Characterization of glioma stem-like cells from human glioblastomas

Yamamuro, Shun; Okamoto, Yutaka; Sano, Emiko; Ochiai, Yushi; Ogino, Akiyoshi; Ohta, Takashi; Hara, Hiroyuki; Ueda, Takuya; Nakayama, Tomohiro; Yoshino, Atsuo; Katayama, Yoichi

doi:10.3892/ijo.2015.2992

Cited by 23 publications

(16 citation statements)

References 31 publications

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“…In human glioblastomas, Son et al38 have found that CD15 is an enrichment marker of stem cells in CD133 − negative tumors. It is often used as a marker of glioma stem cell now 39. In our study, we observed higher numbers of CD133 + , nestin + , and CD15 + cells in the foci with high Cho/Cr ratios relative to those in low Cho/Cr foci.…”

Section: Discussionsupporting

confidence: 63%

Multivoxel magnetic resonance spectroscopy identifies enriched foci of cancer stem-like cells in high-grade gliomas

Qiu

Wang

et al. 2017

OTT

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ObjectiveThis study investigated the correlation between choline/creatine (Cho/Cr) ratios determined by multivoxel proton magnetic resonance spectroscopy (1H-MRS) and the distribution of cancer stem-like cells (CSLCs) in high-grade gliomas.Patients and methodsSixteen patients with high-grade gliomas were recruited and underwent 1H-MRS examination before surgery to identify distinct tumor regions with variable Cho/Cr ratios. Using intraoperative neuronavigation, tumor tissues were accurately sampled from regions with high and low Cho/Cr ratios within each tumor. The distribution of CSLCs in samples from glioma tissue regions with different Cho/Cr ratios was quantified by neurosphere culture, immunohistochemistry, and Western blot.ResultsThe mean neurosphere formation rate in tissues with high Cho/Cr ratios was significantly increased compared with that in low Cho/Cr ratio tissues (13.94±5.94 per 100 cells vs 8.04±3.99 per 100 cells, P<0.001). Immunohistochemistry indicated that tissues with high Cho/Cr ratios had elevated expression of CD133, nestin, and CD15, relative to low Cho/Cr ratio tissue samples (23.6%±3.8% vs 18.3%±3.3%, 25.2%±4.5% vs 19.8%±2.8%, 24.5%±3.8% vs 17.8%±2.2%, respectively; all P<0.001). Western blot demonstrated that relative CD133 and nestin protein expression in high Cho/Cr ratio regions was significantly higher than that in low Cho/Cr ratio tissue samples (0.50±0.17 vs 0.30±0.08, 0.45±0.13 vs 0.27±0.07, respectively; both P<0.001). The protein expression levels of CD133 and nestin were highly correlated with Cho/Cr ratios (r=0.897 and r=0.861, respectively).ConclusionCho/Cr ratios correlate with the distribution of CSLCs in high-grade gliomas, and this may assist in identifying foci enriched with CSLCs and thus improve the management of high-grade gliomas.

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Section: Discussionsupporting

confidence: 63%

Multivoxel magnetic resonance spectroscopy identifies enriched foci of cancer stem-like cells in high-grade gliomas

Qiu

Wang

et al. 2017

OTT

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“…Previous studies demonstrated that LSCs express CD133. CD133 has been used for cancer stem cell identification in several types of cancer, including glioblastoma ( 36 ), melanoma ( 37 ), liver cancer ( 38 ), osteosarcoma ( 39 ) and colon cancer ( 40 ). There are numerous studies on the expression of CD133 in acute leukemia, however, a few studies on the expression of CD133 in ALL, particularly in pediatric ALL, are contradictory.…”

Section: Discussionmentioning

confidence: 99%

Aberrant expression of CD133 and CD82 in patients with pediatric acute lymphoblastic leukemia and the clinical significance

