Characterization of gut dominant microbiota in obese patients with nonalcoholic fatty liver disease

Abstract

In obese patients, non-alcoholic fatty liver (NAFLD) is common. However, whether there is a connection between the gut microbiota and the onset of NAFLD in obese people is yet unknown. Using quantitative real-time PCR, the microbiota of feces of the eligible 181 obese individuals was identified to compare the differences in gut microbiota between obesity with NAFLD and simple obesity. According to the findings, the gut dominant microbiota was similar between obesity with NAFLD and simple obesity. Nonetheless, compared to the simple obesity group, the quantity of Faecalibacterium prausnitzii colonies was much lower in the obesity with the NAFLD group. Bacteroides were present in greater than 65% of both groups. Bacteroides, Clostridium leptum, and Clostridium butyricum accounted for more than 80% of the cases in the obesity with NAFLD group, whereas Bacteroides, Clostridium butyricum, and F. prausnitzii accounted for more than 80% of the cases in the simple obesity group. We look for potential contributing variables to obesity-related NAFLD and potential prevention measures for obese people. Based on a multi-factor logistic regression analysis, lymphocytes may be a risk factor for obesity with NAFLD while F. prausnitzii may be a protective factor. Additionally, F. prausnitzii is positively impacted by Bacteroides, Clostridium leptum, Clostridium butyricum, and Eubacterium rectale, yet adversely impacted by Enterobacteriaceae. Notably, lymphocytes and F. prausnitzii may help determine whether obese patients would develop NAFLD.

Exploring the Mediation Effect of Metabolite Levels on the Association Between Gut Microbiota and HCC: A two-step, two-sample bidirectional Mendelian Randomization

“…In a study characterizing the dominant GM in obese patients with non-alcoholic fatty liver disease, the quantity of Faecalibacterium prausnitzii colonies was significantly lower in the obesity with non-alcoholic fatty liver disease group than in the simple obesity group. Additionally, F. prausnitzii is positively impacted by C. leptum 37 , suggesting that Leptum may be a protective bacterial group, consistent with our research findings. Du and colleagues discovered that a significant enrichment of the Clostridia class can enhance antigen presentation and effector T cell function through the cGAS-STING-IFN-I pathway, thereby boosting anti-tumor immune responses 38 .…”

New Insights into the Pathogenesis of Metabolic-Associated Fatty Liver Disease (MAFLD): Gut–Liver–Heart Crosstalk

“…In obesity, gut dysbiosis is related to changes in the diversity and composition of the gut microbiota. Dysbiosis can lead to increased energy harvesting from diet, metabolic disturbances, and inflammation associated with obesity [ 39 ], as dysbiosis in obesity is defined by a decrease in beneficial bacteria and an increase in harmful bacteria [ 40 ]. Similar to that in patients with obesity, gut dysbiosis has been associated with the pathogenesis of MAFLD.…”

Bacteroides and NAFLD: pathophysiology and therapy

“…One clinical study found that the abundance of Bacteroides increased as NAFLD pathology progressed, indicating a potential association between Bacteroides and NAFLD severity ( Boursier et al, 2016 ). Conversely, a study on obese patients with NAFLD showed a significant reduction in Bacteroides abundance ( Jin and Xu, 2023 ). In another study, compared to 28 healthy controls, 39 patients with biopsy-proven NAFLD had a prominently lower abundance of Bacteroides ( Da Silva et al, 2018 ).…”