Characterization of Grapevine Rootstocks using Microsatelite Markers

Dzhambazova, Teodora; Hvarleva, T.; Hadjinicoli, A.; Tsvetkov, I.; Atanassov, A.; Atanassov, Ilian

doi:10.1080/13102818.2007.10817414

Cited by 13 publications

(13 citation statements)

References 18 publications

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“…Similar findings were reported by Sefc et al (1998) who analyzed and discriminated 19 grape rootstock genotypes using microsatellite markers; while Fatahi et al (2003) for four rootstocks included in Iranian grapevine collections. Several workers also reported diversity and varietal identification in grape rootstocks using SSR markers (Lin and Walker 1998, Dzhambazova et al 2007, Anuradha et al 2007, Gizella et al 2011. Among various marker systems, the most preferential and reliable marker for genotype identification in grape was SSR due to its high level of polymorphism, abundance in the genome, co-dominant nature and Mendelian manner of inheritance.…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization and Genetic Relationships of Some Stress Tolerant Grape Rootstock Genotypes as Revealed by ISSR and SSR Markers

Motha

Singh

et al. 2018

Plant Tissue Cult. & Biotech.

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Grapevine rootstocks are a complex group of plants; most of them are interspecific hybrids evolved using parent genotypes having inherent tolerance to biotic and abiotic stresses. Fifteen species and interspecific hybrids of grape rootstocks along with three Vitis vinifera cultivars from IARI germplasm unit were analyzed with ten ISSR and seven SSR primers combinations. These ISSR produced 69 scorable bands with band size ranged from 200 to 1500 bp, PIC from 0.66 to 0.86 and primer heterozygosity ranged from 0.71 to 0.88. While, SSR primers detected a total of 24 alleles across 15 genotypes with PIC from 0.43 to 0.78 and primer heterozygosity from 0.49 to 0.81.The genetic similarity among the 15 grape genotypes using ISSR and SSR ranged from 0.27 to 1.00 in ISSR and 0.05 to 1.00 in SSR analysis. The genetic similarity matrices using ISSR analysis ranged from 27.0 to 85.0% and SSR 5.0 to 83.0%, respectively. The Vitis species Dogridge and Salt Creek had the highest similarity coefficient of 85% with ISSR analysis, while the interspecific hybrids 1103 Paulsen and 110 Richtier had the maximum similarity coefficient of 83% as identified by SSR analysis. The two marker systems formed two main clusters which were almost similar to that of PCA values. The principal coordinate analysis further helped in depicting the variability among species and hybrids of grape genotypes in three dimensional modes. In case of ISSRs, the first three coordinates accounted 51.92% for the existing variability, while with SSRs the variability accounted is 59.69%. The results revealed that ISSR and SSR markers could be exploited for genetic diversity analysis among the highly heterozygous grape rootstock species and interspecific hybrids.

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Section: Resultsmentioning

confidence: 99%

Molecular Characterization and Genetic Relationships of Some Stress Tolerant Grape Rootstock Genotypes as Revealed by ISSR and SSR Markers

Motha

Singh

et al. 2018

Plant Tissue Cult. & Biotech.

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“…However, we cannot rule out other factors like limitations of interference and convergence between similar individuals, or the presence of low gene flow at this locus. These primers can also be recommended to be used in study of species identification (Dzhambazova et al, 2007;Klein et al, 2008;Pollefeys andBousquet, 2003, This et al, 2004).…”

Section: Marker Efficiencymentioning

confidence: 99%

Comparison of genetic diversity between cultivated and wild grape varieties originating from the Near-Caspian zone of Azerbaijan

