Characterization of Fortilin, a Novel Antiapoptotic Protein

Li, Franklin; Zhang, Di; Fujise, Ken

doi:10.1074/jbc.m108954200

Cited by 255 publications

(275 citation statements)

References 56 publications

Supporting

Mentioning

266

Contrasting

Order By: Relevance

“…Another milestone in the unravelling of TCTP's function was the demonstration of its anti-apoptotic activity by Ken Fujise's group (Houston) (Li et al 2001), exactly 20 years after its initial discovery (Thomas et al 1981;Yenofsky et al 1982). Based on their findings, these authors invented the name 'fortilin' for this protein.…”

Section: The 'Translationally Controlled Tumour Protein Tctp': Names mentioning

confidence: 99%

“…The anti-apoptotic activity of TCTP was originally discovered in Ken Fujise's laboratory, where the name 'fortilin' was coined (Li et al 2001). Their study described the cytoprotective effect of TCTP/fortilin overexpression against etoposide-induced apoptosis in HeLa cells.…”

Section: Anti-apoptotic Activity: Discovery and Mechanismsmentioning

confidence: 99%

“…It is therefore not surprising that TCTP is often found to be overexpressed in cancer, alongside other 'classical' anti-apoptotic proteins, such as Bcl-2, Bcl-XL or Mcl-1. Since the discovery of the anti-apoptotic activity of TCTP (fortilin) (Li et al 2001), several mechanistic aspects of this property have been reported (reviewed in Bommer 2012; Acunzo et al 2014;Chan et al 2012a). These were described earlier in Sect.…”

Section: Mechanistic Involvement In Cancer Progressionmentioning

confidence: 99%

“…the antagonism of TCTP to the tumour suppressor protein p53, which is crucial for TCTP to exert its cancer-promoting activity (Amson et al 2012;Chen et al 2011). The cytoprotective role of TCTP in cancer cells is well documented, for example in conditions of oxidative stress (Lucibello et al 2011), or of treatment with various anticancer drugs, the latter often resulting in chemoresistance of these cells (Li et al 2001;Jung et al 2014;Graidist et al 2004;Bommer et al 2017;He et al 2015), which is a frequent problem in cancer chemotherapy. 2.…”

Section: Mechanistic Involvement In Cancer Progressionmentioning

confidence: 99%

“…They identified TCTP as one of four proteins overexpressed in those cell lines, compared to their no-resistant parental cells (Sinha et al 2000). Fujise's laboratory demonstrated TCTP (fortilin)-dependent drug resistance against etoposide (Li et al 2001) and 5-FU in U2OS cells. In a more recent study, Kyunglim Lee's laboratory explored the mechanism underlying the TCTP-dependent resistance against etoposide in HeLa cells.…”

Section: Mechanistic Involvement In Cancer Progressionmentioning

confidence: 99%

See 4 more Smart Citations

The Translational Controlled Tumour Protein TCTP: Biological Functions and Regulation

Bommer

2017

Results and Problems in Cell Differentiation

View full text Add to dashboard Cite

The Translational Controlled Tumour Protein TCTP (gene symbol TPT1, also called P21, P23, Q23, fortilin or histamine-releasing factor, HRF) is a highly conserved protein present in essentially all eukaryotic organisms and involved in many fundamental cell biological and disease processes. It was first discovered about 35 years ago, and it took an extended period of time for its multiple functions to be revealed, and even today we do not yet fully understand all the details. Having witnessed most of this history, in this chapter, I give a brief overview and review the current knowledge on the structure, biological functions, disease involvements and cellular regulation of this protein. TCTP is able to interact with a large number of other proteins and is therefore involved in many core cell biological processes, predominantly in the response to cellular stresses, such as oxidative stress, heat shock, genotoxic stress, imbalance of ion metabolism as well as other conditions. Mechanistically, TCTP acts as an anti-apoptotic protein, and it is involved in DNA-damage repair and in cellular autophagy. Thus, broadly speaking, TCTP can be considered a cytoprotective protein. In addition, TCTP facilitates cell division through stabilising the mitotic spindle and cell growth through modulating growth signalling pathways and through its interaction with the proteosynthetic machinery of the cell. Due to its activities, both as an anti-apoptotic protein and in promoting cell growth and division, TCTP is also essential in the early development of both animals and plants. Apart from its involvement in various biological processes at the cellular level, TCTP can also act as an extracellular protein and as such has been involved in modulating whole-body defence processes, namely in the mammalian immune system. Extracellular TCTP, typically in its dimerised form, is able to induce the release of cytokines and other signalling molecules from various types of immune cells. There are also several examples, where TCTP was shown to be involved in antiviral/antibacterial defence in lower animals. In plants, the protein appears to have a protective effect against phytotoxic stresses, such as flooding, draught, too high or low temperature, salt stress or exposure to heavy metals. The finding for the latter stress condition is corroborated by earlier reports that TCTP levels are considerably up-regulated upon exposure of earthworms to high levels of heavy metals. Given the involvement of TCTP in many biological processes aimed at maintaining cellular or whole-body homeostasis, it is not surprising that dysregulation of TCTP levels may promote a range of disease processes, foremost cancer. Indeed a large body of evidence now supports a role of TCTP in at least the most predominant types of human cancers. Typically, this can be ascribed to both the anti-apoptotic activity of the protein and to its function in promoting cell growth and division. However, TCTP also appears to be involved in the later stages of cancer progression, such ...

