Characterization of GAVAA receptor-mediated 36chloride uptake in rat brain synaptoneurosomes

Luu, My Do; Morrow, A. Leslie; Paul, Steven M.; Schwartz, Rochelle D.

doi:10.1016/0024-3205(87)90207-4

Cited by 33 publications

(14 citation statements)

References 32 publications

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“…However, it has been reported that the effective doses of non-benzodiazepine anxiolytics, such as 5-HT 1A agonists and 5-HT 3 antagonists, were lower in the plus-maze test than in the conflict test (Hogg 1996). Several drugs such as benzodiazepines, barbiturates, neurosteroids, ethanol, some of the anticonvulsants and general anaesthetics interact with GABA A receptors in the process of eliciting their pharmacological effects (Gee et al 1987 ;Luu et al 1987 ;Morrow & Paul 1988 ;Mehta & Ticku 1999). In particular, the traditional benzodiazepines develop pharmacological effects through benzodiazepine receptors that are modulatory sites coupled to the GABA A -receptor chloride channel (Ketelaars et al 1988 ;Haefely 1990 ;Sannerud et al 1993).…”

Section: Discussionmentioning

confidence: 99%

Confirmation of the anxiolytic-like effect of dihydrohonokiol following behavioural and biochemical assessments

Maruyama

Kuribara

Kishi

et al. 2001

Journal of Pharmacy and Pharmacology

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Previous studies in this laboratory revealed that dihydrohonokiol-B (DHH-B; 3'-(2 propenyl)-5-propyl-(1,1'-biphenyl)-2,4'-diol), a partially reduced derivative of honokiol, was an effective anxiolytic-like agent in mice at an oral dose of 0.04 mg kg(-1), and at higher doses, when evaluated by the elevated plus-maze test. The aim of this study was to further confirm the anxiolytic-like effect of DHH-B using an additional behavioural procedure (Vogel's conflict test in mice) and a biochemical assessment (in-vitro determination of muscimol-stimulated 36Cl- uptake into mouse cortical synaptoneurosomes). As in earlier experiments, DHH-B (0.04-1 mg kg(-1), p.o.) was shown to prolong the time spent in the open-sided arms of the elevated plus-maze in a dose-dependent manner. Moreover, in the Vogel's conflict test, DHH-B (5 mg kg(-1), p.o.) significantly increased punished water intake. In tests with mouse cerebral cortical synaptoneurosomes, 10 and 30 microM of DHH-B significantly increased 36Cl- influx in the absence of muscimol. In the presence of 25 microM muscimol, the addition of 1 microM DHH-B led to significant enhancement of 36Cl- uptake, while 30 microM DHH-B was required to further stimulate the 36Cl- uptake induced by 250 microM muscimol. The results of these studies confirm that DHH-B is a potent anxiolytic-like agent and that GABA(A) receptor-gated Cl(-)-channel complex is involved in the anxiolytic-like efficacy of DHH-B.

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Section: Discussionmentioning

confidence: 99%

Confirmation of the anxiolytic-like effect of dihydrohonokiol following behavioural and biochemical assessments

Maruyama

Kuribara

Kishi

et al. 2001

Journal of Pharmacy and Pharmacology

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“…Synaptoneurosomes were prepared and 36 Cl Ϫ uptake was conducted as previously described for cortex, pons, and midbrain (Luu et al, 1987;Morrow et al, 1988). The synaptoneurosome pellet was re-suspended in 2.4 volumes ice cold assay buffer (20 mM Hepes, 118 mM NaCl, 4.7 mM KCl,1.2 mM MgSO 4 , 2.5 mM CaCl 2 , ph 7.4) for a final protein concentration of approximately 5 mg/mL.…”

Section: Chloride Flux Assaymentioning

confidence: 99%

Chronic Ethanol Consumption Differentially Alters GABA_A Receptor α1 and α4 Subunit Peptide Expression and GABA_A Receptor‐Mediated ³⁶Cl⁻ Uptake in Mesocorticolimbic Regions of Rat Brain

Papadeas

Grobin

Morrow

2001

Alcoholism Clin & Exp Res

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“…The resulting material after a very similar preparation has been described in detail and is considered to mainly be made up of sealed neuronal membranes, often arranged in a "snow-man"-like fashion ("synaptoneurosomes"), with a very limited number of other "cells" (Hollingsworth et al, 1985). The method has previously been applied also to the rat brain and has been extensively characterized (Schwartz et al, 1984(Schwartz et al, , 1985Luu et al, 1987).…”

