Characterization of functional, safety, and gut survival related characteristics of Lactobacillus strains isolated from farmhouse goat's milk cheeses

Lerma, Leyre Lavilla; Pulido, Rubén Pérez; Martínez‐Bueno, Manuel; Maqueda, Mercedes; Valdivia, Eva

doi:10.1016/j.ijfoodmicro.2013.02.015

Cited by 66 publications

(45 citation statements)

References 56 publications

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“…have a generally recognized as safe (GRAS) and a qualified presumption of safety (QPS) status, it is important to check the safety of each specific strain intended for use in a probiotic food formulation (Lavilla-Lerma et al 2013). In addition, probiotic bacteria should survive during the food processing steps and throughout the product shelf-life, and it is also desirable that they can offer a contribution to the sensory attributes of the final product.…”

Section: Introductionmentioning

confidence: 99%

Artisanal Coalho cheeses as source of beneficial Lactobacillus plantarum and Lactobacillus rhamnosus strains

Santos

Vieira

Buriti

et al. 2014

Dairy Sci. & Technol.

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Based on screening for potential beneficial lactic acid bacteria from Coalho cheese produced in the North-East region of Brazil, eight strains belonging to Lactobacillus rhamnosus and Lactobacillus plantarum were selected. All investigated strains presented low levels of hydrophobicity. Different levels of coaggregation were Dairy Sci. & Technol. (2015) 95:209-230 DOI 10.1007 observed for tested Lb. rhamnosus and Lb. plantarum with Listeria monocytogenes. All strains were able to grow in presence of 0.5% of the sodium salts of taurocholic acid (TC), taurodeoxycholic acid (TDC), and glycocholic acid (GC) and showed the ability to deconjugate only TC, TDC, and GC. Investigated Lb. rhamnosus and Lb. plantarum strains showed good survival when exposed to the conditions simulating the GIT conditions. Lb. rhamnosus and Lb. plantarum strains were tested for presence of virulence, antibiotic resistance, and biogenic amine production genes. In addition, minimum inhibititory concentration (MIC) of selected antibiotics was determined. Production of antimicrobial peptides (bacteriocins) was investigated. However, only Lb. rhamnosus EM253 produced bacteriocin at level 800 arbitrary unit (AU) mL −1 against L. monocytogenes 211. The bacteriocin remained stable at pH from 2.0 to 10.0 and after exposure at 100°C for 120 min and in presence of surfactants and salts. Studied Lb. rhamnosus and Lb. plantarum strains showed good potential to be applied as a functional coculture/s with beneficial properties in the production of Coalho cheese.

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Section: Introductionmentioning

confidence: 99%

Artisanal Coalho cheeses as source of beneficial Lactobacillus plantarum and Lactobacillus rhamnosus strains

Santos

Vieira

Buriti

et al. 2014

Dairy Sci. & Technol.

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“…In particular, CIP has a broad antibacterial spectrum, with bactericidal activity toward Gram-positive and Gram-negative aerobes (5,19); it accumulates inside the PMNs and remains active, leading to a significant reduction in the number of intracellular bacteria, such as Aggregatibacter actinomycetemcomitans (formerly Actinobacillus actinomycetemcomitans) (20).…”

Section: Discussionmentioning

confidence: 99%

“…From the literature, lactobacilli seem to be intrinsically resistant to fluoroquinolones, and resistance to CIP appears to be a natural trait in many species of Lactobacillus, such as L. plantarum (5,19).…”

Section: Discussionmentioning

confidence: 99%

Key Roles of Human Polymorphonuclear Cells and Ciprofloxacin in Lactobacillus Species Infection Control

