Characterization of Gold Nanorods Conjugated with Synthetic Glycopolymers Using an Analytical Approach Based on spICP-SFMS and EAF4-MALS

Abstract

A new comprehensive analytical approach based on single-particle inductively coupled plasma-sector field mass spectrometry (spICP-SFMS) and electrical asymmetric-flow field-flow-fractionation combined with multi-angle light scattering detection (EAF4-MALS) has been examined for the characterization of galactosamine-terminated poly(N-hydroxyethyl acrylamide)-coated gold nanorods (GNRs) in two different degrees of polymerization (DP) by tuning the feed ratio (short: DP 35; long: DP 60). spICP-SFMS provided information on the particle number concentration, size and size distribution of the GNRs, and was found to be useful as an orthogonal method for fast characterization of GNRs. Glycoconjugated GNRs were separated and characterized via EAF4-MALS in terms of their size and charge and compared to the bare GNRs. In contrast to spICP-SFMS, EAF4-MALS was also able of providing an estimate of the thickness of the glycopolymer coating on the GNRs surface.

A Nanoplasmonic Assay for Point-of-Care Detection of Mannose-Binding Lectin in Human Serum

“…UV–vis spectroscopy revealed a red shift of the longitudinal LSPR band for the glyco-coated GNRs, ζ-potential indicated a decrease of negative surface charge, and TEM showed that the nanorod dimensions were retained during coating, while DCS and NTA analyses indicated an increase in particle size compared with the citrate-GNRs, as expected (see Table ). When nanoparticles are coated and therefore the resulting density and size are changed, their size measured by DCS is altered, showing here a shift toward apparent smaller diameters. , Altogether, these data confirmed the successful attachment of the glyco ligands to the particle surface and their stability in a colloidal suspension.…”

“…When nanoparticles are coated and therefore the resulting density and size are changed, their size measured by DCS is altered, showing here a shift toward apparent smaller diameters. 40,41 Altogether, these data confirmed the successful attachment of the glyco ligands to the particle surface and their stability in a colloidal suspension.…”

Electrical asymmetric-flow field-flow fractionation with a multi-detector array platform for the characterization of metallic nanoparticles with different coatings

“…EAF4 is still in its infancy and the scarce studies carried out so far to optimize the carrier for the determination of µ and/or ζ are limited to exosomes, liposomes, proteins, and polystyrene latex particles [15,17,18]. Other authors have used EAF4 for Au nanorods recently, but only to confirm that those NPs carry negative charge and thereby confirming the ζ obtained by a separate instrument [19].…”

Selected Papers from the Second International Online Conference on Nanomaterials

“…Thus, four main categories can be distinguished based on their nature [ 1 ]: (1) carbon-based nanomaterials, which are found in morphologies such as ellipsoids or spheres, hollow tubes, or ultrathin sheets; fullerenes (C 60 ), carbon nanotubes (CNTs), carbon nanofibers, carbon black, graphene (G), and its derivatives graphene oxide (GO) and reduced graphene oxide (rGO) are in this category [ 2 , 3 ]. (2) Inorganic-based nanomaterials, which include metal [ 4 ] and metal oxide nanoparticles, such as TiO 2 , SnO and ZnO [ 5 , 6 , 7 ], and semiconductors, such as silicon, III-V compounds, transition metal dichalcogenides, Xenes, etc. [ 8 ].…”

“…The articles present very different types of nanomaterials, such as gold nanorods [ 4 ], WTe 2 nanocrystals [ 11 ], CaCO 3 nanoparticles [ 12 ], ferromagnetic nanoparticles [ 13 ], CuO nanocrystals and single-walled carbon nanotubes [ 14 ], graphene [ 3 ], and GO derivatives [ 15 ].…”