization of bacterial species tolerant of iron (II) and other heavy metals that isolated from rock varnish collected from the arid region of Al-Jafer Basin, Jordan. Based on conventional culture-dependent methods, six isolates exhibited a high degree of tolerance to iron (II) and other heavy metals with a minimum inhibitory concentration (550-600 mg/L) to iron, (500-550 mg/L) to lead, (350 mg/L) to copper, (400-450 mg/L) to chromium, (150-200 mg/L) to silver, and (150-200 mg/L) to mercury, in solid media. The results showed that all isolates could tolerate 2000 mg/L Fe 2+ expressed as maximum tolerance concentration (MTC). The multiple metal resistances of these isolates were also associated with multiple antibiotic resistances. These iron tolerant isolates were shown to be capable of growth at temperatures ranging from 30 to 40°C and pH values ranging from 5 to 9 with some differences between the six isolates. On the basis of morphology and of 16S rDNA gene sequencing and phylogeny analysis, the iron-tolerant isolates were identified as Bacillus zhangzhouensis (DVCr1), Staphylococcus warneri (DVCr6), Bacillus cereus (DVCr7), Staphylococcus pasteuri (DVCu2), Brevibacterium frigoritolerans (DVPb) and Bacillus altitudinis (DVCr10). The 16S rDNA sequences from five of the six strains were submitted to GenBank and are available under the accession numbers KX781139, KX781140, KX781141, KX781145 and KX781148. The results highlight the high potential of a non-polluted habitat like rock varnish to provide new microbial communities that could be used for enhanced bioremediation of heavy metals from a contaminated site.

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Section: Determination Of the Effect Of Iron (Feso 4 ) On The Growth supporting

confidence: 82%

Section: Determination Of the Effect Of Iron (Feso 4 ) On The Growth supporting

confidence: 82%

Section: Determination Of the Effect Of Iron (Feso 4 ) On The Growth mentioning

confidence: 99%

Section: Determination Of Maximum Tolerable Concentrations (Mtcs) Of mentioning

confidence: 99%

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Iron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan

et al. 2017

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“…The microbial growth on solid media did not give a correct picture of metal tolerance where the complexation, diffusion, and availability of metals differ from those in the broth, agar had protecting effects and chelate metal ions. In consequence, the heavy metals became slightly available for the growth, giving a miss-indication to a higher tolerance response ( Moghannem et al., 2015 ), therefore, tolerance assay compared in both solid and liquid media.…”

Section: Resultsmentioning

confidence: 99%

Bioremediation and tolerance of zinc ions using Fusarium solani

Sayed

El-Sayed

2020

Heliyon

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Evaluating the mechanism of tolerance and biotransformation Zn(II) ions by Fusarium solani based on the different physiological was the objective of this work. The physical properties of synthesized ZnONPs was determined by UV-spectroscopy, transmission electron microscope, and X-ray powder diffraction. The structural and anatomical changes of F. solani in response to Zn(II) was examined by TEM and SEM. From the HPLC profile, oxalic acid by F. solani was strongly increased by about 10.5 folds in response to 200 mg/l Zn(II) comparing to control cultures. The highest biosorption potential were reported at pH 4.0 (alkali-treated biomass) and 5.0 (native biomass), at 600 mg/l Zn(II) concentration, incubation temperature 30 °C, and contact time 40 min (alkali-treated biomass) and 6 h (native biomass). From the FT-IR spectroscopy, the main functional groups implemented on this remediation were C–S stretching, C=O C=N, C–H bending, C–N stretching and N–H bending. From the EDX spectra, fungal cellular sulfur and phosphorus compounds were the mainly compartments involved on ZN(II) binding.

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