Characterization of Glucansucrases and Fructansucrases Produced by Wild Strains<i>Leuconostoc Mesenteroides</i>URE13 and<i>Leuconostoc Mesenteroides</i>LM17 Grown on Glucose or Fructose Medium as a Sole Carbon Source

Bivolarski, Veselin; Vasileva, Tonka; Dzhambazov, Balik; Momchilova, Albena; Chobert, Jean‐Marc; Иванова, И.; Iliev, Ilia

doi:10.5504/bbeq.2013.0017

Cited by 6 publications

(9 citation statements)

References 52 publications

(72 reference statements)

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“…The fact that the extracted dextran molecules structurally resembled commercial dextran (derived from L. mesenteroides B-512F) with some modifications in the branching distribution (see Table S4) provides more support for the commercial potential of L. mesenteroides YL48. Second, some of the strains in our homology analysis have been researched in the context of commercial and novel dextran synthesis, for instance, L. mesenteroides CMG713 (40) and L. mesenteroides URE-13 (41). If the synthesis of biotechnologically important dextrans can be facilitated by combining high CO 2 levels in the large-scale fermentation process, this would have beneficial implications for a variety of biotechnological industries.…”

Section: Discussionmentioning

confidence: 99%

High Levels of CO ₂ Induce Spoilage by Leuconostoc mesenteroides by Upregulating Dextran Synthesis Genes

Dror

Savidor

Salam

et al. 2019

Appl Environ Microbiol

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During nonventilated storage of carrots, CO2gradually accumulates to high levels and causes modifications in the carrot's microbiome toward dominance ofLactobacillalesandEnterobacteriales. The lactic acid bacteriumLeuconostoc mesenteroidessecretes a slimy exudate over the surface of the carrots. The objective of this study was to characterize the slime components and the potential cause for its secretion under high CO2levels. A proteomic analysis of the exudate revealed bacterial glucosyltransferases as the main proteins, specifically, dextransucrase. A chemical analysis of the exudate revealed high levels of dextran and several simple sugars. The exudate volume and dextran amount were significantly higher whenL. mesenteroideswas incubated under high CO2levels than when incubated in an aerated environment. The treatment of carrot medium plates with commercial dextransucrase or exudate protein extract resulted in similar sugar profiles and dextran production. Transcriptome analysis demonstrated that dextran production is related to the upregulation of theL. mesenteroidesdextransucrase-encoding genesdsrDanddsrTduring the first 4 to 8 h of exposure to high CO2levels compared to aerated conditions. A phylogenetic analysis ofL. mesenteroidesYL48dsrDrevealed a high similarity to otherdsrgenes harbored by differentLeuconostocspecies. The ecological benefit of dextran production under elevated CO2requires further investigation. However, this study implies an overlooked role of CO2in the physiology and fitness ofL. mesenteroidesin stored carrots, and perhaps in other food items, during storage under nonventilated conditions.IMPORTANCEThe bacteriumLeuconostoc mesenteroidesis known to cause spoilage of different types of foods by secreting a slimy fluid that damages the quality and appearance of the produce. Here, we identified a potential mechanism by which high levels of CO2affect the spoilage caused by this bacterium by upregulating dextran synthesis genes. These results have broader implications for the study of the physiology, degradation ability, and potential biotechnological applications ofLeuconostoc.

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Section: Discussionmentioning

confidence: 99%

High Levels of CO ₂ Induce Spoilage by Leuconostoc mesenteroides by Upregulating Dextran Synthesis Genes

Dror

Savidor

Salam

et al. 2019

Appl Environ Microbiol

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“…The sucrases are sucrose inducible, soluble or cell-wall bound enzymes that synthesize HoPS in the extracellular matrix (Bivolarski et al . 2013 ). This is different from the Wzy-dependent pathway for the production of HePS, which occurs intracellularly, and the repeating units are flipped to the outside the cell and polymerized into a growing HePS chain.…”

Section: Introductionmentioning

confidence: 99%

Screening for texturing Leuconostoc and genomics behind polysaccharide production

Poulsen

Koza

Al-Nakeeb

et al. 2020

FEMS Microbiology Letters

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Synthesis of polysaccharides by Leuconostoc can result in improved texture of fermented products. A total of 249 Leuconostoc strains were screened for homo-polysaccharide production and for texturing capabilities in milk. Six Ln. mesenteroides strains with superior texturing properties had the genetic blueprint for both homo- (HoPS) and hetero-polysaccharide (HePS) synthesis. Only one strain produced texture in milk without added sucrose, suggesting HePS synthesis via the Wzy dependent pathway. In milk acidification experiments with added sucrose, all six strains depleted the sucrose and released fructose. Thus, they can be used for both texture and possibly also for sweetness enhancement.

