Characterization of four melanoma cell lines with electron microscopy, immunocytochemistry, cytogenetics, flow cytometry, and southern analysis

Köpf, I; Stierner, Ulrika; Islam, Quamrul; Delle, Ulla; Kindblom, Lars‐Gunnar; Martinsson, Tommy

doi:10.1016/0165-4608(92)90248-7

Cited by 9 publications

(3 citation statements)

References 41 publications

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“…It is of interest to note that the cultured tumor cells had preserved the immunohistochemical characteristics and some of the ultrastructural features of the primary tumor, supporting the representativity of the cultures used for the cytogenetic analysis. Retained ultrastructural and immunohistochemical melanoma characteristics have previously been observed in cell lines of malignant melanoma (Kopf et al, 1991) and in a cell line established from an amelanotic clear-cell sarcoma (Epstein et al, 1984). Moreover, the in vitro growth characteristics of the present tumor, with a predominance of stellate and dendritic tumor cells and large scattered, polymorphic, cytoplasm-rich cells, resemble those in recent studies of established malignant melanoma cell lines (Kopf et al, 1991).…”

Section: Discussionmentioning

confidence: 90%

Reciprocal translocation t(12;22)(q13;q13) in clearcell sarcoma of tendons and aponeuroses

Stenman

Kindblom

Angervall

1992

Genes Chromosomes & Cancer

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Clear-cell sarcoma is a rare soft tissue sarcoma that displays certain similarities to malignant melanoma. In this paper we describe the karyotypic findings and in vitro growth characteristics of a short-term-cultured clear-cell sarcoma. The cultured tumor cells had preserved immunohistochemical characteristics and certain ultrastructural features of the primary tumour, including positivity for vimentin, S-100 protein, and a melanoma-associated antigen, supporting the authenticity of the cultured cells. Cytogenetic analysis revealed an abnormal stemline karyotype of 49,XY, -1, +8, +8, +12, +der(1)t(1;?)(p36.1-.3;?), t(12;22)(q13;q13). A similar or identical t(12;22) was recently reported in two of four clear-cell sarcomas. It is suggested that the t(12;22)(q13;q13) is a primary cytogenetic abnormality in clear-cell sarcoma and distinguishes this tumor type from malignant melanoma.

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Section: Discussionmentioning

confidence: 90%

Reciprocal translocation t(12;22)(q13;q13) in clearcell sarcoma of tendons and aponeuroses

Stenman

Kindblom

Angervall

1992

Genes Chromosomes & Cancer

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“…This locus has a long history in melanoma that started with detection of isochromosome 9q with cytogenetic analysis. 6,7 Loss of heterozygosity (LOH) of markers at 9q22 was subsequently frequently reported and was shown to be associated with proliferation and tumor progression. 8,9 Recently, single nucleotide polymorphism (SNP) analysis has confirmed the LOH of this locus in melanoma, while genome-wide analysis in dizygotic twins for nevi numbers also showed linkage with this 9q region.…”

mentioning

confidence: 99%

Epigenetic Regulation IdentifiesRASEFas a Tumor-Suppressor Gene in Uveal Melanoma

Maat¹,

Beiboer

Jager³

et al. 2008

Invest. Ophthalmol. Vis. Sci.

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“…The cultures were kept at 37°C in a water-saturated atmosphere containing 5% COz. Light and electron microscopic and immunocytochemical examinations of the cultured cells (case 1) were performed as previously described (Kopf et al, 1992;Stenman et al, 1992).…”

Section: Cell Culture and Cytogenetic Analysismentioning

confidence: 99%

Translocation t(9;22)(q22;q12) is a primary cytogenetic abnormality in extraskeletal myxoid chondrosarcoma

Stenman

Andersson

Mandahl

et al. 1995

Intl Journal of Cancer

104

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The cytogenetic and in vitro growth characteristics of 3 cases of extraskeletal myxoid chondrosarcoma (EMC) are described. In cell culture, the tumor cells retained the immunocytochemical and ultrastructural characteristics of EMC. Cytogenetically, 2 of the cases showed an apparently identical t(9;22)(q22;q12). In one case, the t(9;22) was found together with a dup(I)(q12q44), and in the other case it was found together with several other aberrations. The third case had an inv(10)(p11.2q22) as the sole karyotypic abnormality. Of 4 cases of EMC previously analyzed, 2 showed a t(9;22)(q22;q11-12) and one case of a t(9;22;15)(q31;q12.2;q25). Thus, 5 out of 7 cases of EMC showed recombination between 9q22-31 and 22q11-12, indicating that this represents a tumor specific abnormality. The breakpoints on 22q were in all 5 cases cytogenetically indistinguishable from those seen in Ewing's sarcoma with t(11;22), clear-cell sarcoma with t(12;22), and desmoplastic small round cell tumors with t(11;22). Molecular cloning of these translocation breakpoints revealed involvement of the EWS gene (located at 22q12) in all 3 tumor types. These observations raise the intriguing question of whether the EWS gene might also be involved in the t(9;22) in EMC.

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Characterization of four melanoma cell lines with electron microscopy, immunocytochemistry, cytogenetics, flow cytometry, and southern analysis

Cited by 9 publications

References 41 publications

Reciprocal translocation t(12;22)(q13;q13) in clearcell sarcoma of tendons and aponeuroses

Reciprocal translocation t(12;22)(q13;q13) in clearcell sarcoma of tendons and aponeuroses

Epigenetic Regulation IdentifiesRASEFas a Tumor-Suppressor Gene in Uveal Melanoma

Translocation t(9;22)(q22;q12) is a primary cytogenetic abnormality in extraskeletal myxoid chondrosarcoma

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