Characterization of glutathione-S-transferases in zebrafish (Danio rerio)

Glisic, Branka; Mihaljević, Ivan; Popović, Marta; Žaja, Roko; Lončar, Jovica; Fent, Karl; Kovačević, Radmila; Smital, Tvrtko

doi:10.1016/j.aquatox.2014.10.013

Cited by 114 publications

(68 citation statements)

References 64 publications

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“…CYP3a65 of zebrafish is an ortholog of CYP3a and is expressed mainly in the liver and intestine 29 . MGST3b also has an important role in the biotransformation of xenobiotics 30 . Down-regulation of CYP3a65 and MGST3b in GIFT liver 24 h post-heat stress, suggests that the detoxification of xenobiotic substances may have been impaired 28 .…”

Section: Discussionmentioning

confidence: 99%

The expression profiles of miRNA–mRNA of early response in genetically improved farmed tilapia (Oreochromis niloticus) liver by acute heat stress

Qiang

Bao

Tao

et al. 2017

Sci Rep

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Genetically improved farmed tilapia (GIFT, Oreochromis niloticus) are commercially important fish that are cultured in China. GIFT are highly susceptible to diseases when exposed to high temperatures in summer. Better understanding the GIFT regulatory response to heat stress will not only help in determining the relationship between heat stress signalling pathways and adaption mechanisms, but will also contribute to breeding new high-temperature tolerant strains of GIFT. In this study, we built control (28 °C) and heat-treated (37.5 °C) groups, and extracted RNA from the liver tissues for high-throughput next-generation sequencing to study the miRNA and mRNA expression profiles. We identified 28 differentially expressed (DE) miRNAs and 744 DE mRNAs between the control and heat-treated groups and annotated them using the KEGG database. A total of 38 target genes were predicted for 21 of the DE miRNAs, including 64 negative miRNA–mRNA interactions. We verified 15 DE miRNA–mRNA pairs and 16 other DE mRNAs by quantitative real-time PCR. Important regulatory pathways involved in the early response of GIFT to heat stress included organism system, metabolism, and diseases. Our findings will facilitate the understanding of regulatory pathways affected by acute heat stress, which will help to better prevent heat damage to GIFT.

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Section: Discussionmentioning

confidence: 99%

The expression profiles of miRNA–mRNA of early response in genetically improved farmed tilapia (Oreochromis niloticus) liver by acute heat stress

Qiang

Bao

Tao

et al. 2017

Sci Rep

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“…Two ligand binding sites were present in GST. The G-sites were highly specific for glutathione binding sites and contained 11 highly conserved amino acids: Tyr 7 , Arg 13 , Trp 38 , Lys 46 , Gln 53 , Leu 54 Pro 55 , Gln 66 , Ser 67 , Glu 98 and Asp 99 (alignment positions in GST of M. nipponense 7, 11, 46, 50, 59, 60, 61, 72, 73, 101 and 102, respectively) [ 33 , 34 ]. The second ligand binding site, H-sites, interacting with electrophilic xenobiotic substrates, contained eight conserved residues: Tyr 7 , Phe 8 , Val 10 , Arg 13 , Val 104 , Tyr 108 , Asn 204 and Gly 205 [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Activity and Transcriptional Responses of Hepatopancreatic Biotransformation and Antioxidant Enzymes in the Oriental River Prawn Macrobrachium nipponense Exposed to Microcystin-LR