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“…[ 35 ] The dextransucrase from this complex was purified by single-step chromatography technique. [ 32 ] The influence of different concentrations DMSO on the activity of the glycosyltransferase complex and purified dextransucrase was studied. This step is important because the terpenoids have only poor water solubility and usage of compatible organic solvents such as DMSO is required.…”

Section: Resultsmentioning

confidence: 99%

“…An extracellular dextransucrase from L. mesenteroides URE 13, cultivated on sucrose media, was purified by size-exclusion chromatography with XK 16/70 column and Sepharose CL-6B medium as previously described. [ 32 ]…”

Section: Methodsmentioning

confidence: 99%

Influence of terpenoids and acarbose on glycosyltransferases produced by strainLeuconostoc mesenteroidesURE 13

Bivolarski

Vasileva

Bozov

et al. 2014

Biotechnology & Biotechnological Equipment

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A study of the influence of different plant terpenoids and amino sugar derivate acarbose on the activity of glycosyltransferase complex and purified dextransucrase from Leuconostoc mesenteroides URE 13 strain was carried out. All the tested terpenoids showed an inhibitory effect on glycosyltransferases from strain URE 13 at concentration 0.34 mmol. Out of all studied diterpenoids splendidin showed the strongest inhibitory effect decreasing the activity of both glycosyltransferase complex and dextransucrase with 70% and 90%, respectively. The triterpenoid ursolic showed the second strongest inhibitory effect as the enzyme complex and dextransucrase from strain URE 13 retain 27% and 13% of their initial enzyme activity. Despite the higher degree of inhibition of purified dextransucrase, compared to the enzyme complex, a complete inhibition of the enzyme was not observed at the highest used terpenoid concentration (3.42 mmol). When acarbose was used as an inhibitor, a complete inhibition of dextransucrase was observed at concentration of 6.9 mmol, while the enzyme complex retained 8% of its enzyme activity. Ki values of 0.28 mmol for splendidin, 0.37 mmol for ursolic acid and 0.29 mmol for acarbose were determined from the kinetic studies of purified dextransucrase.

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Characterization of Glucansucrases and Fructansucrases Produced by Wild StrainsLeuconostoc MesenteroidesURE13 andLeuconostoc MesenteroidesLM17 Grown on Glucose or Fructose Medium as a Sole Carbon Source

Cited by 6 publications

References 52 publications

High Levels of CO ₂ Induce Spoilage by Leuconostoc mesenteroides by Upregulating Dextran Synthesis Genes

High Levels of CO ₂ Induce Spoilage by Leuconostoc mesenteroides by Upregulating Dextran Synthesis Genes

Screening for texturing Leuconostoc and genomics behind polysaccharide production

Influence of terpenoids and acarbose on glycosyltransferases produced by strainLeuconostoc mesenteroidesURE 13

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Characterization of Glucansucrases and Fructansucrases Produced by Wild StrainsLeuconostoc MesenteroidesURE13 andLeuconostoc MesenteroidesLM17 Grown on Glucose or Fructose Medium as a Sole Carbon Source

Cited by 6 publications

References 52 publications

High Levels of CO 2 Induce Spoilage by Leuconostoc mesenteroides by Upregulating Dextran Synthesis Genes

High Levels of CO 2 Induce Spoilage by Leuconostoc mesenteroides by Upregulating Dextran Synthesis Genes

Screening for texturing Leuconostoc and genomics behind polysaccharide production

Influence of terpenoids and acarbose on glycosyltransferases produced by strainLeuconostoc mesenteroidesURE 13

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High Levels of CO ₂ Induce Spoilage by Leuconostoc mesenteroides by Upregulating Dextran Synthesis Genes

High Levels of CO ₂ Induce Spoilage by Leuconostoc mesenteroides by Upregulating Dextran Synthesis Genes