Salayeva

Decroocq

Mariette³

et al. 2010

OENO One

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Aim:The Caucasus is considered as a possible primary centre of origin of grapevine. The aim of our research work was to study genetic diversity among cultivated Caucasian grape varieties and wild relatives originating from Near-Caspian areas of Azerbaijan on the basis of simple sequence repeat (SSR) markers. Methods and Results:Microsatellite allele data obtained in 31 local table grape varieties of Vitis vinifera L. ssp. sativa (Absheron region) and 34 wild varieties of Vitis vinifera L. ssp. sylvestris (Nabran, Davachi and Guba regions) were used to estimate population genetic parameters such as the polymorphism information content (PIC), the probability of identity (PI) and the frequency of null alleles (r). The genetic differences among the wild populations and between the wild and the cultivated gene pools were assessed using POPGENE, Arlequin and GENETIX programs. The selected fifteen microsatellite (SSR) markers revealed a high degree of polymorphism within and among the grape populations analyzed. The genetic similarity index ranged from 0.02 to 0.933. Conclusions:Among the selected markers, VVMD28 and VVMD36 displayed the highest diversity level regarding the expected heterozygosity and PIC (highest values) and PI (lowest values). In consequence, we postulate that these two markers are the most appropriate ones for the identification of grape accession and the determination of genetic diversity among cultivated and wild grape genotypes. Clustering analysis based on SSR markers data led to a good separation between cultivated and wild accessions and between wild accessions originating from different regions.Significance and impact of the study: The fifteen microsatellite markers used in this study were highly informative for the identification and analysis of genetic structure of Azerbaijan grapevine populations and clarified the relationships among grape accessions.Objectif: Le Caucase est une zone géographique considérée comme un centre de diversité de V. vinifera. Le but de cette étude était d'étudier, au moyen de marqueurs microsatellites, la diversité génétique des variétés locales de vigne du Caucase, ainsi que celle des populations sauvages qui poussent en Azerbaïdjan, dans les régions proches de la mer Caspienne. Méthode et résultats:En se basant sur ces données microsatellites obtenues sur 31 variétés locales de raisin de table et 34 individus sauvages appartenant à l'espèce V. vinifera, nous avons pu estimer différents paramètres tels que le PIC (polymorphic information content), la fréquence d'allèles nuls (r), le PI (probabilité d'identité). Pour estimer la diversité génétique à l'intérieur des compartiments sauvages et entre les compartiments sauvages et cultivés, nous avons utilisé les logiciels POPGENE, Arlequin et GENETIX. Nous avons pu montrer que les 15 marqueurs microsatellites avaient un niveau élevé de polymorphisme à l'intérieur et entre les populations analysées. L'index de similarité génétique variait entre 0,02 et 0,933. Conclusions:Les marqueurs VVMD28 et VVMD36 ont révélé le plu...

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“…Ampelography and ampelometry based on morphological differences between the varieties are known methods for varietal identification. A lot of molecular markers have been employed to characterise grape varieties and rootstocks (Dzhambazova et al, 2007). As the physiology and biochemistry of rootstocks vary under similar sets of management practices, the physiology and biochemical composition of the mother vines play an important role in the propagation, growth and development of the vine, water-use efficiency, pest and disease tolerance, quality of the grapes, etc.…”

Section: Introductionmentioning

confidence: 99%

Physiological and Biochemical Characterisation of Grape Rootstocks

Satisha¹,

Ramteke²,

Karibasappa³

2016

SAJEV

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Ten grape rootstocks were characterised for their various morphological, physiological and biochemical parameters. Significant differences were observed for most of the parameters studied. Rootstocks in the group of Vitis berlandierii × Vitis rupestris, such as 110 R, 1103 P, 99 R and B2-56, had a significantly higher content of total phenols, flavon-3-ols, flaveonoids, proline and total protein. Optimum C/N ratios in the mother vines of these rootstocks helped attain better rooting percentages of hardwood cuttings. Rootstocks such as Dog Ridge, 110 R, B2-56 and Salt Creek recorded the highest relative water content (RWC) and water-use efficiency (WUE) at single-leaf level, which is an important mechanism in overcoming drought tolerance. The high phenolic content in rootstocks such as Dog Ridge, 99 R, 110R and 1103 P may help in reducing the incidence of major grape diseases in commercial table varieties if grafted onto these rootstocks. This was established in our earlier studies of the incidence of powdery mildew in Thompson Seedless grafted onto different rootstocks. A significant and positive correlation was observed for WUE and total protein and proline content. Thus, the physio-biochemical characterisation of rootstocks may help to identify particular rootstocks that could influence a desired trait in commercial table / wine grape varieties after grafting.

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Characterization of Grapevine Rootstocks using Microsatelite Markers

Cited by 13 publications

References 18 publications

Molecular Characterization and Genetic Relationships of Some Stress Tolerant Grape Rootstock Genotypes as Revealed by ISSR and SSR Markers

Molecular Characterization and Genetic Relationships of Some Stress Tolerant Grape Rootstock Genotypes as Revealed by ISSR and SSR Markers

Comparison of genetic diversity between cultivated and wild grape varieties originating from the Near-Caspian zone of Azerbaijan

Physiological and Biochemical Characterisation of Grape Rootstocks

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