Characterization of grass carp (<i>Ctenopharyngodon idella</i>) beta-defensin 1: implications for its role in inflammation control

Yang, Kun; Hou, Boren; Ren, Fangfang; Zhou, Hong; Zhao, Taiqiang

doi:10.1080/09168451.2018.1519386

Cited by 20 publications

(5 citation statements)

References 24 publications

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“…Yang et al, 2017), so that, it is speculated that the upregulation of the intestinal def gene expression in the present study has been a response to potential intestinal dysbiosis, as stated for the intestinal lys expression. Moreover, defensins have anti‐inflammatory roles in fish (K. Yang et al, 2019), thus, the upregulation of def gene in the MPVC/Cu‐exposed fish may be a defensive response against the toxicant‐induced inflammation (evidenced by upregulation in cyp1a1 and hsp70 gene expressions).…”

Section: Discussionmentioning

confidence: 99%

Histopathological damage and stress‐ and immune‐related genes' expression in the intestine of common carp, Cyprinus carpio exposed to copper and polyvinyl chloride microparticle

et al. 2021

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The present study aimed at assessing the singular and combined effects of water copper and polyvinyl chloride microplastic (MPVC) on intestinal copper accumulation, histopathological damage, and stress‐/immune‐related genes' expression in common carp, Cyprinus carpio. Four groups of fish were maintained in triplicate: control (kept in clean water), Cu (exposed to 0.25 mg/L of copper), MPVC (exposed to 0.5 mg/L of MPVC), and Cu‐MPVC (exposed to 0.25 mg/L of copper + 0.5 mg/L of MPVC). After 14‐day exposure, the fish of Cu and Cu‐MPVC treatments exhibited significantly higher intestinal copper contents, compared to the fish of control and MPVC treatments. In this regard, the Cu‐MPVC fish had significantly higher copper content than the Cu fish. Exposure to copper and/or MPVC significantly upregulated the intestinal heat shock protein 70 (hsp70), cytochrome P450 family 1 subfamily A member 1 (cyp1a1), lysozyme (lys), defensin (def), mucin 2 (muc2), and mucin 5 (muc5) expression. The highest expression of hsp70, cyp1a1, lys, and def was related to Cu‐MPVC treatment; whereas, the highest expression of muc2 and muc5 was observed in Cu and MPVC treatments. Exposure to copper and/or MPVC induced intestinal damage, which Cu‐MPVC fish exhibited the highest severity. The present study revealed that exposure to copper and/or MPVC causes intestinal histopathological damage and upregulation in stress‐ and immune‐related genes' expression. The most serious effects were observed in Cu‐MPVC treatment that might be due to additive effects of copper and MPVC and/or higher copper accumulation in this treatment.

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Section: Discussionmentioning

confidence: 99%

Histopathological damage and stress‐ and immune‐related genes' expression in the intestine of common carp, Cyprinus carpio exposed to copper and polyvinyl chloride microparticle

et al. 2021

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“…Fish β-defensins have been reported to show anti-Gram negative bacterial activity [ 12 , 13 , 18 , 53 ], anti-Gram-positive bacterial activity [ 8 , 14 , 15 , 71 ], anti-viral activity [ 16 – 18 , 72 ], immunomodulatory and chemotactic responses [ 12 , 14 , 15 , 73 ], and anti-inflammatory roles [ 74 ]. Besides the afore-mentioned bioactivities, On -Def is also predicted to have anti-fungal and anti-cancer property.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of a novel β-defensin isoform from the red-toothed trigger fish, Odonus niger (Ruppel, 1836)

Archana

Athira

Anju

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background The concern regarding a post-antibiotic era with increasing drug resistance by pathogens imposes the need to discover alternatives for existing antibiotics. Antimicrobial peptides (AMPs) with their versatile therapeutic properties are a group of promising molecules with curative potentials. These evolutionarily conserved molecules play important roles in the innate immune system of several organisms. The β-defensins are a group of cysteine rich cationic antimicrobial peptides that play an important role in the innate immune system by their antimicrobial activity against the invading pathogens. The present study deals with a novel β-defensin isoform from the red-toothed trigger fish, Odonus niger. Total RNA was isolated from the gills, cDNA was synthesized and the β-defensin isoform obtained by polymerase chain reaction was cloned and subjected to structural and functional characterization in silico. Results A β-defensin isoform could be detected from the gill mRNA of red-toothed trigger fish, Odonus niger. The cDNA encoded a 63 amino acid peptide, β-defensin, with a 20 amino acid signal sequence followed by 43 amino acid cationic mature peptide (On-Def) having a molecular weight of 5.214 kDa and theoretical pI of 8.89. On-Def possessed six highly conserved cysteine residues forming disulfide bonds between C1–C5, C2–C4, and C3–C6, typical of β-defensins. An anionic pro-region was observed prior to the β-defensin domain within the mature peptide. Clustal alignment and phylogenetic analyses revealed On-Def as a group 2 β-defensin. Furthermore, it shared some structural similarities and functional motifs with β-defensins from other organisms. On-Def was predicted to be non-hemolytic with anti-bacterial, anti-viral, anti-fungal, anti-cancer, and immunomodulatory potential. Conclusion On-Def is the first report of a β-defensin from the red-toothed trigger fish, Odonus niger. The antimicrobial profile showed the potential for further studies as a suitable candidate for antimicrobial peptide therapeutics.

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“…The expression of the Moronecidin gene was detected in various parts of the fish including the gill, skin, intestine, spleen, anterior kidney, and blood cells using kinetic reverse transcription (RT)-PCR [ 77 ]. In grass carp, the β-defensin gene was highly expressed in the brain and liver, possibly for the regulation of immune response and inflammation within these tissues [ 170 ]. The β-defensin gene of Atlantic cod also showed increased expression in the swim bladder and peritoneum wall [ 171 ].…”

Section: Expression Modulation Of Amp Genes In Fishesmentioning

confidence: 99%

Drawing immune-capacity of fish-derived antimicrobial peptides for aquaculture industry: A comprehensive review

Debbarma,

Narsale,

Acharya

et al. 2024

Comparative Immunology Reports

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Characterization of grass carp (Ctenopharyngodon idella) beta-defensin 1: implications for its role in inflammation control

Cited by 20 publications

References 24 publications

Histopathological damage and stress‐ and immune‐related genes' expression in the intestine of common carp, Cyprinus carpio exposed to copper and polyvinyl chloride microparticle

Histopathological damage and stress‐ and immune‐related genes' expression in the intestine of common carp, Cyprinus carpio exposed to copper and polyvinyl chloride microparticle

Molecular characterization of a novel β-defensin isoform from the red-toothed trigger fish, Odonus niger (Ruppel, 1836)

Drawing immune-capacity of fish-derived antimicrobial peptides for aquaculture industry: A comprehensive review

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