Characterization of Heat-labile Uracil-DNA Glycosylase from
Oncorhynchus mykiss and its Application for Carry-over Contamination
Control in RT-qPCR
Qingyuan Huang,
Yaqi Zhang,
Wenhao Hu
et al.
Abstract:Background:
Heat-labile uracil-DNA glycosylase (HL-UDG) is commonly employed
to eliminate carry-over contamination in DNA amplifications. However, the prevailing HL-UDG
is markedly inactivated at 50°C, rendering it unsuitable for specific one-step RT-qPCR protocols
utilizing reverse transcriptase at an optimal temperature of 42°C.
Objective:
This study aimed to explore novel HL-UDG with lower inactivation temperature and
for recombinant expression.
Methods:
The gene encoding an HL-UDG was cloned from the c… Show more
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