Characterization of fertility alteration and marker validation for male sterility genes in novel PTGMS lines hybrid rice

Abstract

Photoperiod and thermosensitive genetic male sterile (PTGMS) lines have become one of the main sources of global rice production increasing. This study was conducted to evaluate the fertility alteration and validate the male sterility genes using validation markers in novel Egyptian Indica and Japonica PTGMS lines under natural conditions. The study revealed that the new genetic male sterile lines belong to the type of photo–thermosensitive genetic male sterility (PTGMS). The fertility alteration of these lines has influenced by photoperiod and temperature interaction. The new PTGMS lines have three sensitive periods of fertility alteration; transformation, sterility, and fertility period. Furthermore, the sensitive stage of fertility transformation might be from secondary branch primordial to pollen mother cells (PMC) meiosis. Under the natural Sakha condition, the new PTGMS lines were stable sterile under the condition of day length upper 13,75 h and temperature over 25 °C, while its convert to fertile under day length under 13 h, and temperature lower than 24 °C. The co-dominant markers identified the pms3 and tms5 genes in the new PTGMS lines, indicated that the fertility alteration in these lines controlled by photoperiod and thermosensitive stages.

Development of Two Novel Photoperiod and Thermo-Sensitive Genic Male Sterile Lines in Rice (Oryza sativa L.)

“…While, the panicle length, grain length, grain width, and grain thickness traits were not significant heterosis. Anis et al, 2017, El-Mowafi et al, 2021and Gaballah et al, 2022a and manyother rice breeders agreed with the importance of heterosis to increase the yield potential.This increasearound 20% forthree-line systems and approximately 40 % for twoline systems due to the hybrid vigor in the progeny of diverse varieties of species or crosses between species exhibit greater biomass, yield and fertility than both parents.…”

“…We can conclude from the presented results that the new PTGMS lines are promising for hybrid rice production as a female parent. Under Egyptian conditions, many PTGMS were developed by (El-Mowafi et al, 2012;El-Mowafi et al, 2021).…”

“…The two PTGMS fertility alternated from completely sterile to partially fertile in late July when the temperature decreased, and the day length in late August became shorter (Figures 5 and 6). For Fertilitysterility alternation, characterization uses two main methods, the first under normal field conditions depending on temperature variation and daylength changing throughout the growing season as previously applied by El-Mowafi et al, 2021;Li et al, 2020. While the second conducting under controlled conditions in growth chambers, as reported by Liu et al, 2021;Yang et al, 2021. The field Performance and grain characterization of Derived Hybrid combinations Two hybrid rice combinations were derived from the new PTGMS linesA001S and G001S.…”