Characterization of Forskolin-Induced Ca2+ Signals in Rat Olfactory Receptor Neurons

Otsuguro, Ken-ichi; Gautam, Shree Hari; S, Itó; Habara, Yoshiaki

doi:10.1254/jphs.fp0040883

Cited by 16 publications

(11 citation statements)

References 35 publications

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“…The OSNs displayed robust increases in somatic Ca 2' when stimulated by forskolin in ways different from that of acutely dissociated OSNs. The time taken to reach the peak level in our study was 65.4912.2 s, which is slower than the results of Otsuguro et al [20], although calculated by different methods. Also, the overall response rate of 82% is larger than that of isolated OSNs prepared from OE with papain digest [20].…”

Section: Discussioncontrasting

confidence: 85%

“…The time taken to reach the peak level in our study was 65.4912.2 s, which is slower than the results of Otsuguro et al [20], although calculated by different methods. Also, the overall response rate of 82% is larger than that of isolated OSNs prepared from OE with papain digest [20]. The discrepancy between our results and others needs further study to explain whether it was caused by the different culture methods.…”

Section: Discussioncontrasting

confidence: 85%

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Neurons growing from neonatal murine olfactory explant culture responded to forskolin

Zhang

Xian

Fan

et al. 2009

Acta Oto-Laryngologica

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Section: Discussioncontrasting

confidence: 85%

Section: Discussioncontrasting

confidence: 85%

Neurons growing from neonatal murine olfactory explant culture responded to forskolin

Zhang

Xian

Fan

et al. 2009

Acta Oto-Laryngologica

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“…In this work, OSN were perfused with forskolin, a specific adenylate cyclase stimulator. A plausible explanation of our results is that the forskolin-induced increase in intracellular free Ca 2+ was achieved by the activation of the olfactory transduction pathway, which resulted in downstream opening of VACC, as has been observed elsewhere in OSN obtained from other mammals [ 49 , 50 ]. On the other hand, the subtle forskolin-induced response observed in precursor cells could be related with the opening of nucleotide-operated Ca 2+ channels or with another transiently expressed mechanism; however, further research is required to elucidate this hypothesis.…”

Section: Discussionsupporting

confidence: 72%

Voltage-Activated Calcium Channels as Functional Markers of Mature Neurons in Human Olfactory Neuroepithelial Cells: Implications for the Study of Neurodevelopment in Neuropsychiatric Disorders

Solís‐Chagoyán

Flores‐Soto

Valdés-Tovar

et al. 2016

IJMS

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In adulthood, differentiation of precursor cells into neurons continues in several brain structures as well as in the olfactory neuroepithelium. Isolated precursors allow the study of the neurodevelopmental process in vitro. The aim of this work was to determine whether the expression of functional Voltage-Activated Ca2+ Channels (VACC) is dependent on the neurodevelopmental stage in neuronal cells obtained from the human olfactory epithelium of a single healthy donor. The presence of channel-forming proteins in Olfactory Sensory Neurons (OSN) was demonstrated by immunofluorescent labeling, and VACC functioning was assessed by microfluorometry and the patch-clamp technique. VACC were immunodetected only in OSN. Mature neurons responded to forskolin with a five-fold increase in Ca2+. By contrast, in precursor cells, a subtle response was observed. The involvement of VACC in the precursors’ response was discarded for the absence of transmembrane inward Ca2+ movement evoked by step depolarizations. Data suggest differential expression of VACC in neuronal cells depending on their developmental stage and also that the expression of these channels is acquired by OSN during maturation, to enable specialized functions such as ion movement triggered by membrane depolarization. The results support that VACC in OSN could be considered as a functional marker to study neurodevelopment.

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“…The contribution of the Ca 2ϩ release from stores in OSN signaling is still controversial, and different results have been obtained in different preparations Otsuguro et al, 2005). Here we found that cAMP, produced upon forskolin or odor administration in Ca 2ϩ -free medium, can induce an increase in cytosolic Ca 2ϩ .…”

Section: Discussionmentioning

confidence: 56%

Interplay among cGMP, cAMP, and Ca2+ in Living Olfactory Sensory Neurons In Vitro and In Vivo

Pietrobon¹,

Zamparo²,

Maritan³

et al. 2011

Journal of Neuroscience

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ThemechanismofcGMPproductioninolfactorysensoryneurons(OSNs)ispoorlyunderstood,althoughthismessengertakespartinseveralkey processes such as adaptation, neuronal development, and long-term cellular responses to odorant stimulation. Many aspects of the regulation of cGMP in OSNs are still unknown or highly controversial, such as its subcellular heterogeneity, mechanism of coupling to odorant receptors and downstream targets. Here, we have investigated the dynamics and the intracellular distribution of cGMP in living rat OSNs in culture transfected with a genetically encoded sensor for cGMP. We demonstrate that OSNs treated with pharmacological stimuli able to activate membrane or soluble guanylyl cyclase (sGC) presented an increase in cGMP in the entire neuron, from cilia-dendrite to the axon terminus-growth cone. Upon odorant stimulation, a rise in cGMP was again found in the entire neuron, including the axon terminus, where it is locally synthesized. The odorant-dependent rise in cGMP is due to sGC activation by nitric oxide (NO) and requires an increase of cAMP. The link between cAMP and NO synthase appears to be the rise in cytosolic Ca 2ϩ concentration elicited by either plasma membrane Ca 2ϩ channel activation or Ca 2ϩ mobilization from stores via the guanine nucleotide exchange factor Epac. Finally, we show that a cGMP rise can elicit both in vitro and in vivo the phosphorylation of nuclear CREB, suggesting that this signaling pathway may be relevant for both local events (pathfinding, neurotransmitter release) and more distal processes involving gene expression regulation.

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Characterization of Forskolin-Induced Ca2+ Signals in Rat Olfactory Receptor Neurons

Abstract: Abstract. Forskolin-induced Ca2+ signals were examined in isolated rat olfactory receptor neurons (ORNs) using a Ca 2+ indicator, fura-2. ] i is associated with the activation of the voltagedependent Ca 2+ channels in rat ORNs.

Cited by 16 publications

References 35 publications

Neurons growing from neonatal murine olfactory explant culture responded to forskolin

Neurons growing from neonatal murine olfactory explant culture responded to forskolin

Voltage-Activated Calcium Channels as Functional Markers of Mature Neurons in Human Olfactory Neuroepithelial Cells: Implications for the Study of Neurodevelopment in Neuropsychiatric Disorders

Interplay among cGMP, cAMP, and Ca2+ in Living Olfactory Sensory Neurons In Vitro and In Vivo

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