Characterization of halophiles isolated from solar salterns in Baja California, Mexico

Sabet, Shereen; Diallo, Lamine; Hays, Lauren; Jung, Woo‐Sik; Dillon, Jesse G.

doi:10.1007/s00792-009-0247-1

Cited by 49 publications

(49 citation statements)

References 67 publications

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“…All fell within three genera: Haloarcula, Halorubrum, and Halobacterium. Representatives of these genera have been widely isolated from hypersaline environments Burns et al 2004;Maturrano et al 2006;Sabet et al 2009;Caton et al 2009;MakhdoumiKakhki et al 2012;Dillon et al 2013). The most abundant haloarchaeon was Haloarcula isolated from the Fadlum, Tuzlagözü, and Bingöl salterns.…”

Section: Isolation Of Bacteria and Archaeamentioning

confidence: 98%

“…There are many reports related to the microbiota of coastal salterns fed by sea water Burns et al 2004;Baati et al 2008;Sabet et al 2009), but few studies of inland salterns fed by hypersaline spring water (Maturrano et al 2006). These four salterns have no connection with the sea, but their salt compositions resemble thalassohaline environments (Oren 2002), derived from evaporated seawater, and are rich in sodium ions.…”

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confidence: 97%

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Comparative analysis of prokaryotic diversity in solar salterns in eastern Anatolia (Turkey)

Çınar

Mutlu

2016

Extremophiles

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The prokaryotic communities of four salterns (Bingöl, Fadlum, Kemah, and Tuzlagözü) in Turkey were examined and compared using the cultivation and cultivation-independent methods [fluorescence in situ hybridization (FISH) and 454 pyrosequencing]. FISH analysis with universal probes revealed that feeding waters carried 1.6 × 10(2)-1.7 × 10(3) cells mL(-1), while crystallization ponds carried 3.8 × 10(6)-2.0 × 10(7) cells mL(-1) that were mostly haloarchaea, including square cells (except for Kemah). High-throughput 16S rRNA-based gene sequencing showed that the most frequent archaeal OTUs in Bingöl, Fadlum, Tuzlagözü, and Kemah samples were affiliated with Haloquadratum (76.8 %), Haloarcula (27.8 %), Halorubrum (49.6 %), and Halonotius (59.8 %), respectively. Bacteroidetes was the dominant bacterial phylum in Bingöl and Fadlum, representing 71.5 and 79.5 % of the bacterial OTUs (respectively), while the most abundant bacterial phylum found in the Kemah saltern was Proteobacteria (79.6 %). The majority of the bacterial OTUs recovered from Tuzlagözü belonged to the Cyanobacteria (35.7 %), Bacteroidetes (35.0 %), and Proteobacteria (25.5 %) phyla. Cultivation studies revealed that the archaeal isolates were closely related to the genera Halobacterium, Haloarcula, and Halorubrum. Bacterial isolates were confined to two phyla, Proteobacteria (Alphaproteobacteria and Gammaproteobacteria classes) and Bacteroidetes. Comparative analysis showed that members of the Euryarchaeota, Bacteroidetes, Proteobacteria, and Cyanobacteria phyla were major inhabitants of the solar salterns.

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Section: Isolation Of Bacteria and Archaeamentioning

confidence: 98%

mentioning

confidence: 97%

Comparative analysis of prokaryotic diversity in solar salterns in eastern Anatolia (Turkey)

Çınar

Mutlu

2016

Extremophiles

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“…On the other hand, solar salterns found around the world, are extremely hyper saline habitats containing microbial systems (Sabet et al 2009 ). Mexico has some solar salterns for example, Las Coloradas in Yucatan State, and Guerrero Negro in Baja California Sur State.…”

Section: Saline Environmentsmentioning

confidence: 99%

Isolation and Screening of Halophilic Bacteria for Production of Hydrolytic Enzymes

