Characterization of field‐evolved resistance to <i>Bacillus thuringiensis</i>‐derived Cry1F δ‐endotoxin in <scp><i>Spodoptera frugiperda</i></scp> populations from Argentina

Chandrasena, Desmi; Signorini, Ana M; Abratti, Gustavo; Storer, Nicholas P.; Olaciregui, Magdalena L; Alves, Analiza P.; Pilcher, Clinton D.

doi:10.1002/ps.4776

Cited by 129 publications

(104 citation statements)

References 31 publications

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“…The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae: Noctuinae), is one of the most infamous insect pests due to an extremely high-level of polyphagy (more than 353 host-plants belonging to 76 plant families are reported 11 ), high dispersal capacity and migratory behavior 12 , the rapid development of insecticide resistance 13,14 , including resistance to Bt proteins [15][16][17][18] , and occasional outbreaks 19 . The FAW is native to North and South America, and its presence in West Africa was first reported in 2016 20 .…”

Section: Textmentioning

confidence: 99%

Genomic balancing selection is key to the invasive success of the fall armyworm

Yainna

Tay

Fiteni

et al. 2020

Preprint

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A successful biological invasion involves survival in a newly occupied environment. If a population bottleneck occurs during an invasion, the resulting depletion of genetic variants could cause increased inbreeding depression and decreased adaptive potential, which may result in a fitness reduction. How invasive populations survive in the newly occupied environment despite reduced heterozygosity and how, in many cases, they maintain moderate levels of heterozygosity are still contentious issues 1 . The Fall armyworm (FAW; Lepidoptera: Spodoptera frugiperda), a polyphagous pest, is native to the Western hemisphere. Its invasion in the Old World was first reported from West Africa in early 2016, and in less than four years, it swept sub-Saharan Africa and Asia, finally reaching Australia. We used population genomics approaches to investigate the factors that may explain the invasive success of the FAW. Here we show that genomic balancing selection played a key role in invasive success by restoring heterozygosity before the global invasion. We observe a drastic loss of mitochondrial polymorphism in invasive populations, whereas nuclear heterozygosity exhibits a mild reduction. The population from Benin in West Africa has the lowest length of linkage disequilibrium amongst all invasive and native populations despite its reduced population size. This result indicates that balancing selection increased heterozygosity by facilitating the admixture of invasive populations from distinct origins and that, once heterozygosity was sufficiently high, FAW started spreading globally in the Old World. As comparable heterozygosity levels between invasive and native populations are commonly observed 1 , we postulate that the restoration of heterozygosity through balancing selection could be widespread among successful cases of biological invasions.

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Section: Textmentioning

confidence: 99%

Genomic balancing selection is key to the invasive success of the fall armyworm

Yainna

Tay

Fiteni

et al. 2020

Preprint

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“…Laboratory studies have also indicated resistance of FAW to YieldGard VT PRO maize that expresses Cry1A.105 and Cry2Ab2, PowerCore containing Cry1A.105, Cry2Ab2 and Cry1F, and Agrisure Viptera and Agrisure Viptera 3 that express Vip3Aa20 and Vip3Aa20/Cry1Ab, respectively . FAW also evolved resistance to Cry1F maize in Puerto Rico, in some areas of the southeastern USA, and Argentina …”

Section: Introductionmentioning

confidence: 99%

Laboratory and field survival of Spodoptera frugiperda (Lepidoptera: Noctuidae) on Bt and non‐Bt maize and its susceptibility to insecticides

Muraro

Garlet

Godoy

et al. 2019

Pest Management Science

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BACKGROUND: Field-evolved resistance of fall armyworm (FAW), Spodoptera frugiperda (Smith), has been reported to Bt maize technologies in Brazil. The control failures of FAW by Bt maize increased the use of insecticides for their control. However, no information is available on the interaction between resistant FAW and their response to insecticides. Here, we evaluated the survival of FAW strains on Bt and non-Bt maize in laboratory and field conditions, and their susceptibility to insecticides. RESULTS: In the laboratory, resistant FAW larvae reared on Bt and non-Bt maize showed a similar susceptibility to spinetoram (LC 50 = 0.16-0.18 g a.i. cm −2 ) and chlorfenapyr (LC 50 = 0.17-0.20 g a.i. cm −2 ). However, their susceptibility was lower than that of the susceptible strain reared on non-Bt maize: LC 50 = 0.05 (spinetoram) and 0.08 (chlorfenapyr) g a.i. cm −2 . In contrast, heterozygous strains had similar susceptibility to the susceptible strain. In field trials, no differences in FAW survival were detected between strains when the commercial dose of two insecticides was applied in Bt and non-Bt maize.CONCLUSION: FAW strains surviving on Bt and non-Bt maize, at the same development stage, have similar susceptibility to insecticides. The integrated pest management practices and insect resistance management importance of these results are discussed.

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“…At present, a number of field-evolved resistant populations of fall armyworm have been detected, including resistance to a variety of chemical pesticides and Bt crops, and the level of resistance is increasing [25][26][27][28][29] . The mechanisms of resistance to pesticides are mainly due to variation in receptor genes, such as amino acid mutations in the ryanodine receptor (RyR) (diamide), acetylcholinesterase (AChE) (organophosphate), voltagegated sodium channel (VGSC) (pyrethroids), and so on [30][31][32] .…”

Section: Introductionmentioning

confidence: 99%

High-depth resequencing reveals hybrid population and insecticide resistance characteristics of fall armyworm (Spodoptera frugiperda) invading China

Zhang

Liu

Zheng

et al. 2019

Preprint

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The rapid wide-scale spread of fall armyworm (Spodoptera frugiperda) has caused serious crop losses globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report a 393.25-M chromosome-level genome assembly of fall armyworm with scaffold N50 of 13.3 M consisting of 23281 annotated protein-coding genes. Genome-wide resequencing of 105 samples from 16 provinces in China revealed that the fall armyworm population comprises a complex inter-strain hybrid, mainly with the corn-strain genetic background and less of the rice-strain genetic background, which highlights the inaccuracy of strain identification using mitochondrial or Tpi genes. An analysis of genes related to pesticide-and Bt-resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high, while remaining currently susceptible to Bt toxins. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, which is consistent with the results of molecular scanning of resistance-related genes, but are sensitive to genetically modified maize expressing Cry1Ab in field experiments. Additionally, we found that two mitochondrial fragments are inserted into the nuclear genome, and the insertion event occurred after the differentiation of the two strains. This study represents a valuable advancement toward the analysis of genetic differences among subpopulations and improving management strategies for fall armyworm.

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Characterization of field‐evolved resistance to Bacillus thuringiensis‐derived Cry1F δ‐endotoxin in Spodoptera frugiperda populations from Argentina

Cited by 129 publications

References 31 publications

Genomic balancing selection is key to the invasive success of the fall armyworm

Genomic balancing selection is key to the invasive success of the fall armyworm

Laboratory and field survival of Spodoptera frugiperda (Lepidoptera: Noctuidae) on Bt and non‐Bt maize and its susceptibility to insecticides

High-depth resequencing reveals hybrid population and insecticide resistance characteristics of fall armyworm (Spodoptera frugiperda) invading China

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