Chen

Dai

et al. 2017

Oncol Lett

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Cluster of differentiation (CD)133 is considered to be a marker of leukemia stem cells (LSCs), which are one of the primary causes of occurrence, drug resistance and relapse of acute lymphoblastic leukemia (ALL). CD82, an adhesion molecule, performs an important role in the interaction between LSCs and their niche. The purpose of the present study was to assess CD133 and CD82 expression in patients with pediatric ALL, and to evaluate the association with the clinical data. Using flow cytometric assessment and reverse transcription-polymerase chain reaction, CD133 and CD82 expression levels were measured in the bone marrow (BM) of 37 patients with newly diagnosed (ND) pediatric ALL [ALL-ND; 30 B-cell-ALL (B-ALL) and 7 T-cell-ALL (T-ALL)], in 22 patients with complete remission pediatric ALL (ALL-CR) and in 16 age-matched children without BM disease. BM plasma CD82 concentrations were measured by ELISA. The CD82 mRNA expression level in the patients with ALL-ND was significantly higher compared with that in the controls. CD82 mRNA expression levels in pediatric patients with B cell-ALL (B-ALL) were higher than those in ALL-CR patients and controls. For T-ALL, CD82 expression in ND patients was higher than in controls. CD133 mRNA expression levels in patients with pediatric B-ALL-ND were higher than that of controls and patients with ALL-CR. The frequency of CD34+ cells in pediatric ALL was significantly higher than that in controls. Frequencies of CD34+CD133+ or CD34+CD82+ cells in pediatric ALL were higher than those in controls. A positive association was observed between CD133 and CD82 mRNA expression in patients with B-ALL. A significant association was observed between CD133 mRNA expression and the hyperdiploid karyotype. Therefore, it was considered that CD133 and CD82 may serve an important role in the evolution of pediatric ALL. CD133 and CD82 should be considered as potential markers for the prognosis of patients with ALL.

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“…The formation of spheroidal derivations in the growth dynamics in culture is believed to be the fundamental property of brain tumour stem cells (BTSC), which determines the initiation and proliferation of tumours in brain tissue (Laks et al, 2009) and is similar to the NSC/NPCs property to form the "neurospheres". It should be noted that the similarity of characteristics between the glioma cells and NSCs: a similar phenotype, proteome, secretome, comparable profile of receptor expression, adhesion molecules and identical targets of migration (Nakano, 2015;Yamamuro et al, 2015;Song et al, 2016;Amodeo et al, 2017;Garcia et al, 2017;Mehta & Lo Cascio, 2017;Qin et al, 2017) -is assumed as phylogenetic and it is suggested that brain tumours are derived from NSC/NPCs undergoing aberrant changes, -BTSCs (Kusne & Sanai, 2015;Stangeland et al, 2015;Zong et al, 2015;Ledur et al, 2016;Okawa et al, 2017). The content of BTSCs in glioma tissue enlarges proportionally with an increase in their malignancy, which is accompanied by raised expression levels of immunocytochemical markers of their stem phenotype (Bao et al, 2014;Wang et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

In vitro effects of platelet-derived factors of brain glioma patients on C6 glioma cells

Любич

Lisyanyi

Малышева

et al. 2019

Regul. Mech. Biosyst.

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Platelets play an important part in the progression and pathological angiogenesis of brain glioma because of the different granules content and release of microvesicles that are the source of numerous mediators and bioactive substances, which probably provides a "strategy" for the tumour survival. The objective of study was exploring the effect of platelet-released secretion products of patients with brain glioma on the experimental model of tumour growth in vitro. For this purpose, the cells of glioma C6 were cultured for 72 hours under the addition of modified media containing platelet-released secretion products or conditioned media of peripheral blood cells of patients with glioma as well as persons of the comparison group without rough somatic pathology. In control glioma C6 cultures in standard conditions cell clusters were formed by the type of "spheroids", from which radial cell migration occurred, a tense cellular or reticular growth zone was formed, and tumour cells preserved their ability to mitotic division. Under the influence of platelet-released secretion products of patients with glioma, differently directed effects on cell mitotic activity and the number of cell clusters in glioma C6 cultures were detected depending on the degree of tumour malignancy: stimulating effect under the influence of platelet factors of patients with high-malignancy glioma (G4) and inhibitory effect – due to the influence of platelet factors of patients with differentiated glioma (G2). In contrast to the thrombocyte-released factors, the conditioned media of a common pool of peripheral blood cells of patients with G4 glioma suppressed the mitotic activity of tumour cells and did not affect the number of cell clusters. No changes in glioma C6 cultures were revealed after the influence of platelet-released secretion products of persons of the comparison group. The obtained data confirm the important role of platelets in the pathogenesis of brain glioma, pointing to the fundamental difference in the spectrum of biologically active molecules that are released by platelets of patients depending on the degree of tumour malignancy and are able to regulate the cell cycle and proliferative activity of the glioma tumour cells, which may have application as a diagnostic marker as well as predictive marker of response to antitumour therapy.

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Characterization of glioma stem-like cells from human glioblastomas

Cited by 23 publications

References 31 publications

Multivoxel magnetic resonance spectroscopy identifies enriched foci of cancer stem-like cells in high-grade gliomas

Multivoxel magnetic resonance spectroscopy identifies enriched foci of cancer stem-like cells in high-grade gliomas

Aberrant expression of CD133 and CD82 in patients with pediatric acute lymphoblastic leukemia and the clinical significance

In vitro effects of platelet-derived factors of brain glioma patients on C6 glioma cells

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