Characterization of gut bacterial community associated with worker and soldier castes of Globitermes sulphureus Haviland (Blattodea: Termitidae) using 16S rRNA metagenomic

Hussin, Nurul Akmar; Zarkasi, Kamarul Zaman; Majid, Abdul Hafiz Ab

doi:10.1016/j.aspen.2018.10.002

Cited by 12 publications

(11 citation statements)

References 37 publications

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“…For example, the genus Clostridium that is widely distributed in soils, and in the guts of humans and animals, and have also been reported at a low relative abundance in few species of weevil beetles [41, 42], has been shown to be able to ferment complex molecules including cellulose, hemicellulose, and pectin [51]. In addition, the Bacteroidales family S24–7 has been commonly found within murine gut, where it contributes to carbohydrate degradation [52], as well as within insect gut where its function has not yet been characterized [53]. Different members of Streptococcus occur mostly in human mouth and respiratory tracts, and less commonly in soils [54], but have been occasionally found in insects as potential pathogens [55], as well as in other weevil species where its function has not yet been characterized [42, 56–58].…”

Section: Discussionmentioning

confidence: 99%

Gut bacteria of weevils developing on plant roots under extreme desert conditions

et al. 2019

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BackgroundMany phytophagous insects, whose diet is generally nitrogen-poor, rely on gut bacteria to compensate for nutritional deficits. Accordingly, we hypothesized that insects in desert environments may evolve associations with gut bacteria to adapt to the extremely low nutrient availability. For this, we conducted a systematic survey of bacterial communities in the guts of weevils developing inside mud chambers affixed to plant roots in the Negev Desert of Israel, based on 16S rRNA gene amplicon sequencing.ResultsOur analyses revealed that gut bacterial communities in weevil larvae were similar across a wide geographical range, but differed significantly from those of the mud chambers and of the surrounding soils. Nevertheless, a high proportion of bacteria (including all of the core bacteria) found in the weevils were also detected in the mud chambers and soils at low relative abundances. The genus Citrobacter (of the Enterobacteriaceae family) was the predominant group in the guts of all individual weevils. The relative abundance of Citrobacter significantly decreased at the pupal and adult stages, while bacterial diversity increased. A mini literature survey revealed that members of the genus Citrobacter are associated with nitrogen fixation, recycling of uric acid nitrogen, and cellulose degradation in different insects.ConclusionsThe results suggest that although weevils could potentially acquire their gut bacteria from the soil, weevil host internal factors, rather than external environmental factors, were more important in shaping their gut bacterial communities, and suggest a major role for Citrobacter in weevil nutrition in this challenging environment. This study highlights the potential involvement of gut bacteria in the adaptation of insects to nutritional deficiencies under extreme desert conditions.

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Section: Discussionmentioning

confidence: 99%

Gut bacteria of weevils developing on plant roots under extreme desert conditions

et al. 2019

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“…Raw reads in a FastQ format were analysed using QIIME software v1.9.1 (Hussin et al, 2018) together with its essential wrapping tools. The raw reads quality was checked using FastQC v0.11.5 before further steps (Kuthoose et al, 2021).…”

Section: S Rrna Gene Amplicon Data Library and Analysismentioning

confidence: 99%

Bacterial diversity on wild shrimp post larvae in a mangrove biodiversity hotspot

Ahmad¹,

Halim²,

Nor³

et al. 2021

MJM

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Aims: This study aims to assess the impact of anthropogenic activities on shrimp microbiome in a biodiverse mangrove forest ecosystem, along the Merbok River, Kedah, Malaysia. Methodology and results: To assess the impacts, a microbiome study of wild post larvae shrimps along the river was conducted as a health indicator of the shrimp hosts which in turn would reflect the river conditions. A 16S rRNA gene amplicon sequencing of the wild post larvae shrimp microbiomes sampled across areas of varying human activities was conducted. Samples were obtained from four sites ranging from upstream river habitat to downstream brackish water towards the marine coast. Individuals detected from the sequence were then counted and their relative abundance of bacterial diversity were compared. All abundances are up to 100% and the diversity indices were calculated using proportions of each species. The Operational Taxonomy Unit (OTUs) were obtained by using USEARCH and UPARSE software. Twenty-eight bacterium phyla were detected, dominated by phyla Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes at each site. Eighteen families were dominant at each site with Streptomycetaceae being the major abundant. At the genus level, the most abundant genera were Streptomyces sp., Mesorhizobium sp., Rhizobium sp., Bacillus sp. and Pseudomonas sp. Conclusion, significance and impact of study: In general, the diversity of opportunistic and coliform bacteria was low. Thus, despite being exposed to various levels of human activities, the Merbok River and its mangrove surroundings still serve as a good spawning and nursery sites of shrimps and presumably other inhabitants.

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“…Its mound is easily identified based on a dome shape and a dark brown color (Ahmad, 1965). G. sulphureus is easily identified since the soldiers possess a bright-yellow colored body (Ab Majid & Ahmad, 2011;Hussin & Ab Majid, 2017;Hussin et al, 2018;Khizam & Ab Majid, 2019). The goal of this study is to determine the genetic diversity and phylogenetic relationship among local populations of G. sulphureus in Universiti Sains Malaysia (USM) main campus, Penang by using partial sequences of the mitochondrial COII gene.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and phylogenetic relationship of higher termite Globitermes sulphureus (Haviland)(Blattodea:Termitidae)

Hussin¹,

Majid²

2021

Sociobiology

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The subterranean higher termite Globitermes sulphureus (Blattodea: Termitidae), is a peridomestic forager and regarded as a significant pest in Southeast Asia. In this study, the populations of G. sulphureus from the USM main campus area were investigated based on partial sequences of the mitochondrial COII gene. The genetic diversity was determined using DnaSP v5 software while the phylogenetic relationship was defined using Neighbor-joining (NJ) and maximum likelihood (ML) methods using Molecular Evolutionary Genetics Analysis (MEGA 7) software. A total of 2 haplotypes were detected among the 5 sample sequences that differed by two variable sites. In addition, both phylogenetic trees gave similar topology and supporting the results from haplotype diversity. Based on the haplotype diversity and molecular phylogeny, it is proposed that geographic isolation and lack of human activities have contributed to the neutral genetic diversity of G. sulphureus.

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Characterization of gut bacterial community associated with worker and soldier castes of Globitermes sulphureus Haviland (Blattodea: Termitidae) using 16S rRNA metagenomic

Cited by 12 publications

References 37 publications

Gut bacteria of weevils developing on plant roots under extreme desert conditions

Gut bacteria of weevils developing on plant roots under extreme desert conditions

Bacterial diversity on wild shrimp post larvae in a mangrove biodiversity hotspot

Genetic diversity and phylogenetic relationship of higher termite Globitermes sulphureus (Haviland)(Blattodea:Termitidae)

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