Characterization of extracts from winery by-products with antifungal activity against Botrytis cinerea

Mendoza, Leonora; Yáñez, Karen P.; Vivanco, Marcela; Melo, Ricardo; Cotoras, Milena

doi:10.1016/j.indcrop.2012.07.048

Cited by 63 publications

(75 citation statements)

References 23 publications

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“…It is worth noting that the extracts produced with water presented low antioxidant potential and also low content of total phenols when compared with most of the extracts produced with organic solvents like ethanol. Similar results have been reported during the extraction of antioxidant compounds from other raw materials such as spent coffee grounds (Mussatto et al, 2011;Panusa et al, 2013), grape by-products (Mendoza et al, 2013), or almond (Sfahlan et al, 2009).…”

Section: 36supporting

confidence: 86%

Coffee silverskin: A possible valuable cosmetic ingredient

Rodrigues

Palmeira‐de‐Oliveira

Neves

et al. 2014

Pharmaceutical Biology

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Context: Currently, there is a great tendency in cosmetic area to use natural extracts. Coffee silverskin (CS) is the most abundant solid by-product generated during roasting of coffee processing. Objectives: To evaluate different CS extracts as promising cosmetic ingredients, regarding antioxidant, antimicrobial, and cytotoxic properties. Materials and methods: Aqueous, hydroalcoholic and ethanolic CS extracts were obtained by an environmentally friendly procedure considering costs and pollution. Extracts were characterized for total phenolic and flavonoid contents (TPC and TFC, respectively), antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), antimicrobial activity expressed as minimal inhibitory concentration (MIC) and cytotoxicity using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) and lactate dehydrogenase (LDH) assays in two skin cell lines (fibroblasts and keratinocytes). Results: The TPC of extracts was 18.33-35.25 mg of gallic acid equivalents per g of material on a dry basis (mg GAE/g db). The TFC of extracts was 1.08-2.47 mg cathechin equivalents per g dry material (mg CE/g db). The antioxidant activity was high, with values ranging between 95.95 and 216.40 mmol Fe 2+ /g for aqueous and alcoholic samples, respectively. Preliminary assays for antimicrobial potential showed that extracts display antibacterial activity. The MIC varied from 31.3 to 250 mg/mL for Gram-positive, and from 31.3 to 1000 mg/mL for Gram-negative. Extracts did not affect in vitro cell viability, with values near 100% in all concentrations tested. Conclusion: Results seem show that CS is a safe source of natural antioxidants with antifungal and antibacterial activity and no cytotoxicity, with potential usefulness for cosmetic applications.

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Section: 36supporting

confidence: 86%

Coffee silverskin: A possible valuable cosmetic ingredient

Rodrigues

Palmeira‐de‐Oliveira

Neves

et al. 2014

Pharmaceutical Biology

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“…It has been reported that extracts obtained from grape pomaces present antifungal activity against B. cinerea. However, pure compounds identified in these extracts showed low antifungal effect 7 . Numerous authors have reported that the biological activity of phenolic compounds can be improved modifying phenolic molecules by using the enzyme laccase (EC 1.10.3.2) a polyphenol oxidase 8 .…”

Section: Introductionmentioning

confidence: 99%

Improvement of the Antifungal Activity Against Botrytis Cinerea of Syringic Acid, a Phenolic Acid From Grape Pomace

Mendoza

Castro

Melo

et al. 2016

J. Chil. Chem. Soc.

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The aim of this study was to improve the antifungal activity against Botrytis cinerea of syringic acid by using the enzyme laccase to synthesize a heterodimeric compound by a coupling reaction with aniline. The synthesized heterodimer is a quinone-imine like compound (2,6-dimethoxy-4-(phenylimino)benzenone), which was characterized by using 1 H and 13 C NMR, IR, and mass spectrometry. The antifungal activity of the heterodimeric compound against B. cinerea was determined in vitro and showed a higher antifungal effect than the substrates (syringic acid and aniline), inhibiting the mycelial growth with an IC 50 value of 0.14mM and delayed 2 hours the conidial germination. Also, using the fluorochrome calcofluor white, cell wall damage was observed when B. cinerea was incubated with the compound. This is the first report on antifungal activity of this type of compound against B. cinerea.

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“…El efecto inhibitorio del desarrollo de B. cinerea de los EHVS empleados puede deberse a la presencia de ácido gálico, ferúlico y resveratrol, polifenoles que han mostrado propiedades antifúngicas (Katalinić et al, 2009;Schnee et al, 2013;Mendoza et al, 2013). Vio-Michaelis et al (2012), indicaron la presencia de ácido ferúlico en los extractos etanólicos de dos especies vegetales que poseen potencial para el control de B. cinerea; mientras identified, is mainly the result of genetics, as the plant tissues of the three accessions came from the same germplasm bank.…”

Section: Discussionunclassified

“…In particular, Vitis vinifera has been reported to produce several compounds with antimicrobial and antifungal activity, such as myricetin, ellagic acid, kaempferol, quercitin, and gallic acid, among others (Schnee et al, 2013). Within the phenolic compounds present in fruit peel extracts of a mix of three Chilean cultivars of V. vinifera, p-coumaric acid (98.5 µg/ mL) and kaempferol (100.9 µg/mL) showed the highest antifungal activity; the phenolic extract was reported to have potential against B. cinerea (Mendoza et al, 2013).…”

Section: In Vitro Inhibition Ofmentioning

confidence: 99%

“…Revista Mexicana de fitoPatología Mexican JouRnal of PhytoPathology fenólicos presentes en extractos de cáscara de una mezcla de tres cultivares chilenos de V. vinifera se reportó la mayor actividad antifúngica de ácido pcoumarico (98.5 µg/mL) y kaempferol (100.9 µg/ mL); el extracto fenólico fue reportado con potencial contra B. cinerea (Mendoza et al, 2013).…”

Section: Fully Bilingualunclassified

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Inhibición in vitro de Botrytis cinerea con extractos de hojas de vid silvestre (Vitis spp.)

Rodríguez¹,

Mora²,

Siclán³

et al. 2017

RMF

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Abstract. The extracts of wild grapevines (Vitis) leaves (EHVS) from three accessions (P-178, E-200 and TN-4) at 6, 8 and 12 % v/v, were tested in vitro to evaluate their inhibitory effect on Botrytis cinerea development. Resveratrol (RVS) (60, 90, 120 µg/mL), citrus extracts (EC) (3, 5 and 8 % v/v) and cyprodinil + fludioxonil (SW) (500, 800 and 1000 µg/mL) were compared against EHVS. In average, SW presented inhibition of mycelial growth (ICM), sporulation (IE) and spore germination (IGC) (88.9; 85.5 and 93.7 %, average respectively). RVS presented the second higher inhibition rate. All EHVS presented antifungal activity; specially, P-178 at 12 % resulted in 72 % ICM, 75 % IE and 62 % IGC. This dose contained phenolic compounds 19.9 mg/mL, RVS 1.7 mg/mL, gallic acid 3.8 mg/mL and ferulic acid 2.5 mg/mL.

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Characterization of extracts from winery by-products with antifungal activity against Botrytis cinerea

Cited by 63 publications

References 23 publications

Coffee silverskin: A possible valuable cosmetic ingredient

Coffee silverskin: A possible valuable cosmetic ingredient

Improvement of the Antifungal Activity Against Botrytis Cinerea of Syringic Acid, a Phenolic Acid From Grape Pomace

Inhibición in vitro de Botrytis cinerea con extractos de hojas de vid silvestre (Vitis spp.)

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