Characterization of glycoprotein E C-End of West Nile virus and evaluation of its interaction force with αVβ3 integrin as putative cellular receptor

Bogachek, Maria V.; Зайцев, Б. Н.; Sekatskiǐ, S. K.; Протопопова, Е. В.; Va, Ternovoĭ; Ivanova, Alla V.; Kachko, Alla; Иванисенко, В. А.; Dietler, Giovanni; Loktev, V. B.

doi:10.1134/s0006297910040115

Cited by 16 publications

(21 citation statements)

References 36 publications

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“…24 In the present study, similar mechanisms could underlie the association between blood group A and symptomatic WNV disease. Indeed, it was shown that WNV interacts with LBP and αVβ3 integrin at the cell surface of host cells through its E glycoprotein, 47, 48 and blood group A antigen could serve as a co-receptor for WNV. This would explain the findings that subjects with blood group A demonstrated higher viral loads in whole blood than subjects with other blood groups, 14 however, this would not explain why the same assoication is not observed for subjects with blood group AB, whose erythrocytes express approximately half of the number of A sites than A erythrocytes 49 and this would not explain the association with Rh(D)-negativity.…”

Section: Discussionmentioning

confidence: 99%

Blood group A and D negativity are associated with symptomatic West Nile virus infection

et al. 2016

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Background West Nile virus (WNV) infection is mostly asymptomatic but 20% of subjects report WNV fever and 1% of patients experience neurological diseases with higher rates in elderly and immunosuppressed persons. With no treatment and no vaccine to prevent the development of symptomatic infections, it is essential to understand prognostic factors influencing symptomatic disease outcome. Host genetic background has been linked to the development of WNV neuroinvasive disease. The present study investigates the association between the ABO and Rh(D) blood group status and WNV disease outcome. Study Design and Methods The distribution of blood groups was investigated within a cohort of 374 WNV+ blood donors including 244 asymptomatic (AS) and 130 symptomatic (S) WNV+ blood donors. Logistic regression analyses were used to examine associations between A, B, O and Rh(D) blood groups and WNV clinical disease outcome. Results Symptomatic WNV+ donors exhibited increased frequencies of blood group A (S 47.6% AS 36.8%, P=0.04, OR [95%CI] 1.56 [1.01–2.40]) and Rh(D)-negative individuals (S 21.5% AS 13.1%, P=0.03, OR [95%CI] 1.82 [1.04–3.18]). Conclusion The findings suggest a genetic susceptibility placing blood group A and Rh(D)-negative individuals at risk for the development of symptomatic disease outcome after WNV infection.

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Section: Discussionmentioning

confidence: 99%

Blood group A and D negativity are associated with symptomatic West Nile virus infection

et al. 2016

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“…It has been reported that host surface glycoproteins interact with the viral envelope proteins to initiate attachment (Chen et al, 1997b; Kroschewski et al, 2003; Davis et al, 2006). Attachment might also be mediated by integrins, cytoskeleton proteins, and cholesterol-dependent lipid raft pathways (Medigeshi et al, 2008; Bogachek et al, 2010). Internalization is then mediated by clathrin-coated vesicles (Chu and Ng, 2004).…”

Section: The Flavivirus Infectious Cyclementioning

confidence: 99%

Functional Information Stored in the Conserved Structural RNA Domains of Flavivirus Genomes

et al. 2017

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The genus Flavivirus comprises a large number of small, positive-sense single-stranded, RNA viruses able to replicate in the cytoplasm of certain arthropod and/or vertebrate host cells. The genus, which has some 70 member species, includes a number of emerging and re-emerging pathogens responsible for outbreaks of human disease around the world, such as the West Nile, dengue, Zika, yellow fever, Japanese encephalitis, St. Louis encephalitis, and tick-borne encephalitis viruses. Like other RNA viruses, flaviviruses have a compact RNA genome that efficiently stores all the information required for the completion of the infectious cycle. The efficiency of this storage system is attributable to supracoding elements, i.e., discrete, structural units with essential functions. This information storage system overlaps and complements the protein coding sequence and is highly conserved across the genus. It therefore offers interesting potential targets for novel therapeutic strategies. This review summarizes our knowledge of the features of flavivirus genome functional RNA domains. It also provides a brief overview of the main achievements reported in the design of antiviral nucleic acid-based drugs targeting functional genomic RNA elements.

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“…Cellular α v β 3 integrin and laminin-binding protein were reported to function as WNV receptors [17,18] but the use of α v β 3 integrin as a WNV receptor was not consistently supported by data from subsequent studies. Even though several flaviviruses including WNV contain an integrin recognition RGD/RGE motif within the domain III region of their E proteins, RGD peptides did not competitively inhibit WNV entry [17].…”

Section: Virion Morphology Attachment and Entrymentioning

confidence: 99%

Replication Cycle and Molecular Biology of the West Nile Virus

Brinton

2013

Viruses

126

128

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West Nile virus (WNV) is a member of the genus Flavivirus in the family Flaviviridae. Flaviviruses replicate in the cytoplasm of infected cells and modify the host cell environment. Although much has been learned about virion structure and virion-endosomal membrane fusion, the cell receptor(s) used have not been definitively identified and little is known about the early stages of the virus replication cycle. Members of the genus Flavivirus differ from members of the two other genera of the family by the lack of a genomic internal ribosomal entry sequence and the creation of invaginations in the ER membrane rather than double-membrane vesicles that are used as the sites of exponential genome synthesis. The WNV genome 3' and 5' sequences that form the long distance RNA-RNA interaction required for minus strand initiation have been identified and contact sites on the 5' RNA stem loop for NS5 have been mapped. Structures obtained for many of the viral proteins have provided information relevant to their functions. Viral nonstructural protein interactions are complex and some may occur only in infected cells. Although interactions between many cellular proteins and virus components have been identified, the functions of most of these interactions have not been delineated.

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Characterization of glycoprotein E C-End of West Nile virus and evaluation of its interaction force with αVβ3 integrin as putative cellular receptor

Cited by 16 publications

References 36 publications

Blood group A and D negativity are associated with symptomatic West Nile virus infection

Blood group A and D negativity are associated with symptomatic West Nile virus infection

Functional Information Stored in the Conserved Structural RNA Domains of Flavivirus Genomes

Replication Cycle and Molecular Biology of the West Nile Virus

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