Characterization of Haemophilus paragallinarum Isolates from China

Chen, X.; Zhang, P.; Blackall, P. J.; Wang, Feng

doi:10.2307/1591690

Cited by 27 publications

(21 citation statements)

References 6 publications

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“…The properties of the isolates from Mexico were in broad agreement with similar characterization studies performed in several countries (Blackall et al, 1989(Blackall et al, , 1990b(Blackall et al, , 1994Chen et al, 1993;Terzolo et al, 1993). In the present -IV a All isolates produced acid from glucose and mannose but not from galactose, lactose, trehalose and xylose.…”

Section: Discussionsupporting

confidence: 87%

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Characterization ofHaemophilus paragallinarumisolates from Mexico

et al. 2000

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The carbohydrate fermentation, antimicrobial drug resistance and serological properties of 40 isolates of Haemophilus paragallinaru m from outbreaks of infectious coryza in Mexico are described. Four biochemical biovariants and five antimicrobial drug resistance patterns were recognized. All isolates were serotyped by the Page scheme, with 21 isolates being assigned to serogroup A, five isolates to serogroup B and 14 isolates to serogroup C.

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Section: Discussionsupporting

confidence: 87%

“…In Germany, serogroups A and B have been identified (Hinz, 1973). Only serogroup A has been reported in China (Chen et al, 1993), while only serogroup C has been found in Taiwan (Lin et al, 1995). The importance of the Page scheme arises from the type-specific immunity associated with the serogroups (Rimler et al, 1977;Kume et al, 1980).…”

Section: Resultsmentioning

confidence: 99%

Characterization ofHaemophilus paragallinarumisolates from Mexico

et al. 2000

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“…However, as all previous studies of H. paragallinaru m in China have failed to report the presence of serovar B (Chen et al, 1993;Miflin et al, 1997), we feel that the use of the B-ELISAs for serovar A and C in China is justifiable.…”

Section: Discussionmentioning

confidence: 78%

The development and application of a blocking ELISA kit for the diagnosis of infectious coryza

DeYuan¹,

Zhang²,

Gong³

et al. 2000

Avian Pathology

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A blocking enzyme-linked immunosorbent assay (B-ELISA) kit for the diagnosis of infectious coryza was developed in this study. The kit was based on a recently described blocking ELISA that uses monoclonal antibodies to achieve specificity for antibodies to either Haemophilus paragallinaru m serovar A or serovar C. The results showed that the B-ELISA kit detected 96 and 90%, respectively, of chickens vaccinated or challenged with H. paragallinaru m serovar A. When used on chickens vaccinated or challenged with H. paragallinaru m serovar C, the kit detected 77 and 40%, respectively, as positive. The majority of sera from vaccinated chickens were still positive on the serovars A and C ELISAs 4 months after vaccination. Based on pen trial data, the serovar A B-ELISA kit had a sensitivity of 95% and a specificity of 100%. The serovar C B-ELISA kit had a sensitivity of 73% and a specificity of 100%. A range of field sera was examined with the kit, generating results that correlated with the known vaccination/disease history of the flocks examined. As freeze drying the monoclonal antibodies and the conjugate had some effect on optimal working concentration, the kit used liquid solutions of these two reagents. The kit could be stored for 7 days at 37°C, 10 months at 4°C and more than 1 year at -20°C. Our results suggest that the kit would be a useful aid in the diagnosis of infectious coryza in China and other countries where H. paragallinaru m serovars A and C are the predominant or sole serovars.

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“…The disease was first recognized in the early 1980s and has been identified as a problem in a number of the provinces of the country (Chen et al, 1993). It has been estimated that over the 3-year period of 1986 to 1988, the disease caused losses of about 100 million yuan (approximately $US16.5 million at the 1996 exchange rate) (Chen et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

“…The only previous studies of which we are aware are the biochemical and serological characterization studies of Feng (1987) and Chen et al (1993). In both studies, all the isolates of H. paragallinarum examined (17 in total), were identified as Page serovar A.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of isolates ofHaemophilus paragallinarumfrom China by ribotyping

1997

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SUMMARYRibotyping profiles were determined for 12 Page serovar A isolates of Haemophilus paragallinarum from five outbreaks of infectious coryza in Hebei province in the People's Republic of China. Four enzymes were used to generate restriction digests of chromosomal DNA: HaeIII, HindIII, HpaII and SspI. The digests were probed with a digoxigenin-11-dUTP-labelled DNA probe produced by PCR amplification of the 16S rDNA of the type strain of H. paragallinarum. Only one profile was seen among the 12 isolates when using either HindIII or SspI. Three different ribotype profiles were seen with HpaII, while four different profiles were detected with HaeIII. Cluster analysis of the profiles generated by HpaII and HaeIII corresponded with the known epidemiological history of the isolates. These results are the first molecular characterization of isolates of H. paragallinarum from China and confirm the existence of genetic diversity in the H. paragallinarum population in that country.

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Characterization of Haemophilus paragallinarum Isolates from China

Abstract: The biochemical and serological properties of 11 isolates of Haemophilus paragallinarum from outbreaks of infectious coryza in the People's Republic of China are described. All 11 isolates had the typical biochemical properties of H. paragallinarum, and all belonged to Page serovar A.

Cited by 27 publications

References 6 publications

Characterization ofHaemophilus paragallinarumisolates from Mexico

Characterization ofHaemophilus paragallinarumisolates from Mexico

The development and application of a blocking ELISA kit for the diagnosis of infectious coryza

Molecular characterization of isolates ofHaemophilus paragallinarumfrom China by ribotyping

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