show abstract

Section: The 'Translationally Controlled Tumour Protein Tctp': Names mentioning

confidence: 99%

Section: Anti-apoptotic Activity: Discovery and Mechanismsmentioning

confidence: 99%

Section: Mechanistic Involvement In Cancer Progressionmentioning

confidence: 99%

Section: Mechanistic Involvement In Cancer Progressionmentioning

confidence: 99%

Section: Mechanistic Involvement In Cancer Progressionmentioning

confidence: 99%

See 3 more Smart Citations

The Translational Controlled Tumour Protein TCTP: Biological Functions and Regulation

Bommer

2017

Results and Problems in Cell Differentiation

View full text Add to dashboard Cite

show abstract

siRNA targeting TCTP suppresses osteosarcoma cell growth and induces apoptosis in vitro and in vivo

Shen

Chu

et al. 2015

Biotech and App Biochem

View full text Add to dashboard Cite

Osteosarcoma (OS) remains the most frequent primary malignant bone tumor in adolescents. However, the molecular cause of the disease is poorly elucidated. In the present study, we primarily found that translationally controlled tumor protein (TCTP) was overexpressed in human OS tissues and cell lines. To investigate the function of TCTP in OS cell growth, an RNA interference lentivirus system was employed to deplete TCTP expression in Saos-2 and U2OS cell lines. Specific knockdown of TCTP significantly impaired cell proliferation and colony-formation capacity in both OS cell lines. Moreover, depletion of TCTP caused a significant accumulation of OS cells in the S phase and eventually induced cell apoptosis. Expression levels of the G2/M phase regulators cyclin B1 and Cdc25A were decreased, and apoptotic markers Bad and caspase-3 were increased in both OS cell lines after depletion of TCTP. Furthermore, depletion of TCTP potently inhibited the growth of xenografts in nude mice. Our results indicate that inhibition of TCTP expression exerts potential antitumor activity and may be a novel therapeutic approach in human OS.

show abstract

‘Omics driven discoveries of gene targets for apoptosis attenuation in CHO cells

Orellana

Martínez

MacDonald

et al. 2020

Biotech & Bioengineering

View full text Add to dashboard Cite

Chinese hamster ovary (CHO) cells are widely used in biopharmaceutical production. Improvements to cell lines and bioprocesses are constantly being explored. One of the major limitations of CHO cell culture is that the cells undergo apoptosis, leading to rapid cell death, which impedes reaching high recombinant protein titres. While several genetic engineering strategies have been successfully employed to reduce apoptosis, there is still room to further enhance CHO cell lines performance. 'Omics analysis is a powerful tool to better understand different phenotypes and for the identification of gene targets for engineering. Here, we present a comprehensive review of previous CHO 'omics studies that revealed changes in the expression of apoptosis-related genes. We highlight targets for genetic engineering that have reduced, or have the potential to reduce, apoptosis or to increase cell proliferation in CHO cells, with the final aim of increasing productivity.

show abstract

Characterization of Fortilin, a Novel Antiapoptotic Protein

Cited by 255 publications

References 56 publications

The Translational Controlled Tumour Protein TCTP: Biological Functions and Regulation

The Translational Controlled Tumour Protein TCTP: Biological Functions and Regulation

siRNA targeting TCTP suppresses osteosarcoma cell growth and induces apoptosis in vitro and in vivo

‘Omics driven discoveries of gene targets for apoptosis attenuation in CHO cells

Contact Info

Product

Resources

About