Characterization of growth-related genes in the south-western Atlantic pink shrimp Farfantepenaeus paulensis (Pérez-Farfante 1967) through a modified DDRT-PCR protocol

Abstract

An exploratory methodology has been used to identify genes related to growth of the shrimp Farfantepenaeus paulensis (Pérez‐Farfante, 1967). Six hundred postlarvae, with an average (±SD) initial weight of 0.022 g (±0.008), were reared for 60 days in salinity 30 g L−1. In the end, the 15 heaviest (>1.2 g) and the 15 lightest (<0.6 g) animals were frozen in liquid nitrogen. A modified differential display reverse transcription‐polymerase chain reaction technique was used to generate expression profiles for all individuals. The resulting cDNA from reverse transcription were amplified by pairing 31 arbitrary primers with a reverse primer anchored to the cDNA tails. From the comparison between the expression profiles of the organisms in the two size classes, differences could be pinpointed. Bands of interest were collected, purified, cloned and sequenced. Despite the relatively small number of primers used, the methodology allowed the identification of three genes, not described previously for the investigated species and possibly related to growth. These partial sequences are likely to belong to genes that code for myosin heavy chain, cyclophilin and haemocyanin as revealed through an amino acid similarity search conducted using the blastp on‐line tool.

Genes and growth performance in crustacean species: a review of relevant genomic studies in crustaceans and other taxa

“…In a study of expression profiles in two size classes of Atlantic pink shrimp ( Farfantepenaeus paulensis ), cyclophilin, haemocyanin and myosin were strongly expressed in high weight individuals but were only weakly expressed or absent in low weight individuals (Kamimura et al . ). This result suggests that these genes may have some effect on individual growth performance, and warrants further study in crustacean species.…”

“…In south‐western Atlantic pink shrimp Farfantepenaeus paulensis , higher MHC expression was observed in a high weight shrimp group, a result that identified MHC as a possible growth candidate gene (Kamimura et al . ).…”

“…This results in muscle development in crayfish (Jirikowski et al 2010). If differences in weight and size are related to the production of muscle tissue, it is likely that myosin will be one of the genes expressed (Kamimura et al 2008). In south-western Atlantic pink shrimp Farfantepenaeus paulensis, higher MHC expression was observed in a high weight shrimp group, a result that identified MHC as a possible growth candidate gene (Kamimura et al 2008).…”

Curative impacts of squid (Loligosp.) ink extract on haemocyte, digestive enzymes andCypAgene expression of Vaname Shrimp (Litopenaeus vannamei) against white faeces syndrome (WFS)

“…This disease often occurs in shrimp farming which uses additional feed and is less likely to occur in natural ponds (Kokarkin, ). There are genes to indicate the presence of pathogens, one of them is Cyclophilin‐A ( CypA ) (Kamimura et al., 2008).…”

Shrimp farming as an alternative to artisanal fishermen communities: the Case of Patos Lagoon, Brazil

“…The accelerated search to close the life cycle and improve the reproductive performance of F. paulensis in captivity will certainly contribute to the design of an efficient breeding program. An initial attempt to determine the genes related to growth performance using the DDRT-PCR (Differential display Reverse Transcription -Polymerase Chain Reaction) has been reported (Kamimura et al, 2008).…”