Characterization of full-length transcriptome and mechanisms of sugar accumulation in Annona squamosa fruit

Ren, Fang; Huang, Weixiong; Yao, Jinai; Xing, Liu; Zhang, Ji; Zhou, Shuangyun; Deng, Bingbing; ZhenYu, An

doi:10.32604/biocell.2020.012933

Cited by 7 publications

(2 citation statements)

References 45 publications

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“…The investigation conducted by Chel-Guerrero et al [7] concurs with the absence of sucrose in sugar apple peel. We can also note the discoveries of Pal & Kumar [30] revealing that sugar apple fruit contains 15% of total sugar content during the commercial maturity stage, while Fang et al [31] report that during fruit ripening there is an accumulation of sugars, mainly glucose and fructose, which contributes to the sweetness of the fruit. Furthermore, genes involved with starch degradation are up-regulated while genes related to starch synthesis are highly repressed during late development and ripening stages.…”

Section: Quantitative Analysismentioning

confidence: 56%

Chemical Profile and Study of the Antidiabetic Effect of Annona squamosa L. Peel

Ponce-Sánchez

Oidor-Chan²,

Álvarez-Ramírez

et al. 2023

Waste Biomass Valor

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Type 2 Diabetes Mellitus (T2DM) is considered a public health problem worldwide. Current treatment involves the use of oral antidiabetics, whereas natural products could be a therapeutic alternative because of their bioactive compounds. The objective of this research was to investigate the chemical pro le and antidiabetic effect of the peel of Annona squamosa L. (sugar apple) and evaluate a possible mechanism of action. We analyzed the content of secondary metabolites nding the following in 100 g of freeze-dried peel: 5000.

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Section: Quantitative Analysismentioning

confidence: 56%

Chemical Profile and Study of the Antidiabetic Effect of Annona squamosa L. Peel

Ponce-Sánchez

Oidor-Chan²,

Álvarez-Ramírez

et al. 2023

Waste Biomass Valor

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“…This approach can provide accurate information of transcript structure and alternative splicing. In fruit crops, this sequencing technology has been used to identify potential genes related to disease resistance in apple [14], proanthocyanidin accumulation in persimmon [15], carotenoid biosynthesis in avocado [16], and sugar-metabolism in Annona squamosa [17]. In this study, we developed the first full-length fruit transcriptome for two blueberry cultivars, a tetraploid southern highbush 'Suziblue' and hexaploid cultivar 'Powderblue' .…”

Section: Introductionmentioning

confidence: 99%

Full-length fruit transcriptomes of southern highbush (Vaccinium sp.) and rabbiteye (V. virgatum Ait.) blueberry

Wang

Nambeesan

2022

BMC Genomics

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Background Blueberries (Vaccinium sp.) are native to North America and breeding efforts to improve blueberry fruit quality are focused on improving traits such as increased firmness, enhanced flavor and greater shelf-life. Such efforts require additional genomic resources, especially in southern highbush and rabbiteye blueberries. Results We generated the first full-length fruit transcriptome for the southern highbush and rabbiteye blueberry using the cultivars, Suziblue and Powderblue, respectively. The transcriptome was generated using the Pacific Biosciences single-molecule long-read isoform sequencing platform with cDNA pooled from seven stages during fruit development and postharvest storage. Raw reads were processed through the Isoseq pipeline and full-length transcripts were mapped to the ‘Draper’ genome with unmapped reads collapsed using Cogent. Finally, we identified 16,299 and 15,882 non-redundant transcripts in ‘Suziblue’ and ‘Powderblue’ respectively by combining the reads mapped to Northern Highbush blueberry ‘Draper’ genome and Cogent analysis. In both cultivars, > 80% of sequences were longer than 1,000 nt, with the median transcript length around 1,700 nt. Functionally annotated transcripts using Blast2GO were > 92% in both ‘Suziblue’ and ‘Powderblue’ with overall equal distribution of gene ontology (GO) terms in the two cultivars. Analyses of alternative splicing events indicated that around 40% non-redundant sequences exhibited more than one isoform. Additionally, long non-coding RNAs were predicted to represent 5.6% and 7% of the transcriptomes in ‘Suziblue’ and ‘Powderblue’, respectively. Fruit ripening is regulated by several hormone-related genes and transcription factors. Among transcripts associated with phytohormone metabolism/signaling, the highest number of transcripts were related to abscisic acid (ABA) and auxin metabolism followed by those for brassinosteroid, jasmonic acid and ethylene metabolism. Among transcription factor-associated transcripts, those belonging to ripening-related APETALA2/ethylene-responsive element-binding factor (AP2/ERF), NAC (NAM, ATAF1/2 and CUC2), leucine zipper (HB-zip), basic helix-loop-helix (bHLH), MYB (v-MYB, discovered in avian myeloblastosis virus genome) and MADS-Box gene families, were abundant. Further we measured three fruit ripening quality traits and indicators [ABA, and anthocyanin concentration, and texture] during fruit development and ripening. ABA concentration increased during the initial stages of fruit ripening and then declined at the Ripe stage, whereas anthocyanin content increased during the final stages of fruit ripening in both cultivars. Fruit firmness declined during ripening in ‘Powderblue’. Genes associated with the above parameters were identified using the full-length transcriptome. Transcript abundance patterns of these genes were consistent with changes in the fruit ripening and quality-related characteristics. Conclusions A full-length, well-annotated fruit transcriptome was generated for two blueberry species commonly cultivated in the southeastern United States. The robustness of the transcriptome was verified by the identification and expression analyses of multiple fruit ripening and quality–regulating genes. The full-length transcriptome is a valuable addition to the blueberry genomic resources and will aid in further improving the annotation. It will also provide a useful resource for the investigation of molecular aspects of ripening and postharvest processes.

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Multi-omic applications for understanding and enhancing tropical fruit flavour

Lomax,

Ford,

Bar

2024

Plant Mol Biol

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Consumer trends towards nutrient-rich foods are contributing to global increasing demand for tropical fruit. However, commercial cultivars in the breeding pipeline that are tailored to meet market demand are at risk of possessing reduced fruit flavour qualities. This stems from recurrent prioritised selection for superior agronomic traits and not fruit flavour, which may in turn reduce consumer satisfaction. There is realisation that fruit quality traits, inclusive of flavour, must be equally selected for; but currently, there are limited tools and resources available to select for fruit flavour traits, particularly in tropical fruit species. Although sugars, acids, and volatile organic compounds are known to define fruit flavour, the specific combinations of these, that result in defined consumer preferences, remain unknown for many tropical fruit species. To define and include fruit flavour preferences in selective breeding, it is vital to determine the metabolites that underpin them. Then, objective quantitative analysis may be implemented instead of solely relying on human sensory panels. This may lead to the development of selective genetic markers through integrated omics approaches that target biosynthetic pathways of flavour active compounds. In this review, we explore progress in the development of tools to be able to strategically define and select for consumer-preferred flavour profiles in the breeding of new cultivars of tropical fruit species.

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Characterization of full-length transcriptome and mechanisms of sugar accumulation in Annona squamosa fruit

Cited by 7 publications

References 45 publications

Chemical Profile and Study of the Antidiabetic Effect of Annona squamosa L. Peel

Chemical Profile and Study of the Antidiabetic Effect of Annona squamosa L. Peel

Full-length fruit transcriptomes of southern highbush (Vaccinium sp.) and rabbiteye (V. virgatum Ait.) blueberry

Multi-omic applications for understanding and enhancing tropical fruit flavour

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