Characterization of Extracellular Proteases ofAeromonas hydrophila

Proteases of psychrotrophic bacteria isolated from glaciers

“…1972, ALICHANIDIS and ANDREWS 1977, PATEL et al 1983a, 1983b and fish (GENICOT et al 1988) exhibited highest activity at temperatures around 40-45 "C. We obtained similar results: 10 crude proteases of. psychrotrophs showed a maximum activity at 40 "C, 5 at 45 "C, 4 at 35 "C, and 3 a t -30 "C. The temperature optimum of one protease was at 50 "C, and in 2 cases at 60 "C. For proteases from mesophilic Gram negative bacteria of the genera Pseudomonas (MORIHARA 1963, BOETHLING 1975 and Aeromonas (PANSARE et al 1986) temperature optima of 50-60 "C were reported. Subtilisin C. and Savinase which are produced by mesophilic strains of Bacillus sp., showed a temperature optimum of 60 "C, confirming the results obtained by AUNSTRUP (1980).…”

“…Besides the pH-neutral conditions when isolating and cultivating, also the conditions of the natural environments of the strains were responsible for this result. The temperature optimum of one protease was at 50 "C, and in 2 cases at 60 "C. For proteases from mesophilic Gram negative bacteria of the genera Pseudomonas (MORIHARA 1963, BOETHLING 1975 and Aeromonas (PANSARE et al 1986) temperature optima of 50-60 "C were reported. Subtilisin C. and Savinase which are produced by mesophilic strains of Bacillus sp., showed a temperature optimum of 60 "C, confirming the results obtained by AUNSTRUP (1980).…”

Aeromonas hydrophìla, A human pathogen

“…20 Οι εξωτοξίνες που παράγονται από A hydrophila εί ναι: α) αιμολυσίνη, β) κυτοτοξίνη, γ) εντεροτοξίνη και δ) κυτοτονική τοξίνη (Cholera toxin-like factor) 29 , ε) πρωτε-άση . 30 Ο Agger και συν. (1985) διαπίστωσαν ότι ποσοστό 62% των στελεχών χτ\ςΑ.…”

“…2837 Η θνη τότητα λόγω παρεντερικών λοιμώξεων σε ανοσοκατα σταλμένα άτομα είναι δυνατόν να υπερβαίνει το 60%. 30 Πύ λη εισόδου όσον άφορα τις μεταδοτικές (διάσπαρτες) λοι μώξεις είναι δυνατόν να είναι η γαστρεντερική οδός, λόγω κατανάλωσης τροφίμων μολυσμένων με αερομονάδες.…”