Delgado-García

Nicolaus²,

Poli³

et al. 2015

Sustainable Development and Biodiversity

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There is a great interest on study of extreme microorganisms because of their special characteristics in biotechnology. Particularly, halophilic microorganisms which live in saline environments throughout of their different adaptation mechanisms produce metabolites with great potential. Some of its biomolecules has been studied and applied in industrial processes, such as exopolyssaccharides, carotenoid pigments, bacteriorhodopsin etc. beside certain enzymes especially hydrolases (pectinases, amylases, proteases, lipases, etc.) are important. Recent researches on halophilic microorganisms and their biomolecules have increased around the world. Saline environments such as saline lakes or saline soils are excellent sources for isolation of halophilic microorganisms. However, few saline environments have been studied in depth in order to evaluate the special characteristics of halophilic biomolecules. In this review, the importance of halophilic microorganisms for biotechnological industries, methods for their isolation; techniques for physiological, taxonomical and molecular characterization have been highlighted so as to establish them as important source for enzyme production.

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“…Pure isolates of halophilic archaea currently include 496 species distributed among 27 genera, with genome sequence information available for more than a dozen species (Oren et al, 2009). Numerous cultivation-independent biodiversity surveys have been performed in hypersaline environments using PCR amplification of archaeal and bacterial 16S ribosomal RNA (rRNA) genes, as well as direct metagenomic sequencing of community DNA (Grant et al, 1999;Benlloch et al, 2001;Ochsenreiter et al, 2002;Burns et al, 2004;Demergasso et al, 2004;Jiang et al, 2006;Maturrano et al, 2006;Mutlu et al, 2008;Pagaling et al, 2009;Sabet et al, 2009;Oh et al, 2010;Rodriguez-Brito et al, 2010). These studies confirm high abundance of a few dominant species with widespread geographical distribution, but the intermittent recovery of atypical, unconfirmed sequence fragments hints at additional, unrecognized diversity among halophilic archaea (Grant et al, 1999;Lopez-Garcia et al, 2001;Pagaling et al, 2009;Oh et al, 2010;Sime-Ngando et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

De novo metagenomic assembly reveals abundant novel major lineage of Archaea in hypersaline microbial communities

Narasingarao¹,

Podell²,

Ugalde³

et al. 2011

The ISME Journal

330

361

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This study describes reconstruction of two highly unusual archaeal genomes by de novo metagenomic assembly of multiple, deeply sequenced libraries from surface waters of Lake Tyrrell (LT), a hypersaline lake in NW Victoria, Australia. Lineage-specific probes were designed using the assembled genomes to visualize these novel archaea, which were highly abundant in the 0.1-0.8 lm size fraction of lake water samples. Gene content and inferred metabolic capabilities were highly dissimilar to all previously identified hypersaline microbial species. Distinctive characteristics included unique amino acid composition, absence of Gvp gas vesicle proteins, atypical archaeal metabolic pathways and unusually small cell size (approximately 0.6 lm diameter). Multi-locus phylogenetic analyses demonstrated that these organisms belong to a new major euryarchaeal lineage, distantly related to halophilic archaea of class Halobacteria. Consistent with these findings, we propose creation of a new archaeal class, provisionally named 'Nanohaloarchaea'. In addition to their high abundance in LT surface waters, we report the prevalence of Nanohaloarchaea in other hypersaline environments worldwide. The simultaneous discovery and genome sequencing of a novel yet ubiquitous lineage of uncultivated microorganisms demonstrates that even historically well-characterized environments can reveal unexpected diversity when analyzed by metagenomics, and advances our understanding of the ecology of hypersaline environments and the evolutionary history of the archaea.

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Characterization of halophiles isolated from solar salterns in Baja California, Mexico

Cited by 49 publications

References 67 publications

Comparative analysis of prokaryotic diversity in solar salterns in eastern Anatolia (Turkey)

Comparative analysis of prokaryotic diversity in solar salterns in eastern Anatolia (Turkey)

Isolation and Screening of Halophilic Bacteria for Production of Hydrolytic Enzymes

De novo metagenomic assembly reveals abundant novel major lineage of Archaea in hypersaline microbial communities

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