Mandras

Tullio

Furneri

et al. 2016

Antimicrob Agents Chemother

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Lactobacilli have the potential to act as reservoirs of antibiotic resistance genes similar to those found in human pathogens, with the risk of transferring these genes to many pathogenic bacteria. In this study, we investigated the role of human polymorphonuclear cells (PMNs) against Lactobacillus spp. both resistant and susceptible to ciprofloxacin (a fluoroquinolone) and the effect of ciprofloxacin on the interaction between PMNs and three Lactobacillus spp. with different patterns of susceptibility to this drug. Hence, the primary functions of PMNs, such as phagocytosis and bacterial intracellular killing, against lactobacilli were investigated. The rate of PMN phagocytosis was high for ciprofloxacin-sensitive and ciprofloxacin-resistant strains. The patterns of intracellular killing of ciprofloxacin-sensitive and ciprofloxacin-resistant strains by PMNs underline that PMNs alone were able to kill lactobacilli. The addition of ciprofloxacin to PMNs did not result in a significant increase in the bacterial uptake by phagocytes. On the contrary, ciprofloxacin had a marked effect on PMN intracellular killing, resulting in increased numbers of killed ciprofloxacin-sensitive bacteria in comparison with antibiotic-free controls. Our data show that by itself, the profile of antibiotic resistance does not constitute an intrinsic factor of greater or lesser pathogenicity toward the host. The ability of PMNs to kill a diverse array of bacterial pathogens is essential for human innate host defense, primarily in immunocompromised patients. Lactobacillus spp., considered nonpathogenic bacteria of the human gastrointestinal and vaginal microbiotas, are widely used as probiotics in dairy products. Moreover, they can positively interact with other microorganisms of the human microbiota (1).Infections caused by lactobacilli are extremely rare; however, they can spread into the blood and cause serious clinical infections (2, 3). Many investigators have demonstrated that lactobacilli have a high natural resistance to many antimicrobial drugs, such as fluoroquinolones, e.g., ciprofloxacin (CIP) (4-7); our recent studies showed that the mechanism of resistance to fluoroquinolones could be associated with the presence of a NorA-like efflux pump found in ciprofloxacin-resistant (CIP r ) strains of L. fermentum isolated from the human oral cavity (8).For several decades, studies on selection and dissemination of antibiotic resistance have focused mainly on clinically relevant species. The magnitude of the problem is significantly increased by the ability of bacteria to transfer resistance horizontally and by the mounting increase in the overuse and misuse of antibiotics, which has created an enormous selective pressure toward resistant bacteria.Gene transfer occurs widely in vivo between pathogenic and commensal bacteria of the human microbiota, as identical resistance genes are present in several bacterial species from different hosts. Lactobacilli have the potential to act as reservoirs of antibiotic resistance genes similar to th...

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“…Adhesion to intestinal mucus is a wanted attribute for probiotic bacteria, as it increases persistence in the GIT (Gastrointestinal Tract) and the aptitude to colonize intestine efficiently [16,17]. In vitro assay facilitates a preliminary selection of strains with probiotic potential, focusing on significant features as adhesion and safety properties, antibiotics susceptibility.…”

Section: Introductionmentioning

confidence: 99%

Safety and Antioxidant Properties of Five Probiotic <i>Lactobacillus plantarum</i> Strains Isolated from the Digestive Tract of Honey Bees

Kenfack¹,

Ngoufack²,

Kaktcham³

et al. 2018

AJMR

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The objective of this study was the evaluation of safety, adhesion and antioxidant properties of five L. plantarum strains isolated from the digestive tract of honey bees. The set of variables generated from this study was submitted to a normalized Pearson (n) Principal Component Analysis (PCA). The five L. plantarum strains showed no gelatinase activity and were checked to be non-hemolytic. They were susceptible to chloramphenicol, amoxicillin, penicillin G and tetracycline. The MICs were ranged between 1-4 µg/ml for erythromycin and resistance was observed among 80% of strains (L. plantarum H15, H21, H24, and H28). A significantly high percentage of hydrophobicity in n-hexane was observed with L. plantarum H47 (71.99±1.39) followed by L. plantarum H28 (65.68±1.49) while the highest value in the presence of chloroform, belonging L. plantarum H24 and L. plantarum H28 (28.39±0.88 and 23.58±0.68 respectively). Both the Intact Cells (ICs) and Cell-Free Supernatants (CFSs) of L. plantarum H24 strain displayed the higher percentage (p 0.05) of DPPH radical scavenging activity (76.58±0.55% and 59.13±4.01% respectively). With the HRS activity, ICs of L. plantarum H24 strain exhibited the highest (p > 0.05) activity (73.37±0.62%) whereas, the CFS of L. plantarum H47 was the best (29.49±1.28%, p > 0.05). In allpurpose, based on PCA, L. plantarum H28, and L. plantarum H24 seem to be quite promising as they possessed the best properties tested. Accordingly, they can be chosen as representative of the potential probiotic strains.

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Characterization of functional, safety, and gut survival related characteristics of Lactobacillus strains isolated from farmhouse goat's milk cheeses

Cited by 66 publications

References 56 publications

Artisanal Coalho cheeses as source of beneficial Lactobacillus plantarum and Lactobacillus rhamnosus strains

Artisanal Coalho cheeses as source of beneficial Lactobacillus plantarum and Lactobacillus rhamnosus strains

Key Roles of Human Polymorphonuclear Cells and Ciprofloxacin in Lactobacillus Species Infection Control

Safety and Antioxidant Properties of Five Probiotic <i>Lactobacillus plantarum</i> Strains Isolated from the Digestive Tract of Honey Bees

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