Characterization of halotolerant Thermus isolates from shallow marine hot springs on S. Miguel, Azores

Manaia, Célia M.; Costa, Milton S. da

doi:10.1099/00221287-137-11-2643

Cited by 55 publications

(32 citation statements)

References 15 publications

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“…Many strains of Thermus and Meiothermus have been isolated from terrestrial geothermal environments, although some strains, such as T. thermophilus Gy1211, have been obtained from shallow and deep-sea hydrothermal vent environments (Hudson et al, 1986;Kristjánsson et al, 1986;Hjörleifsdó ttir et al, 1989;Manaia & da Costa, 1991;Marteinsson et al, 1999). A distinctive physiological feature commonly displayed by all Thermus strains, including marine isolates, is that they do not show an absolute requirement for NaCl for growth.…”

Section: Ecological Nichementioning

confidence: 99%

Marinithermus hydrothermalis gen. nov., sp. nov., a strictly aerobic, thermophilic bacterium from a deep-sea hydrothermal vent chimney

Sako

Nakagawa

Takai³

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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A novel thermophilic marine bacterium, designated strain T1 T , was isolated from a deep-sea hydrothermal vent chimney sample collected from the Suiyo Seamount in the Izu-Bonin Arc, Japan, at a depth of 1385 m. Cells of strain T1 T were rod-shaped, occurring in pairs or filamentous, and stained Gram-negative. Growth was observed between 50?0 and 72?5˚C (optimum 67?5˚C; 30 min doubling time) and at pH 6?25-7?75 (optimum pH 7?00). The isolate absolutely required NaCl, at a concentration of 0?5-4?5 % (optimum 3?0 %). It was a strictly aerobic heterotroph capable of growing solely on complex organic substrates such as yeast extract, tryptone and Casamino acids, utilizing glutamate, proline, serine, cellobiose, trehalose, sucrose, acetate and pyruvate as complementary substrates. The G+C content of the genomic DNA was 68?6 mol%. The 16S rRNA gene sequence of the isolate was most similar to those from members of the genus Thermus, but the isolate was distantly related to them at the genus level (,90 %). In addition, phylogenetic analysis indicated that the isolate was on a novel lineage, deeply branched prior to divergence of the genus Thermus. On the basis of phylogenetic analysis and physiological traits of the isolate, it should be described as a member of a novel genus distinct from the previously described genus Thermus. The name Marinithermus gen. nov. is proposed, with Marinithermus hydrothermalis gen. nov., sp. nov. as the type species. The type strain of M. hydrothermalis gen. nov., sp. nov. is strain T1 T (5JCM 11576 T 5DSM 14884 T ). INTRODUCTIONA number of aerobic thermophiles have been isolated from a variety of geothermal environments such as hot springs, solfataric fields and hydrothermal vents throughout the world (Brock & Freeze, 1969;Brock et al., 1972;Grogan et al., 1990;Huber et al., 1992; Sako et al., 1996a Sako et al., , b, 2001). Members of the genus Thermus are probably the most frequently isolated extremely thermophilic aerobes from terrestrial hot-water environments. Although some strains grow mixotrophically (Skirnisdottir et al., 2001), almost all strains of the genus Thermus are obligate heterotrophs that live in various terrestrial hot environments at temperatures higher than about 55˚C and neutral to alkaline pH (Brock & Boylen, 1973;Pask-Hughes & Williams, 1975; Kristjánsson & Alfredsson, 1983;Williams & da Costa, 1992). Under anaerobic conditions, some strains of the genus Thermus are able to grow with NO { 3 , Fe(III) and S 0 as terminal electron acceptors instead of O 2 (Williams & da Costa, 1992;Sharp et al., 1995;Kieft et al., 1999).In deep-sea hydrothermal vent environments, however, most thermophiles had been thought to be strict anaerobes until the recent discovery of facultatively aerobic thermophiles from deep-sea hydrothermal vent chimneys (Blöchl et al., 1997; Reysenbach et al., 2000a). In addition, many strictly aerobic and thermophilic bacterial strains, including Thermus thermophilus Gy1211, have been isolated from deep-sea hydrothermal vent environments in the MidAtla...

