Characterization of heat shock protein 90, 70 and their transcriptional expression patterns on high temperature in adult of Grapholita molesta (Busck)

Abstract

Grapholita molesta (Busck) is a worldwide insect pest damaging stone and pome fruits. High temperature can significantly affect insect survival, development and fecundity. Heat shock protein (Hsp) genes were speculated to possess a pivotal function in response to high temperature stress. In this study, two full-length Hsp genes, Gmhsp90 and Gmhsp70, were cloned from G. molesta using rapid amplification of complementary DNA ends (RACE). The open reading frames of Gmhsp90 and Gmhsp70 obtained were 2 148 bp and 1 998 bp in length, respectively. Their deduced amino acids showed high homology to Hsp genes of other species. Subsequently, the transcriptional expression of Gmhsp90 and Gmhsp70 in G. molesta adults exposed at various temperatures (26, 29, 32, 35, 38, 41 and 44°C) for 1 h and at 41°C for various time duration (0, 15, 30, 45, 60, 75, 90 and 105 min) were investigated via real time quantitative polymerase chain reaction (qPCR). The relative expression levels of Gmhsp90 and Gmhsp70 in G. molesta adults were both up-regulated with the rise of temperature and time duration. In addition, the Gmhsp70 usually showed a higher transcription accumulation than Gmhsp90. Interestingly, Gmhsp90 and Gmhsp70 in female adults could be induced much earlier than that in males, and the effective induction temperature in females was also lower than that in males. The distinct expression profiles of Gmhsp90 and Gmhsp70 indicated that Gmhsp90 and Gmhsp70 may play important roles in G. molesta adults responding to a thermal threat, and there is difference on induction between sexes.

Response of heat shock protein genes of the oriental fruit moth under diapause and thermal stress reveals multiple patterns dependent on the nature of stress exposure

“…One of GmHsp70 members previously uploaded in GenBank JX678981 (Chen et al 2014) showed 84 % similarity of full-length amino acid sequence with the gene we studied. Both our GmHsp70 and the one studied by Chen et al (2014) have a conserved C-terminal motif BEEVD^with a potential binding function, suggesting a role in protein folding/refolding (Michels et al 1999;Vabulas et al 2010). Information on Hsp genes from model species might help to identify functional roles of GmHsps, such as the stress-inducible pattern in other species.…”

“…The 12-h enclosed moths (equal numbers of males and females) were therefore kept at −5°C (cold) or 42°C (heat) for 1 and 2 h before recovering at 25°C for 2 h. The 42°C temperature is close to 41°C which Chen et al (2014) previously found to elicit higher expression of Hsp70 and Hsp90 in G. molesta compared to lower temperatures or 44°C. No data have previously been collected on lower limits of adults.…”

“…We also used other OFM Hsp genes for extreme temperature expression, including GmHsp11. 1, 18.9, 19.8, 19.9, 21.4, 22.5, 31.8 (Zhang et al 2015), GmHsp70 (but labeled as GmHsp70-2 in figure), and GmHsp90 (Chen et al 2014).…”

Transcriptome Analysis and Identification of Major Detoxification Gene Families and Insecticide Targets in Grapholita Molesta (Busck) (Lepidoptera: Tortricidae)

“…Despite the economic importance of G. molesta , at the time of this research, only a limited number of studies had been conducted at the molecular level, including the sequencing of the mitochondrial genome (Gong et al 2012), characterization of two general odorant binding protein genes (Zhang et al 2012), population genetic structure (Kirk et al 2013), transcriptome analysis of sex pheromone glands (Jung and Kim 2014), and characterization of two heat shock proteins (Chen et al 2014). Although these studies generated rich genomic and EST sequence data for G. molesta , it lacked sequence information related to detoxification or other insecticide related genes.…”

Histone Acetylation Modifications Affect Tissue-Dependent Expression of Poplar Homologs of C4 Photosynthetic Enzyme Genes

“…For the analysis of the expression of poplar homologs of C 4 photosynthetic enzyme genes, all samples were normalized to the reference gene PsnACTIN2 . The final relative expression was calculated using the formula F = 2 -ΔΔ Ct (Chen et al, 2014). …”