Characterization of heat shock protein 70 in the red claw crayfish (Cherax quadricarinatus): Evidence for its role in regulating spermatogenesis

Molecular cloning, expression pattern, and chemical analysis of heat shock protein 70 (HSP70) in the mudskipper Boleophthalmus pectinirostris: Evidence for its role in regulating spermatogenesis

“…Previous studies have reported HSP70 expression in all major tissues, such as the heart, blood, brain, liver, muscle, gill, kidney, and spleen (Fang et al 2012;Rajeshkumar et al 2013;Yue et al 2011). Here, the expression of HSP70 in various tissues from mudskipper, including testis, was studied for the first time.…”

“…In the testes, spermatids undergo dramatic morphological changes and differentiate into mature sperm during spermiogenesis, a process that is regulated by a series of complex signaling pathways (He et al 2009) and the variable expression of proteins such as Heat Shock Proteins (HSPs) (Allen et al 1996;Fang et al 2012). HSPs, also called Heat Stress Proteins, are a family of conserved cellular proteins that are found in all organisms from lower prokaryotes to higher mammals (Gupta and Golding, 1993;Lindquist and Craig, 1988).…”

“…HSPs play an important biological role under both normal and stress conditions. Under normal conditions, HSPs act as molecular chaperones, and are involved in protein metabolism, cell cycle regulation, apoptosis, and spermatogenesis (Fang et al 2012;Hightower, 1991;Hendrick and Hartl, 1993;Welch, 1993). When organisms are subjected to stress by stimuli, such as changes in temperature, dissolved oxygen levels, osmotic pressure, heavy metals, and the presence microbial infections, HSP expression is significantly increased, enabling organisms to resist these insults and to maintain homeostasis Liu et al 2015;Ming et al 2010;Su et al 2010;Vazzana et al 2015;Wang et al 2015).…”

Molecular cloning and characterization of the lipopolysaccharide and β-1,3-glucan binding protein from red claw crayfish, Cherax quadricarinatus

“…It has become an important species in Jiangsu, Zhejiang, Fujian and Guangdong provinces aquaculture since it was introduced to China. It is an important species in freshwater aquaculture (Fang et al, 2011(Fang et al, , 2012. However, the molecular biology study of C. quadricarinatus is insufficient.…”

“…Only a few genes have been reported, such as heat shock protein 70, SOD, etc. (Fang et al, 2011(Fang et al, , 2012Gu et al, 2014;Liu et al, 2011). Therefore, understanding the immune ability of C. quadricarinatus and its defense mechanisms has become a primary concern.…”