Characterization of Five Novel Brevibacillus Bacteriophages and Genomic Comparison of Brevibacillus Phages

Abstract

Brevibacillus laterosporus is a spore-forming bacterium that causes a secondary infection in beehives following European Foulbrood disease. To better understand the contributions of Brevibacillus bacteriophages to the evolution of their hosts, five novel phages (Jenst, Osiris, Powder, SecTim467, and Sundance) were isolated and characterized. When compared with the five Brevibacillus phages currently in NCBI, these phages were assigned to clusters based on whole genome and proteome synteny. Powder and Osiris, both myoviruses, were assigned to the previously described Jimmer-like cluster. SecTim467 and Jenst, both siphoviruses, formed a novel phage cluster. Sundance, a siphovirus, was assigned as a singleton phage along with the previously isolated singleton, Emery. In addition to characterizing the basic relationships between these phages, several genomic features were observed. A motif repeated throughout phages Jenst and SecTim467 was frequently upstream of genes predicted to function in DNA replication, nucleotide metabolism, and transcription, suggesting transcriptional co-regulation. In addition, paralogous gene pairs that encode a putative transcriptional regulator were identified in four Brevibacillus phages. These paralogs likely evolved to bind different DNA sequences due to variation at amino acid residues predicted to bind specific nucleotides. Finally, a putative transposable element was identified in SecTim467 and Sundance that carries genes homologous to those found in Brevibacillus chromosomes. Remnants of this transposable element were also identified in phage Jenst. These discoveries provide a greater understanding of the diversity of phages, their behavior, and their evolutionary relationships to one another and to their host. In addition, they provide a foundation with which further Brevibacillus phages can be compared.

Honey bees harbor a diverse gut virome engaging in nested strain-level interactions with the microbiota

“…The only studies regarding honey bee-associated phages are those from the pathogens Paenibacillus larvae (43)(44)(45) and Brevibacillus laterosporus (46,47). Phages associated with the specialized gut microbiota of honey bees have not been studied to date.…”

Efficacy of vaporized hydrogen peroxide for repeated sterilization of a single‐use single‐incision laparoscopic surgery port

“…Brevibacillus is a grampositive, aerobic bacillus commonly found in soil and rarely associated with infectious disease; it is therefore a probable environmental contaminant and not associated with inoculation and sterilization. 25,26 In the previous study using ethylene oxide for sterilization of the port, 19 a Bacillus species isolated from the negative control port was also attributed to environmental contamination. E coli isolated from the foam of the negative control port during the 10th cycle was not present in the enrichment broth for that port and likely represents cross-contamination of the plate during the culture process, particularly given that the negative control was not inoculated with E coli.…”

Bystander Phage Therapy: Inducing Host-Associated Bacteria to Produce Antimicrobial Toxins against the Pathogen Using Phages

“…Prior to this study, phages that specifically infect B. laterosporus were isolated from beehives and the genomes of most have been studied and published [19,20,21]. In this study, isolated phages were tested against strains of B. laterosporus to determine the most effective combination of phages to be included in a final cocktail.…”