Characterization of glycation in an IgG1 by capillary electrophoresis sodium dodecyl sulfate and mass spectrometry

Solid-State mAbs and ADCs Subjected to Heat-Stress Stability Conditions can be Covalently Modified with Buffer and Excipient Molecules

“…Our observations regarding the modification of proteins through condensation with trehalose and sucrose are unrelated to previously documented occurrences of non‐enzymatic glycation . In our findings, we observed extensive disaccharide adducts on lyophilized, heat‐stressed proteins that were formulated with trehalose and sucrose.…”

Rapid and multi-level characterization of trastuzumab using sheathless capillary electrophoresis-tandem mass spectrometry

“…The methods used should cover a wide range of structural information, e.g., amino acid sequence, disulfide bonds, glycosylation, posttranslational modifications (PTM) 8 . Additional to MS, mAbs characterization requires a panel of orthogonal methods to cover a full range of structural information, 9 including reverse phase liquid chromatography (RP-LC), 10 - 12 ion exchange chromatography (IEC), size exclusion chromatography (SEC), 13 capillary isoelectric focusing (cIEF) 14 or micellar electrokinetic chromatography (MEKC) 15 , 16 …”

Rapid evaluation for heterogeneities in monoclonal antibodies by liquid chromatography/mass spectrometry with a column-switching system

“…2). Heterogeneity analyses of L chain and H chain produced by off-line reduction of mAbs were reported previously, but on-line reduction methods for mAbs have not been reported [13,[20][21][22][23]. When we analyzed reduced rituximab by LC/MS system 2 without separation process using the PLRP-S column, we were not able to obtain sufficient deconvoluted mass spectra of the L chain and H chain.…”