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Section: Ecological Nichementioning

confidence: 99%

Marinithermus hydrothermalis gen. nov., sp. nov., a strictly aerobic, thermophilic bacterium from a deep-sea hydrothermal vent chimney

Sako

Nakagawa

Takai³

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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“…Hydrolysis of casein, starch (Manaia & da Costa, 1991) and gelatin (Smibert & Krieg, 1981) and degradation of arbutin, aesculin (Manaia & da Costa, 1991), elastin, hide-powder azure and fibrin (Hudson et al, 1986) were tested by using Thermus medium. Strain AZM34c11 T was positive for hydrolysis of starch and gelatin and for the degradation of hide-powder azure, but was negative for the other reactions.…”

mentioning

confidence: 99%

Meiothermus hypogaeus sp. nov., a moderately thermophilic bacterium isolated from a hot spring

Mori

Iino

Ishibashi

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

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A novel moderately thermophilic, red-pigmented bacterium, designated strain AZM34c11 T , was isolated from the bottom of a 1000 m-deep drilled well located in a Japanese hot spring. Cells were Gram-negative and grew optimally at 50 6C, at pH 7.6 and with 0-0.3 % (w/v) NaCl. Analysis of the 16S rRNA gene sequence revealed that the isolate belonged to the genus Meiothermus. Levels of 16S rRNA gene sequence similarity between strain AZM34c11 T and the type strains of recognized Meiothermus species were 88.2-94.8 %. Strain AZM34c11 T was distinguished from recognized Meiothermus species by its cellular fatty acid profile: iso-C 16 : 0 was one of the predominant components and hydroxy fatty acids were present only in trace amounts. The strain could also be differentiated based on its growth substrate preferences and characteristic enzyme reactions. On the basis of these results, strain AZM34c11 T is considered to represent a novel species of the genus Meiothermus, for which the name Meiothermus hypogaeus sp. nov. is proposed. The type strain is AZM34c11 T (5NBRC 106114 T 5DSM 23238 T ).

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“…Cultures were incubated at 65 u C for 7 days as recommended by Santos et al (1989). Other physiological and biochemical characteristics were examined as described by Hudson et al (1986), Manaia & da Costa (1991) and Chung et al (2000) in Thermus medium or on Thermus agar at 70 u C for 4 days.…”

mentioning

confidence: 99%

Thermus arciformis sp. nov., a thermophilic species from a geothermal area

Zhang

Ying

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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Two aerobic, Gram-negative, non-motile, non-sporulating, yellow-pigmented bacteria, strains TH92 T and TH91, were isolated from a hot spring located in Laibin, Guangxi, in the south-eastern geothermal area of China. The isolates grew at 40-77 6C (optimally at 70 6C) and at pH 6.0-9.5 (optimally at pH 7.5-8.0). Phylogenetic analysis of 16S rRNA gene sequences and levels of DNA-DNA relatedness together indicated that the new isolates represented a novel species of the genus Thermus with closest affinity to Thermus aquaticus, Thermus igniterrae and Thermus thermophilus. Compared with their closest relatives, strains TH92 T and TH91 were able to assimilate a wider range of carbohydrates, amino acids and organic acids as sole carbon sources for growth, such as lactose and melibiose. The new isolates had lower combined levels of C 16 : 0 and iso-C 16 : 0 compared with their closest relatives. On the basis of polyphasic taxonomic characterization, strains TH92 T and TH91 are considered to represent a single novel species of the genus Thermus, for which the name Thermus arciformis sp. nov. is proposed. The type strain is TH92 T (5CGMCC 1.6992 T 5JCM 15153 T ).

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Characterization of halotolerant Thermus isolates from shallow marine hot springs on S. Miguel, Azores

Cited by 55 publications

References 15 publications

Marinithermus hydrothermalis gen. nov., sp. nov., a strictly aerobic, thermophilic bacterium from a deep-sea hydrothermal vent chimney

Marinithermus hydrothermalis gen. nov., sp. nov., a strictly aerobic, thermophilic bacterium from a deep-sea hydrothermal vent chimney

Meiothermus hypogaeus sp. nov., a moderately thermophilic bacterium isolated from a hot spring

Thermus arciformis sp. nov., a thermophilic species from a geothermal area

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