Characterization of Glioma-Cells Derived From Human Polyomavirus-Induced Brain-Tumors in Hamsters

Raj, GV; Gordon, Jennifer; Logan, Tj; Hall, Dj; Am, DeLuca; Giordano, Antonio; Khalili, Kamel

doi:10.3892/ijo.7.4.801

Cited by 19 publications

(17 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…U-87MG (ATCC HTB14), a human glioblastoma cell line, was grown in Dulbecco's modified Eagle's medium supplemented with 10% heatinactivated fetal bovine serum and antibiotics (penicillin-streptomycin, 100 g/ ml) and was maintained at 37°C in a humidified atmosphere with 7% CO 2 . HJC-15b cells (37) derived from hamster brain tumors (59) which were induced by JCV were grown in Dulbecco's modified Eagle's medium supplemented with 10% heat-inactivated fetal calf serum and antibiotics (penicillin-streptomycin, 100 g/ml).…”

Section: Methodsmentioning

confidence: 99%

Members of the AP-1 Family, c-Jun and c-Fos, Functionally Interact with JC Virus Early Regulatory Protein Large T Antigen

Kim

Woolridge

Biffi

et al. 2003

J Virol

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Members of the AP-1 Family, c-Jun and c-Fos, Functionally Interact with JC Virus Early Regulatory Protein Large T Antigen

Kim

Woolridge

Biffi

et al. 2003

J Virol

View full text Add to dashboard Cite

“…13,14 Both cell lines express the JCV-early gene products, large T and small t antigens, under the control of the JCVearly promoter. To investigate the possible role of SF2/ASF in regulating the expression of large T antigen, T7-tagged SF2/ASF was introduced into BsB8 and HJC-2 cells by transient transfection (Fig.…”

Section: Jcv Tumor Antigen Expression Is Suppressed By Sf2/asf In Virmentioning

confidence: 99%

Extinction of Tumor Antigen Expression by SF2/ASF in JCV-Transformed Cells

et al. 2011

View full text Add to dashboard Cite

The human neurotropic polyomavirus JC (JCV) induces a broad range of neural-origin tumors in experimental animals and has been repeatedly detected in several human cancers, most notably neural crest-origin tumors including medulloblastomas and glioblastomas. The oncogenic activity of JCV is attributed to the viral early gene products, large T and small t antigens, as evident by results from in vitro cell culture and in vivo animal studies. Recently, we have shown that alternative splicing factor, SF2/ASF, has the capacity to exert a negative effect on transcription and splicing of JCV genes in glial cells through direct association with a specific DNA motif within the viral promoter region. Here, we demonstrate that SF2/ASF suppresses large T antigen expression in JCV-transformed tumor cell lines, and the expression of SF2/ASF in such tumor cells thereby inhibits the transforming capacity of the viral tumor antigens. Moreover, down-regulation of SF2/ASF in viral-transformed tumor cell lines induces growth and proliferation of the tumor cells. Mapping analysis of the minimal peptide domain of SF2/ASF responsible for JCV promoter silencing and tumor suppressor activity suggests that amino acid residues 76 to 100 of SF2/ASF are functionally sufficient to suppress the growth of the tumor cells. These observations demonstrate a role for SF2/ASF in JCVmediated cellular transformation and provide a new avenue of research to pathogenic mechanisms of JCV-induced tumors.

show abstract

“…Newborn Syrian hamsters develop a broad range of tumors, including medulloblastomas, primitive neuroectodermal tumors, astrocytomas, glioblastoma multiforme, and peripheral neuroblastomas after brain inoculation with JCV (Walker et al, 1973;Varakis, 1978;Zu Rhein and Varakis, 1979). While no evidence for productive infection of the tumor cells by JCV has been observed, expression of the viral early, but not late, promoter in the tumor cells leads to the accumulation of large amounts of T-antigen (Raj et al, 1995). Similarly, injection of JCV into the brains of newborn rats induces undifferentiated neuroectodermal tumors in the cerebrum of 75% of the animals (Ohsumi et al, 1986).…”

Section: Jcv and Cancer: In Vitro And In Vivo Observationsmentioning

confidence: 99%