Characterization of Green Pigment from Sauropus androgynus Shoot Cultures

Abstract

The dark green leaves of Sauropus androgynus (Euphorbiaceae) have various nutritive values and are commonly used for human consumption as food, medicine, and natural dye substance in South-east Asia. Shoot cultures of this plant were established by adding various concentrations of kinetin (Kn) and benzyl adenine (BA) using nodal explants. The best results were recorded when Kn 0.1 mg/L was used with BA 1 mg/L (BA1Kn0.1). Spectrophotometric analysis showed two peaks of green pigment in shoot cultures, A pigment (λmax = 663.6 - 663.8 nm, absorbance 0.1111) and B pigment (λmax= 611.3 - 613.9 nm, absorbance 0.0390). Thin Layer Chromatography (TLC) analysis showed two green spots (Rf Y = 0.31 and Rf Z = 0.25) of shoot cultures on medium supplemented with BA1Kn0.1 for 10 days. Pigment profiles of shoot culltures were similar to their corresponding mother plants. Random Amplified Polymorphic DNA (RAPD) was used as a preliminary technique to evaluate the genetic similarity of the shoot cultures and their corresponding mother plants. It showed four similar DNA banding patterns to their leaves, ranging from 271-765 bp.

Molecular characterization of red ginger varieties (Zingiber officinale Roxb. var. rubrum) by DNA markers

“…Molecular characterization of red ginger varieties (Z.officinale var. rubrum) by RAPD markers are not only useful for controlling the quality of raw materials but also for ensuring the genetic stability of herbal raw material plants cultivated using the plant tissue culture system as has been carried out in our previous research using RAPD as a preliminary technique to evaluate the genetic similarity of the shoot cultures of S. androgynus and their corresponding mother plants (Yunita et al 2021). Plant tissue culture can induce genetic and epigenetic modifications, leading to heritable alterations in phenotypes.…”