Section: Preparation Of Synaptoneurosomesmentioning

confidence: 98%

“…S6derpalm et al, 1995) on brain monoamine biochemistry and the possible interrelationship of NA and 5-HT neurons in the effects obtained. We have also tried to elucidate whether yohimbine alters the function of the GABAa/BDZ receptor complex, as evaluated in a previously described in vitro system (Schwartz et al, 1984(Schwartz et al, , 1985Luu et al, 1987;SOderpalm et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

The yohimbine-induced anticonflict effect in the rat, part II. Neurochemical findings

Söderpalm

Ehrenström

Söderpalm

1995

J. Neural Transmission

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In a companion paper the alpha 2-adrenoceptor antagonist yohimbine was found to produce a dose-dependent anticonflict effect in a modified Vogel's conflict test. The behavioral data further indicated that noradrenergic and serotonergic neurons as well as the benzodiazepine (BDZ) receptor may be involved in the anticonflict effect of yohimbine. In the present study the effects on rat brain monoamine neurochemistry and GABAA/BDZ receptor function (36Cl-uptake in corticohippocampal synaptoneurosomes) of a maximally anticonflict producing dose of yohimbine (4.0 mg/kg, i.p.) were studied. The levels of rat brain catecholamines and indoleamines were measured ex vivo using high performance liquid chromatography with electrochemical detection (HPLC-ED). Yohimbine decreased noradrenaline levels both in the hippocampus and the hemispheres but instead increased DOPAC levels in these brain regions as well as in the limbic forebrain. Yohimbine also markedly enhanced DOPA accumulation in the hippocampus and the hemispheres after inhibition of 1-aromatic amino acid decarboxylase by means of NSD 1015, whereas in the limbic system only a modest increase was obtained. The yohimbine-induced effects on the catecholamine synthesis rate were largely abolished in animals severely depleted of NA by means of 6-hydroxy-dopamine (6-OH-DA) pretreatment. Yohimbine decreased both the 5-HIAA/5-HT quotient (an indicator of 5-HT turnover) and 5-HTP accumulation after NSD 1015 in the hemispheres, whereas in the hippocampus and the limbic system only 5-HTP accumulation was decreased. The yohimbine-induced effect on the indoleamine synthesis rate was not influenced by 6-OH-DA pretreatment, whereas this effect and that on the catecholamine synthesis rate were both abolished by reserpine pretreatment. Neither in vivo nor in vitro administration of yohimbine significantly altered baseline or GABA-induced accumulation of 36Cl- in corticohippocampal synaptoneurosomes. In conclusion, the present study provides neurochemical support for the suggestion that yohimbine may exert its anticonflict effect in a modified Vogel's conflict test by increasing and decreasing NA and 5-HT neurotransmission, respectively, whereas no evidence was obtained for a direct interaction of yohimbine with GABAA/BDZ receptor function.

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Characterization of GAVAA receptor-mediated 36chloride uptake in rat brain synaptoneurosomes

Cited by 33 publications

References 32 publications

Confirmation of the anxiolytic-like effect of dihydrohonokiol following behavioural and biochemical assessments

Confirmation of the anxiolytic-like effect of dihydrohonokiol following behavioural and biochemical assessments

Chronic Ethanol Consumption Differentially Alters GABA_A Receptor α1 and α4 Subunit Peptide Expression and GABA_A Receptor‐Mediated ³⁶Cl⁻ Uptake in Mesocorticolimbic Regions of Rat Brain

The yohimbine-induced anticonflict effect in the rat, part II. Neurochemical findings

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Characterization of GAVAA receptor-mediated 36chloride uptake in rat brain synaptoneurosomes

Cited by 33 publications

References 32 publications

Confirmation of the anxiolytic-like effect of dihydrohonokiol following behavioural and biochemical assessments

Confirmation of the anxiolytic-like effect of dihydrohonokiol following behavioural and biochemical assessments

Chronic Ethanol Consumption Differentially Alters GABAA Receptor α1 and α4 Subunit Peptide Expression and GABAA Receptor‐Mediated 36Cl− Uptake in Mesocorticolimbic Regions of Rat Brain

The yohimbine-induced anticonflict effect in the rat, part II. Neurochemical findings

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Chronic Ethanol Consumption Differentially Alters GABA_A Receptor α1 and α4 Subunit Peptide Expression and GABA_A Receptor‐Mediated ³⁶Cl⁻ Uptake in Mesocorticolimbic Regions of Rat Brain