Yuan

Wang

et al. 2015

Toxins

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Microcystins (MCs) are a major group of cyanotoxins with side effects in many organisms; thus, compounds in this group are recognized as potent stressors and health hazards in aquatic ecosystems. In order to assess the toxicity of MCs and detoxification mechanism of freshwater shrimp Macrobrachium nipponense, the full-length cDNAs of the glutathione S-transferase (gst) and catalase (cat) genes were isolated from the hepatopancreas. The transcription level and activity changes in the biotransformation enzyme (glutathione S-transferase (GST)) and antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx)) in the hepatopancreas of M. nipponense exposed to MC-LR (0.2, 1, 5, and 25 μg/L) for 12, 24, 72 and 96 h were analyzed. The results showed that the isolated full-length cDNAs of cat and gst genes from M. nipponense displayed a high similarity to other crustaceans, and their mRNAs were mainly expressed in the hepatopancreas. MC-LR caused significant increase of GST activity following 48–96 h (p < 0.05) and an increase in SOD activity especially in 24- and 48-h exposures. CAT activity was activated when exposed to MC-LR in 12-, 24- and 48-h exposures and then it was inhibited at 96-h exposure. There was no significant effect on GPx activity after the 12- and 24-h exposures, whereas it was significantly stimulated after the 72- and 96-h exposures (p < 0.05). The transcription was altered similarly to enzyme activity, but the transcriptional response was generally more immediate and had greater amplitude than enzymatic response, particularly for GST. All of the results suggested that MC-LR can induce antioxidative modulation variations in M. nipponense hepatopancreas in order to eliminate oxidative damage.

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“…Oviparous fish similarly deposit vitellogenin and phosvitin, major yolk proteins that scavenge free radicals attributable to phosphoserine protein structure and ironchelating capacity (Hu et al 2015). Redox homeostasis critically hinges on GPXs and glutathione de novo synthesis, demonstrated by localization of the enzymes in the yolk and increasing expression in critical tissues such as the brain, liver, and kidney during development (Glisic et al 2015;Mendieta-Serrano et al 2015). Thus, the egg is protected by a wealth of antioxidants and iron regulatory mechanisms to reduce the harmful effects of oxidative stress in embryogenesis, some of which may involve ferroptosis and lipid peroxidation.…”

Section: Protective Anti-ferroptotic Adaptations In the Eggmentioning

confidence: 99%

“…Inhibition of these thiol-containing molecules induces histological damage and the lipid peroxidation marker malondialdehyde (MDA) in zebrafish gonads (Zhang et al 2016). Thiol-containing antioxidant protein production is important in maintaining cellular redox balance and lipid health from embryo to adult but may not be sufficient (Glisic et al 2015). Nuclear factor erythroid 2-related factor (Nrf2) signaling up-regulates cellular antioxidant capacity through glutathione-related gene expression but was unable to mitigate the oxidative stress associated with transportation or heat stress in brains from production poultry (Chowdhury et al 2014;Ge et al 2017).…”

Section: Glutathione and Glutathione-dependent Peroxidasesmentioning

confidence: 99%

Regulation of lipid peroxidation and ferroptosis in diverse species

et al. 2018

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Lipid peroxidation is the process by which oxygen combines with lipids to generate lipid hydroperoxides via intermediate formation of peroxyl radicals. Vitamin E and coenzyme Q react with peroxyl radicals to yield peroxides, and then these oxidized lipid species can be detoxified by glutathione and glutathione peroxidase 4 (GPX4) and other components of the cellular antioxidant defense network. Ferroptosis is a form of regulated nonapoptotic cell death involving overwhelming iron-dependent lipid peroxidation. Here, we review the functions and regulation of lipid peroxidation, ferroptosis, and the antioxidant network in diverse species, including humans, other mammals and vertebrates, plants, invertebrates, yeast, bacteria, and archaea. We also discuss the potential evolutionary roles of lipid peroxidation and ferroptosis.

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Characterization of glutathione-S-transferases in zebrafish (Danio rerio)

Cited by 114 publications

References 64 publications

The expression profiles of miRNA–mRNA of early response in genetically improved farmed tilapia (Oreochromis niloticus) liver by acute heat stress

The expression profiles of miRNA–mRNA of early response in genetically improved farmed tilapia (Oreochromis niloticus) liver by acute heat stress

Activity and Transcriptional Responses of Hepatopancreatic Biotransformation and Antioxidant Enzymes in the Oriental River Prawn Macrobrachium nipponense Exposed to Microcystin-LR

Regulation of lipid peroxidation and ferroptosis in diverse species

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